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题名: Identification of a germ cell marker gene, the dead end homologue, in Chinese sturgeon Acipenser sinensis
作者: Yang Xiaoge1, 2; Yue Huamei2, 3; Ye Huan1, 2; Li Chuangju2, 3; Wei Qiwei1, 2, 3
通讯作者: Li, CJ (reprint author), Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, 8 1st Wudayuan Rd, Wuhan 430223, Peoples R China.
关键词: Dead end ; Fluorescent in situ hybridization ; Primordial germ cell ; qRT-PCR ; Chinese sturgeon
刊名: GENE
发表日期: 2015-03-01
DOI: 10.1016/j.gene.2014.12.059
卷: 558, 期:1, 页:118-125
收录类别: SCI
文章类型: Article
部门归属: [Yang Xiaoge ; Ye Huan ; Wei Qiwei] Chinese Acad Sci, Univ Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China ; [Yang Xiaoge ; Yue Huamei ; Ye Huan ; Li Chuangju ; Wei Qiwei] Chinese Acad Fishery Sci, Key Lab Freshwater Biodivers Conservat, Minist Agr China, Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China ; [Yue Huamei ; Li Chuangju ; Wei Qiwei] Chinese Acad Fisheries Sci, Freshwater Fisheries Res Ctr, Wuxi 214081, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Genetics & Heredity
研究领域[WOS]: Genetics & Heredity
英文摘要: Dead end (dnd) encodes an RNA-binding protein that is essential for primordial germ cell (PGC) migration and gametogenesis in vertebrates. In this study, a Chinese sturgeon Acipenser sinensis dead end homologue, designated Asdnd, was identified and characterized. The full-length cDNA of Asdnd was 1630 base pairs (bp) and encoded a peptide of 396 amino acid residues. Multiple sequence alignment showed that AsDnd shared six conserved regions of Dnd orthologs, including the RNA recognition motif. Phylogenetic analysis revealed that AsDnd was grouped with teleosts. By quantitative real-time PCR analysis, the Asdnd transcripts were found to originate from the maternal parent and be specifically expressed in gonads of immature Chinese sturgeons of both sexes. Fluorescent in situ hybridization indicated thatAsdnd transcripts were restricted to germ cells. In the testis, Asdnd was abundant in spermatogonia and tended to gradually diminish in late spermatogenic stages, while strong signals were found in primary oocytes, as opposed to oogonia, in the ovary. Zebrafish PGCs were clearly visualized at 24 h post-fertilization by co-injecting RFP-Asdnd 3' UTR and GFP-nos3 3' UTR mRNA, indicating that dnd 3' UTR has a conserved function among teleosts. Therefore, dnd could serve as a germ cell marker in Chinese sturgeon. (C) 2015 Elsevier B.V. All rights reserved.
关键词[WOS]: MESSENGER-RNA ; ZEBRAFISH MIR-430 ; EXPRESSION ; CLEARANCE ; MIGRATION ; SOMA
语种: 英语
WOS记录号: WOS:000349505100014
ISSN号: 0378-1119
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/23839
Appears in Collections:水生生物多样性与资源保护研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Univ Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Fishery Sci, Key Lab Freshwater Biodivers Conservat, Minist Agr China, Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China
3.Chinese Acad Fisheries Sci, Freshwater Fisheries Res Ctr, Wuxi 214081, Peoples R China
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文件名: Identification of a germ cell marker gene, the dead end homologue, in Chinese sturgeon Acipenser sinensis.pdf
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