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题名: Physicochemical Evolution and Molecular Adaptation of the Cetacean Osmoregulation-related Gene UT-A2 and Implications for Functional Studies
作者: Wang, Jingzhen1, 2, 3; Yu, Xueying1, 2, 4; Hu, Bo1, 2; Zheng, Jinsong1, 2; Xiao, Wuhan1, 2; Hao, Yujiang1, 2; Liu, Wenhua3; Wang, Ding1, 2
通讯作者: Liu, WH (reprint author), Shantou Univ, Inst Marine Biol, Shantou 515063, Guangdong, Peoples R China.
刊名: SCIENTIFIC REPORTS
发表日期: 2015-03-12
DOI: 10.1038/srep08795
卷: 5
收录类别: SCI
文章类型: Article
部门归属: [Wang, Jingzhen ; Yu, Xueying ; Hu, Bo ; Zheng, Jinsong ; Xiao, Wuhan ; Hao, Yujiang ; Wang, Ding] Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Hubei, Peoples R China ; [Wang, Jingzhen ; Yu, Xueying ; Hu, Bo ; Zheng, Jinsong ; Xiao, Wuhan ; Hao, Yujiang ; Wang, Ding] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China ; [Wang, Jingzhen ; Liu, Wenhua] Shantou Univ, Inst Marine Biol, Shantou 515063, Guangdong, Peoples R China ; [Yu, Xueying] Univ Chinese Acad Sci, Beijing 100039, Peoples R China
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
英文摘要: Cetaceans have an enigmatic evolutionary history of re-invading aquatic habitats. One of their essential adaptabilities that has enabled this process is their homeostatic strategy adjustment. Here, we investigated the physicochemical evolution and molecular adaptation of the cetacean urea transporter UT-A2, which plays an important role in urine concentration and water homeostasis. First, we cloned UT-A2 from the freshwater Yangtze finless porpoise, after which bioinformatics analyses were conducted based on available datasets (including freshwater baiji and marine toothed and baleen whales) using MEGA, PAML, DataMonkey, TreeSAAP and Consurf. Our findings suggest that the UT-A2 protein shows folding similar to that of dvUT and UT-B, whereas some variations occurred in the functional S-o and S-i regions of the selectivity filter. Additionally, several regions of the cetacean UT-A2 protein have experienced molecular adaptations. We suggest that positive-destabilizing selection could contribute to adaptations by influencing its biochemical and conformational character. The conservation of amino acid residues within the selectivity filter of the urea conduction pore is likely to be necessary for urea conduction, whereas the non-conserved amino acid replacements around the entrance and exit of the conduction pore could potentially affect the activity, which could be interesting target sites for future mutagenesis studies.
关键词[WOS]: REGULATED UREA TRANSPORTER ; AMINO-ACID SITES ; URINE-CONCENTRATING MECHANISM ; DOLPHINS TURSIOPS-TRUNCATUS ; GENOME-WIDE ASSOCIATION ; HELICOBACTER-PYLORI ; POSITIVE SELECTION ; MARINE MAMMALS ; RAT-KIDNEY ; ADAPTIVE EVOLUTION
语种: 英语
WOS记录号: WOS:000351142200001
ISSN号: 2045-2322
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/23835
Appears in Collections:水生生物多样性与资源保护研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
3.Shantou Univ, Inst Marine Biol, Shantou 515063, Guangdong, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
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