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题名: A proteomic analysis of prenatal transfer of microcystin-LR induced neurotoxicity in rat offspring
作者: Zhao, Sujuan1, 2; Li, Guangyu3; Chen, Jun1
通讯作者: Li, GY (reprint author), Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China.
关键词: Neurotoxicity ; MCLR ; Proteomics ; Offspring ; Generation transfer
刊名: JOURNAL OF PROTEOMICS
发表日期: 2015-01-30
DOI: 10.1016/j.jprot.2014.11.015
卷: 114, 页:197-213
收录类别: SCI
文章类型: Article
部门归属: [Zhao, Sujuan ; Chen, Jun] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China ; [Zhao, Sujuan] Anhui Med Univ, Sch Publ Hlth, Hefei 230032, Peoples R China ; [Li, Guangyu] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Recent studies showed that microcystins (MCs) can be transferred to offspring from their adults and exert notable neurotoxicity, but the exact mechanism is little known. In order to better understand cellular responses in brain tissues disrupted by prenatal transfer of MCs, this work mainly focuses on brain impairments of rat offspring. Pregnant SD rats were infused exposed to microcystin-LR (MCLR) at 10 mu g/kg body weight (BW)/day or saline solution from gestational day 8 (GD8) to postnatal day 15 (PD15) of lactation. MCLR accumulation, the levels of malondialdehyde (MDA) and acetylcholine esterase (AChE) activity were detected. The results showed that MCLR enhanced toxin accumulation and MDA, but decreased GSH and the level of AChE activity in the brains of rat offspring. MCLR also caused changes to cerebrum ultrastructure, showing a sparse structure, distention of endoplasmic reticulum and swelling mitochondria. To explore the exact mechanisms, we used a proteomic analysis to identify global brain protein profiles. The proteomic results revealed that MCLR remarkably altered the abundance of 49 proteins that were involved in neurodevelopment, oxidative phosphorylation, cytoskeleton, metabolism, protein folding and degradation. Our results indicated that MCLR exerts neurotoxicity mainly by generating oxidative stress and endoplasmic reliculum stress.
关键词[WOS]: OXIDATIVE STRESS GENERATION ; INDUCED APOPTOSIS ; ALZHEIMERS-DISEASE ; DANIO-RERIO ; PROTEIN EXPRESSION ; ORYZIAS-LATIPES ; MITOCHONDRIAL ; TOXICITY ; BRAIN ; RR
语种: 英语
WOS记录号: WOS:000348949100016
ISSN号: 1874-3919
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/23872
Appears in Collections:淡水生态学研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
2.Anhui Med Univ, Sch Publ Hlth, Hefei 230032, Peoples R China
3.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
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