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题名: Comparative analyses of lipidomes and transcriptomes reveal a concerted action of multiple defensive systems against photooxidative stress in Haematococcus pluvialis
作者: Gwak, Yunho1; Hwang, Yong-sic2; Wang, Baobei3; Kim, Minju1; Jeong, Jooyeon1; Lee, Choul-Gyun4; Hu, Qiang5; Han, Danxiang3; Jin, EonSeon1
关键词: Astaxanthin ; high irradiance ; lipidome ; photooxidative stress ; transcriptome ; triacylglycerol
刊名: JOURNAL OF EXPERIMENTAL BOTANY
发表日期: 2014-08-01
DOI: 10.1093/jxb/eru206
卷: 65, 期:15, 页:4317-4334
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Plant Sciences
研究领域[WOS]: Plant Sciences
英文摘要: Haematococcus pluvialis cells predominantly remain in the macrozooid stage under favourable environmental conditions but are rapidly differentiated into haematocysts upon exposure to various environmental stresses. Haematocysts are characterized by massive accumulations of astaxanthin sequestered in cytosolic oil globules. Lipidomic analyses revealed that synthesis of the storage lipid triacylglycerol (TAG) was substantially stimulated under high irradiance. Simultaneously, remodelling of membrane glycerolipids occurred as a result of dramatic reductions in chloroplast membrane glycolipids but remained unchanged or declined slightly in extraplastidic membrane glycerolipids. De novo assembly of transcriptomes revealed the genomic and metabolic features of this unsequenced microalga. Comparative transcriptomic analysis showed that so-called resting cells (haematocysts) may be more active than fast-growing vegetative cells (macrozooids) regarding metabolic pathways and functions. Comparative transcriptomic analyses of astaxanthin biosynthesis suggested that the non-mevalonate pathway mediated the synthesis of isopentenyl diphosphate, as the majority of genes involved in subsequent astaxanthin biosynthesis were substantially up-regulated under high irradiance, with the genes encoding phytoene synthase, phytoene desaturase, and beta-carotene hydroxylase identified as the most prominent regulatory components. Accumulation of TAG under high irradiance was attributed to moderate up-regulation of de novo fatty acid biosynthesis at the gene level as well as to moderate elevation of the TAG assembly pathways. Additionally, inferred from transcriptomic differentiation, an increase in reactive oxygen species (ROS) scavenging activity, a decrease in ROS production, and the relaxation of over-reduction of the photosynthetic electron transport chain will work together to protect against photooxidative stress in H. pluvialis under high irradiance.
关键词[WOS]: CAROTENOID BIOSYNTHESIS GENES ; ALGA DUNALIELLA-BARDAWIL ; PLASTID TERMINAL OXIDASE ; ASTAXANTHIN BIOSYNTHESIS ; SECONDARY CAROTENOIDS ; CHLAMYDOMONAS-REINHARDTII ; PHYTOENE DESATURASE ; FATTY-ACIDS ; EXPRESSION ; ACCUMULATION
语种: 英语
WOS记录号: WOS:000339954300017
ISSN号: 0022-0957
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/23813
Appears in Collections:藻类生物技术和生物能源研发中心_期刊论文

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作者单位: 1.Hanyang Univ, Res Inst Nat Sci, Dept Life Sci, Seoul 133791, South Korea
2.Konkuk Univ, Dept Biosci & Biotechnol, Seoul 143701, South Korea
3.Arizona State Univ, Coll Technol & Innovat, Mesa, AZ 85212 USA
4.Inha Univ, Inst Ind Biotechnol, Dept Biotechnol, Inchon 402751, South Korea
5.Chinese Acad Sci, Inst Hydrobiol, Ctr Microalgal Biotechnol & Biofuels, Wuhan 430072, Hubei, Peoples R China
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文件名: Comparative analyses of lipidomes and transcriptomes reveal a concerted action of multiple defensive systems against photooxidative stress in Haematococcus pluvialis.pdf
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