IHB OpenIR  > 水生生物多样性与资源保护研究中心  > 期刊论文
Differential expression of fertility genes boule and dazl in Chinese sturgeon (Acipenser sinensis), a basal fish
Ye, Huan1,2; Li, Chuang-Ju1,3; Yue, Hua-Mei1,3; Yang, Xiao-Ge1,2; Wei, Qi-Wei1,2,3; Wei, QW (reprint author), Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Minist Agr China, Key Lab Freshwater Biodivers Conservat,Donghu Hit, 8 1st Wudayuan Rd, Wuhan 430223, Peoples R China.
2015-05-01
Source PublicationCELL AND TISSUE RESEARCH
ISSN0302-766X
Volume360Issue:2Pages:413-425
AbstractThe gene family DAZ (deleted in Azoospermia), including boule, dazl and DAZ, performs highly conserved functions in germ cell development and fertility across animal phyla. Differential expression patterns have been demonstrated for the family members in invertebrates and vertebrates including fish. Here, we report the identification of boule and dazl and their expression at both RNA and protein levels in developing and mature gonads of Chinese sturgeon (Acipenser sinensis). Firstly, the isolation of the boule and dazl genes in Chinese sturgeon and the observation of the two genes in coelacanth suggest that dazl originated after the divergence of bony fish from cartilaginous fish but before the emergence of the Actinistia. Quantitative real-time PCR and western blot analyses reveal that boule and dazl RNA and proteins are restricted to the testis and ovary. In situ hybridization and fluorescent immunohistochemistry show that the bisexual mitotic and meiotic germ cell expression of dazl RNA and protein is conserved in vertebrates, while Chinese sturgeon boule RNA and protein exhibit mitotic and meiotic expression in the testis, and also likely display mitotic and meiotic expression in female. Moreover, we directly demonstrate for the first time that sturgeon Balbiani body/mitochondrial cloud disperses in the cytoplasm of early developing oocytes and co-localizes with Dazl to some extent. Finally, urbilaterian boule may also have an ancestral function in oogenesis. Taken together, these results provide useful information on the evolution of DAZ family genes, expression patterns and functions in animal reproduction.
SubtypeArticle
KeywordBoule Dazl Germ Cell Gametogenesis Chinese Sturgeon
Department[Ye, Huan ; Li, Chuang-Ju ; Yue, Hua-Mei ; Yang, Xiao-Ge ; Wei, Qi-Wei] Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Minist Agr China, Key Lab Freshwater Biodivers Conservat,Donghu Hit, Wuhan 430223, Peoples R China ; [Ye, Huan ; Yang, Xiao-Ge ; Wei, Qi-Wei] Chinese Acad Sci, Grad Univ, Inst Hydrobiol, Wuhan 430072, Peoples R China ; [Li, Chuang-Ju ; Yue, Hua-Mei ; Wei, Qi-Wei] Chinese Acad Fisheries Sci, Freshwater Fisheries Res Ctr, Wuxi 214081, Peoples R China
DOI10.1007/s00441-014-2095-2
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaCell Biology
WOS SubjectCell Biology
WOS IDWOS:000355567700018
WOS KeywordGERM-CELL DEVELOPMENT ; HUMAN Y-CHROMOSOME ; MITOCHONDRIAL CLOUD ; RNA LOCALIZATION ; PARTIAL RESCUE ; HOMOLOG ; XENOPUS ; GAMETOGENESIS ; PLASM ; MOUSE
Citation statistics
Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23820
Collection水生生物多样性与资源保护研究中心_期刊论文
Corresponding AuthorWei, QW (reprint author), Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Minist Agr China, Key Lab Freshwater Biodivers Conservat,Donghu Hit, 8 1st Wudayuan Rd, Wuhan 430223, Peoples R China.
Affiliation1.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Minist Agr China, Key Lab Freshwater Biodivers Conservat,Donghu Hit, Wuhan 430223, Peoples R China
2.Chinese Acad Sci, Grad Univ, Inst Hydrobiol, Wuhan 430072, Peoples R China
3.Chinese Acad Fisheries Sci, Freshwater Fisheries Res Ctr, Wuxi 214081, Peoples R China
Recommended Citation
GB/T 7714
Ye, Huan,Li, Chuang-Ju,Yue, Hua-Mei,et al. Differential expression of fertility genes boule and dazl in Chinese sturgeon (Acipenser sinensis), a basal fish[J]. CELL AND TISSUE RESEARCH,2015,360(2):413-425.
APA Ye, Huan,Li, Chuang-Ju,Yue, Hua-Mei,Yang, Xiao-Ge,Wei, Qi-Wei,&Wei, QW .(2015).Differential expression of fertility genes boule and dazl in Chinese sturgeon (Acipenser sinensis), a basal fish.CELL AND TISSUE RESEARCH,360(2),413-425.
MLA Ye, Huan,et al."Differential expression of fertility genes boule and dazl in Chinese sturgeon (Acipenser sinensis), a basal fish".CELL AND TISSUE RESEARCH 360.2(2015):413-425.
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