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Vernalophrys algivore gen. nov., sp nov (Rhizaria: Cercozoa: Vampyrellida), a New Algal Predator Isolated from Outdoor Mass Culture of Scenedesmus dimorphus
Gong, Yingchun1,2,3; Patterson, David J.4; Li, Yunguang5; Hu, Zixuan6; Sommerfeld, Milton3; Chen, Yongsheng6; Hu, Qiang1,2,3; Gong, YC (reprint author), Chinese Acad Sci, Inst Hydrobiol, Ctr Microalgal Biotechnol & Biofuels, Wuhan, Peoples R China.
2015-06-01
Source PublicationAPPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN0099-2240
Volume81Issue:12Pages:3900-3913
AbstractMicrobial contamination is the main cause of loss of biomass yield in microalgal cultures, especially under outdoor environmental conditions. Little is known about the identities of microbial contaminants in outdoor mass algal cultures. In this study, a new genus and species of vampyrellid amoeba, Vernalophrys algivore, is described from cultures of Scenedesmus dimorphus in open raceway ponds and outdoor flat-panel photobioreactors. This vampyrellid amoeba was a significant grazer of Scenedesmus and was frequently associated with a very rapid decline in algal numbers. We report on the morphology, subcellular structure, feeding behavior, molecular phylogeny, and life cycle. The new amoeba resembles Leptophrys in the shape of trophozoites and pseudopodia and in the mechanism of feeding (mainly by engulfment). It possesses two distinctive regions in helix E10_1 (nucleotides 117 to 119, CAA) and E23_1 (nucleotides 522 and 523, AG) of the 18S rRNA gene. It did not form a monophyletic group with Leptophrys in molecular phylogenetic trees. We establish a new genus, Vernalophrys, with the type species Vernalophrys algivore. The occurrence, impact of the amoeba on mass culture of S. dimorphus, and means to reduce vampyrellid amoeba contamination in Scenedesmus cultures are addressed. The information obtained from this study will be useful for developing an early warning system and control measures for preventing or treating this contaminant in microalgal mass cultures.
SubtypeArticle
Department[Gong, Yingchun ; Hu, Qiang] Chinese Acad Sci, Inst Hydrobiol, Ctr Microalgal Biotechnol & Biofuels, Wuhan, Peoples R China ; [Gong, Yingchun ; Hu, Qiang] Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Peoples R China ; [Gong, Yingchun ; Sommerfeld, Milton ; Hu, Qiang] Arizona State Univ, Coll Technol & Innovat, Lab Algae Res & Biotechnol, Mesa, AZ USA ; [Patterson, David J.] Univ Sydney, Sch Biol Sci, Sydney, NSW 2006, Australia ; [Li, Yunguang] Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan, Peoples R China ; [Hu, Zixuan ; Chen, Yongsheng] Georgia Inst Technol, Sch Civil & Environm Engn, Atlanta, GA 30332 USA
DOI10.1128/AEM.00160-15
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Microbiology
WOS SubjectBiotechnology & Applied Microbiology ; Microbiology
WOS IDWOS:000354864000001
WOS KeywordPHYLOGENETIC TREES ; SEQUENCE ALIGNMENT ; FILOSE AMEBAS ; NAKED FILOSE ; PARASITES ; GROWTH ; CILIOPHORA ; INTERFACE ; INFERENCE ; POSITION
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23878
Collection藻类生物技术和生物能源研发中心_期刊论文
Corresponding AuthorGong, YC (reprint author), Chinese Acad Sci, Inst Hydrobiol, Ctr Microalgal Biotechnol & Biofuels, Wuhan, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Ctr Microalgal Biotechnol & Biofuels, Wuhan, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Peoples R China
3.Arizona State Univ, Coll Technol & Innovat, Lab Algae Res & Biotechnol, Mesa, AZ USA
4.Univ Sydney, Sch Biol Sci, Sydney, NSW 2006, Australia
5.Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan, Peoples R China
6.Georgia Inst Technol, Sch Civil & Environm Engn, Atlanta, GA 30332 USA
Recommended Citation
GB/T 7714
Gong, Yingchun,Patterson, David J.,Li, Yunguang,et al. Vernalophrys algivore gen. nov., sp nov (Rhizaria: Cercozoa: Vampyrellida), a New Algal Predator Isolated from Outdoor Mass Culture of Scenedesmus dimorphus[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2015,81(12):3900-3913.
APA Gong, Yingchun.,Patterson, David J..,Li, Yunguang.,Hu, Zixuan.,Sommerfeld, Milton.,...&Gong, YC .(2015).Vernalophrys algivore gen. nov., sp nov (Rhizaria: Cercozoa: Vampyrellida), a New Algal Predator Isolated from Outdoor Mass Culture of Scenedesmus dimorphus.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,81(12),3900-3913.
MLA Gong, Yingchun,et al."Vernalophrys algivore gen. nov., sp nov (Rhizaria: Cercozoa: Vampyrellida), a New Algal Predator Isolated from Outdoor Mass Culture of Scenedesmus dimorphus".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 81.12(2015):3900-3913.
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