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Choreography of Transcriptomes and Lipidomes of Nannochloropsis Reveals the Mechanisms of Oil Synthesis in Microalgae
Li, Jing1,2,3; Han, Danxiang4; Wang, Dongmei1,2; Ning, Kang1,2; Jia, Jing1,2,3; Wei, Li1,2,3; Jing, Xiaoyan1,2; Huang, Shi1,2,3; Chen, Jie1,2; Li, Yantao5,6; Hu, Qiang7; Xu, Jian1,2
2014-04-01
Source PublicationPLANT CELL
ISSN1040-4651
Volume26Issue:4Pages:1645-1665
AbstractTo reveal the molecular mechanisms of oleaginousness in microalgae, transcriptomic and lipidomic dynamics of the oleaginous microalga Nannochloropsis oceanica IMET1 under nitrogen-replete (N+) and N-depleted (N-) conditions were simultaneously tracked. At the transcript level, enhanced triacylglycerol (TAG) synthesis under N-conditions primarily involved upregulation of seven putative diacylglycerol acyltransferase (DGAT) genes and downregulation of six other DGAT genes, with a simultaneous elevation of the other Kennedy pathway genes. Under N-conditions, despite downregulation of most de novo fatty acid synthesis genes, the pathways that shunt carbon precursors from protein and carbohydrate metabolic pathways into glycerolipid synthesis were stimulated at the transcript level. In particular, the genes involved in supplying carbon precursors and energy for de novo fatty acid synthesis, including those encoding components of the pyruvate dehydrogenase complex (PDHC), glycolysis, and PDHC bypass, and suites of specific transporters, were substantially upregulated under N-conditions, resulting in increased overall TAG production. Moreover, genes involved in the citric acid cycle and beta-oxidation in mitochondria were greatly enhanced to utilize the carbon skeletons derived from membrane lipids and proteins to produce additional TAG or its precursors. This temporal and spatial regulation model of oil accumulation in microalgae provides a basis for improving our understanding of TAG synthesis in microalgae and will also enable more rational genetic engineering of TAG production.
SubtypeArticle
DOI10.1105/tpc.113.121418
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
WOS SubjectBiochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
WOS IDWOS:000337351300021
WOS KeywordTANDEM MASS-SPECTROMETRY ; FATTY-ACID SYNTHESIS ; DIATOM CYCLOTELLA-CRYPTICA ; ACYL CARRIER PROTEIN ; CHLAMYDOMONAS-REINHARDTII ; TRIACYLGLYCEROL ACCUMULATION ; NITROGEN STARVATION ; ELECTROSPRAY-IONIZATION ; ARABIDOPSIS-THALIANA ; EXPRESSION ANALYSIS
Citation statistics
Cited Times:170[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23815
Collection藻类生物技术和生物能源研发中心_期刊论文
Affiliation1.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, CAS Key Lab Biofuels, Single Cell Ctr, Qingdao 266101, Shandong, Peoples R China
2.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao 266101, Shandong, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Arizona State Univ, Dept Appl Biol Sci, Lab Algae Res & Biotechnol, Mesa, AZ 85212 USA
5.Univ Maryland, Ctr Environm, Inst Marine & Environm Technol, Baltimore, MD 21202 USA
6.Univ Maryland Baltimore Cty, Baltimore, MD 21202 USA
7.Chinese Acad Sci, Inst Hydrobiol, Ctr Microalgal Biotechnol & Biofuels, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Li, Jing,Han, Danxiang,Wang, Dongmei,et al. Choreography of Transcriptomes and Lipidomes of Nannochloropsis Reveals the Mechanisms of Oil Synthesis in Microalgae[J]. PLANT CELL,2014,26(4):1645-1665.
APA Li, Jing.,Han, Danxiang.,Wang, Dongmei.,Ning, Kang.,Jia, Jing.,...&Xu, Jian.(2014).Choreography of Transcriptomes and Lipidomes of Nannochloropsis Reveals the Mechanisms of Oil Synthesis in Microalgae.PLANT CELL,26(4),1645-1665.
MLA Li, Jing,et al."Choreography of Transcriptomes and Lipidomes of Nannochloropsis Reveals the Mechanisms of Oil Synthesis in Microalgae".PLANT CELL 26.4(2014):1645-1665.
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