IHB OpenIR  > 水生生物多样性与资源保护研究中心  > 期刊论文
Physicochemical Evolution and Molecular Adaptation of the Cetacean Osmoregulation-related Gene UT-A2 and Implications for Functional Studies
Wang, Jingzhen1,2,3; Yu, Xueying1,2,4; Hu, Bo1,2; Zheng, Jinsong1,2; Xiao, Wuhan1,2; Hao, Yujiang1,2; Liu, Wenhua3; Wang, Ding1,2; Liu, WH (reprint author), Shantou Univ, Inst Marine Biol, Shantou 515063, Guangdong, Peoples R China.
2015-03-12
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
Volume5
AbstractCetaceans have an enigmatic evolutionary history of re-invading aquatic habitats. One of their essential adaptabilities that has enabled this process is their homeostatic strategy adjustment. Here, we investigated the physicochemical evolution and molecular adaptation of the cetacean urea transporter UT-A2, which plays an important role in urine concentration and water homeostasis. First, we cloned UT-A2 from the freshwater Yangtze finless porpoise, after which bioinformatics analyses were conducted based on available datasets (including freshwater baiji and marine toothed and baleen whales) using MEGA, PAML, DataMonkey, TreeSAAP and Consurf. Our findings suggest that the UT-A2 protein shows folding similar to that of dvUT and UT-B, whereas some variations occurred in the functional S-o and S-i regions of the selectivity filter. Additionally, several regions of the cetacean UT-A2 protein have experienced molecular adaptations. We suggest that positive-destabilizing selection could contribute to adaptations by influencing its biochemical and conformational character. The conservation of amino acid residues within the selectivity filter of the urea conduction pore is likely to be necessary for urea conduction, whereas the non-conserved amino acid replacements around the entrance and exit of the conduction pore could potentially affect the activity, which could be interesting target sites for future mutagenesis studies.
SubtypeArticle
Department[Wang, Jingzhen ; Yu, Xueying ; Hu, Bo ; Zheng, Jinsong ; Xiao, Wuhan ; Hao, Yujiang ; Wang, Ding] Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Hubei, Peoples R China ; [Wang, Jingzhen ; Yu, Xueying ; Hu, Bo ; Zheng, Jinsong ; Xiao, Wuhan ; Hao, Yujiang ; Wang, Ding] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China ; [Wang, Jingzhen ; Liu, Wenhua] Shantou Univ, Inst Marine Biol, Shantou 515063, Guangdong, Peoples R China ; [Yu, Xueying] Univ Chinese Acad Sci, Beijing 100039, Peoples R China
DOI10.1038/srep08795
WOS HeadingsScience & Technology
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000351142200001
WOS KeywordREGULATED UREA TRANSPORTER ; AMINO-ACID SITES ; URINE-CONCENTRATING MECHANISM ; DOLPHINS TURSIOPS-TRUNCATUS ; GENOME-WIDE ASSOCIATION ; HELICOBACTER-PYLORI ; POSITIVE SELECTION ; MARINE MAMMALS ; RAT-KIDNEY ; ADAPTIVE EVOLUTION
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23835
Collection水生生物多样性与资源保护研究中心_期刊论文
Corresponding AuthorLiu, WH (reprint author), Shantou Univ, Inst Marine Biol, Shantou 515063, Guangdong, Peoples R China.
Affiliation1.Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
3.Shantou Univ, Inst Marine Biol, Shantou 515063, Guangdong, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Wang, Jingzhen,Yu, Xueying,Hu, Bo,et al. Physicochemical Evolution and Molecular Adaptation of the Cetacean Osmoregulation-related Gene UT-A2 and Implications for Functional Studies[J]. SCIENTIFIC REPORTS,2015,5.
APA Wang, Jingzhen.,Yu, Xueying.,Hu, Bo.,Zheng, Jinsong.,Xiao, Wuhan.,...&Liu, WH .(2015).Physicochemical Evolution and Molecular Adaptation of the Cetacean Osmoregulation-related Gene UT-A2 and Implications for Functional Studies.SCIENTIFIC REPORTS,5.
MLA Wang, Jingzhen,et al."Physicochemical Evolution and Molecular Adaptation of the Cetacean Osmoregulation-related Gene UT-A2 and Implications for Functional Studies".SCIENTIFIC REPORTS 5(2015).
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