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A proteomic analysis of prenatal transfer of microcystin-LR induced neurotoxicity in rat offspring
Zhao, Sujuan1,2; Li, Guangyu3; Chen, Jun1; Li, GY (reprint author), Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China.
2015-01-30
Source PublicationJOURNAL OF PROTEOMICS
ISSN1874-3919
Volume114Pages:197-213
AbstractRecent studies showed that microcystins (MCs) can be transferred to offspring from their adults and exert notable neurotoxicity, but the exact mechanism is little known. In order to better understand cellular responses in brain tissues disrupted by prenatal transfer of MCs, this work mainly focuses on brain impairments of rat offspring. Pregnant SD rats were infused exposed to microcystin-LR (MCLR) at 10 mu g/kg body weight (BW)/day or saline solution from gestational day 8 (GD8) to postnatal day 15 (PD15) of lactation. MCLR accumulation, the levels of malondialdehyde (MDA) and acetylcholine esterase (AChE) activity were detected. The results showed that MCLR enhanced toxin accumulation and MDA, but decreased GSH and the level of AChE activity in the brains of rat offspring. MCLR also caused changes to cerebrum ultrastructure, showing a sparse structure, distention of endoplasmic reticulum and swelling mitochondria. To explore the exact mechanisms, we used a proteomic analysis to identify global brain protein profiles. The proteomic results revealed that MCLR remarkably altered the abundance of 49 proteins that were involved in neurodevelopment, oxidative phosphorylation, cytoskeleton, metabolism, protein folding and degradation. Our results indicated that MCLR exerts neurotoxicity mainly by generating oxidative stress and endoplasmic reliculum stress.
SubtypeArticle
KeywordNeurotoxicity Mclr Proteomics Offspring Generation Transfer
Department[Zhao, Sujuan ; Chen, Jun] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China ; [Zhao, Sujuan] Anhui Med Univ, Sch Publ Hlth, Hefei 230032, Peoples R China ; [Li, Guangyu] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
DOI10.1016/j.jprot.2014.11.015
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemical Research Methods
WOS IDWOS:000348949100016
WOS KeywordOXIDATIVE STRESS GENERATION ; INDUCED APOPTOSIS ; ALZHEIMERS-DISEASE ; DANIO-RERIO ; PROTEIN EXPRESSION ; ORYZIAS-LATIPES ; MITOCHONDRIAL ; TOXICITY ; BRAIN ; RR
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23872
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorLi, GY (reprint author), Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
2.Anhui Med Univ, Sch Publ Hlth, Hefei 230032, Peoples R China
3.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Sujuan,Li, Guangyu,Chen, Jun,et al. A proteomic analysis of prenatal transfer of microcystin-LR induced neurotoxicity in rat offspring[J]. JOURNAL OF PROTEOMICS,2015,114:197-213.
APA Zhao, Sujuan,Li, Guangyu,Chen, Jun,&Li, GY .(2015).A proteomic analysis of prenatal transfer of microcystin-LR induced neurotoxicity in rat offspring.JOURNAL OF PROTEOMICS,114,197-213.
MLA Zhao, Sujuan,et al."A proteomic analysis of prenatal transfer of microcystin-LR induced neurotoxicity in rat offspring".JOURNAL OF PROTEOMICS 114(2015):197-213.
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