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题名: De Novo Assembly and Characterization of Narrow-Ridged Finless Porpoise Renal Transcriptome and Identification of Candidate Genes Involved in Osmoregulation
作者: Ruan, Rui1, 2; Guo, Ai-Huan1, 2; Hao, Yu-Jiang1; Zheng, Jin-Song1; Wang, Ding1
通讯作者: Zheng, JS (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China.
关键词: renal transcriptome ; RNA-seq ; the narrow-ridged finless porpoise ; osmoregulation ; AQP2
刊名: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
发表日期: 2015
DOI: 10.3390/ijms16012220
卷: 16, 期:1, 页:2220-2238
收录类别: SCI
文章类型: Article
部门归属: [Ruan, Rui ; Guo, Ai-Huan ; Hao, Yu-Jiang ; Zheng, Jin-Song ; Wang, Ding] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China ; [Ruan, Rui ; Guo, Ai-Huan] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]: Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
英文摘要: During the evolutionary transition from land to water, cetaceans have undergone numerous critical challenges, with osmoregulation being the major one. Two subspecies of the narrow-ridged finless porpoise (Neophocaena asiaeorientalis), the freshwater Yangtze finless porpoise (N. a. asiaeorientalis, NAA) and the marine East Asian finless porpoise (N. a. sunameri, NAS), provide excellent subjects to understand the genetic basis of osmoregulatory divergence between freshwater and marine mammals. The kidney plays an important and well-established role in osmoregulation in marine mammals and thus, herein, we utilized RNA-seq to characterize the renal transcriptome and preliminarily analyze the divergence between the NAA and the NAS. Approximately 48.98 million clean reads from NAS and 49.40 million clean reads from NAA were obtained by RNA-Seq. And 73,449 (NAS) and 68,073 (NAA) unigenes were assembled. Among these annotations, 22,231 (NAS) and 21,849 (NAA) unigenes were annotated against the NCBI nr protein database. The ion channel complex GO term and four pathways were detected as relevant to osmoregulation by GO and KEGG pathway classification of these annotated unigenes. Although the endangered status of the study species prevented analysis of biological replicates, we identified nine differentially expressed genes (DEGs) that may be vital in the osmoregulation of the narrow-ridged finless porpoise and worthwhile for future studies. Of these DEGs, the differential expression and distribution of the aquaporin-2 (AQP2) in the collecting duct were verified using immunohistochemical experiments. Together, this work is the first report of renal transcriptome sequencing in cetaceans, and it will provide a valuable resource for future molecular genetics studies on cetacean osmoregulation.
关键词[WOS]: RNA-SEQ DATA ; DIFFERENTIAL EXPRESSION ANALYSIS ; RENIN-ANGIOTENSIN SYSTEM ; ATRIAL-NATRIURETIC-PEPTIDE ; UREA TRANSPORTERS ; MAMMALIAN KIDNEY ; REFERENCE GENOME ; MARINE MAMMALS ; NEOPHOCAENA ; EVOLUTION
语种: 英语
WOS记录号: WOS:000348403100127
ISSN号: 1422-0067
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/23841
Appears in Collections:水生生物多样性与资源保护研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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