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Sources of organic matter affect depth-related microbial community composition in sediments of Lake Erhai, Southwest China
Xiong, Wei1; Xie, Ping2; Wang, Shengrui3; Niu, Yuan3; Yang, Xi2; Chen, Wenjie1; Xie, P (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China.
2015
Source PublicationJOURNAL OF LIMNOLOGY
ISSN1129-5767
Volume74Issue:2Pages:310-323
AbstractSediment cores taken from different areas of the mesotrophic Lake Erhai were analysed to investigate the vertical distribution of bacterial community composition (BCC), as well as physicochemical parameters. PCR-denaturing gradient gel electrophoresis (DGGE), stable carbon isotope (delta C-13), C/N atomic ratio and canonical correspondence analysis (CCA) were used to explore the relationships between the succession of bacterial communities and environmental variables, emphasising changes in the sources of organic matter (OM). The BCC in natural environments was characterised by DGGE of the 16S rRNA gene with subsequent sequencing of bands of interest. The CCA revealed that the depth-related variation in sediment bacterial communities in different areas of the lake was significantly influenced by varying environmental factors. The OM source, however, played an important role in structuring BCC at all sites. The DGGE banding patterns revealed that the abundance of Deltaproteobacteria decreased with accompanying elevated levels of C4 plant-derived organic carbon. The sequencing of DGGE bands suggested that the majority of the sequences were affiliated with common phylogenetic groups in lake sediments: Chloroflexi, Deltaproteobacteria and Firmicutes. Betaproteobacteria detected in our study appeared as a prominent phylotype in the upper sediment. The Shannon-Wiener diversity index of bacterial communities was directly affected by the OM source. Constant OM sources resulted in a stable higher diversity of bacterial communities and broader enzymatic capabilities to access OM. We conclude that the differences in the diversity of bacterial communities in sediments differing in their sources of OM were related to environmental variables (e.g. water level, river runoff and terrestrial vegetation composition). Our study provided insights into the relationships between natural BCC and OM sources, facilitating a better understanding of microbial community structure in lake sediment.
SubtypeArticle
KeywordBacterial Community Composition Organic Matter Denaturing Gradient Gel Electrophoresis Delta C-13 Canonical Correspondence Analysis Sediment
Department[Xiong, Wei ; Chen, Wenjie] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China ; [Xie, Ping ; Yang, Xi] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China ; [Wang, Shengrui ; Niu, Yuan] Chinese Res Inst Environm Sci, Res Ctr Lake Ecoenvironm, State Environm Protect Key Lab Lake Pollut Contro, Beijing 100012, Peoples R China
DOI10.4081/jlimnol.2014.1106
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaMarine & Freshwater Biology
WOS SubjectLimnology
WOS IDWOS:000355151500011
WOS KeywordGRADIENT GEL-ELECTROPHORESIS ; RIBOSOMAL-RNA FRAGMENTS ; CARBON ISOTOPES ; LATE QUATERNARY ; 16S RDNA ; BACTERIAL ; DIVERSITY ; BACTERIOPLANKTON ; DYNAMICS ; SHALLOW
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23852
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorXie, P (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China.
Affiliation1.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
3.Chinese Res Inst Environm Sci, Res Ctr Lake Ecoenvironm, State Environm Protect Key Lab Lake Pollut Contro, Beijing 100012, Peoples R China
Recommended Citation
GB/T 7714
Xiong, Wei,Xie, Ping,Wang, Shengrui,et al. Sources of organic matter affect depth-related microbial community composition in sediments of Lake Erhai, Southwest China[J]. JOURNAL OF LIMNOLOGY,2015,74(2):310-323.
APA Xiong, Wei.,Xie, Ping.,Wang, Shengrui.,Niu, Yuan.,Yang, Xi.,...&Xie, P .(2015).Sources of organic matter affect depth-related microbial community composition in sediments of Lake Erhai, Southwest China.JOURNAL OF LIMNOLOGY,74(2),310-323.
MLA Xiong, Wei,et al."Sources of organic matter affect depth-related microbial community composition in sediments of Lake Erhai, Southwest China".JOURNAL OF LIMNOLOGY 74.2(2015):310-323.
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