IHB OpenIR  > 淡水生态学研究中心  > 期刊论文
Taxonomic Resolutions Based on 18S rRNA Genes: A Case Study of Subclass Copepoda
Wu, Shu1,2; Xiong, Jie1; Yu, Yuhe1; Yu, YH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Peoples R China.
2015-06-24
Source PublicationPLOS ONE
ISSN1932-6203
Volume10Issue:6Pages:e0131498
AbstractBiodiversity studies are commonly conducted using 18S rRNA genes. In this study, we compared the inter-species divergence of variable regions (V1-9) within the copepod 18S rRNA gene, and tested their taxonomic resolutions at different taxonomic levels. Our results indicate that the 18S rRNA gene is a good molecular marker for the study of copepod biodiversity, and our conclusions are as follows: 1) 18S rRNA genes are highly conserved intraspecies (intra-species similarities are close to 100%); and could aid in species-level analyses, but with some limitations; 2) nearly-whole-length sequences and some partial regions (around V2, V4, and V9) of the 18S rRNA gene can be used to discriminate between samples at both the family and order levels (with a success rate of about 80%); 3) compared with other regions, V9 has a higher resolution at the genus level (with an identification success rate of about 80%); and 4) V7 is most divergent in length, and would be a good candidate marker for the phylogenetic study of Acartia species. This study also evaluated the correlation between similarity thresholds and the accuracy of using nuclear 18S rRNA genes for the classification of organisms in the subclass Copepoda. We suggest that sample identification accuracy should be considered when a molecular sequence divergence threshold is used for taxonomic identification, and that the lowest similarity threshold should be determined based on a pre-designated level of acceptable accuracy.
SubtypeArticle
Department[Wu, Shu ; Xiong, Jie ; Yu, Yuhe] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Peoples R China ; [Wu, Shu] Univ Chinese Acad Sci, Beijing, Peoples R China
DOI10.1371/journal.pone.0131498
WOS HeadingsScience & Technology
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000356932500164
WOS KeywordINTERNAL TRANSCRIBED SPACER ; MITOCHONDRIAL-DNA SEQUENCES ; SPECIES DELIMITATION ; PHYLOGENETIC-RELATIONSHIPS ; CRUSTACEA COPEPODA ; MOLECULAR-DATA ; BIODIVERSITY ASSESSMENT ; EUKARYOTIC DIVERSITY ; BAYESIAN-INFERENCE ; RDNA
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23847
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorYu, YH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Wu, Shu,Xiong, Jie,Yu, Yuhe,et al. Taxonomic Resolutions Based on 18S rRNA Genes: A Case Study of Subclass Copepoda[J]. PLOS ONE,2015,10(6):e0131498.
APA Wu, Shu,Xiong, Jie,Yu, Yuhe,&Yu, YH .(2015).Taxonomic Resolutions Based on 18S rRNA Genes: A Case Study of Subclass Copepoda.PLOS ONE,10(6),e0131498.
MLA Wu, Shu,et al."Taxonomic Resolutions Based on 18S rRNA Genes: A Case Study of Subclass Copepoda".PLOS ONE 10.6(2015):e0131498.
Files in This Item:
File Name/Size DocType Version Access License
Taxonomic Resolution(1027KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wu, Shu]'s Articles
[Xiong, Jie]'s Articles
[Yu, Yuhe]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wu, Shu]'s Articles
[Xiong, Jie]'s Articles
[Yu, Yuhe]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wu, Shu]'s Articles
[Xiong, Jie]'s Articles
[Yu, Yuhe]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Taxonomic Resolutions Based on 18S rRNA Genes_ A Case Study of Subclass Copepoda.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.