IHB OpenIR  > 藻类生物技术和生物能源研发中心  > 期刊论文
Comparative analyses of lipidomes and transcriptomes reveal a concerted action of multiple defensive systems against photooxidative stress in Haematococcus pluvialis
Gwak, Yunho1; Hwang, Yong-sic2; Wang, Baobei3; Kim, Minju1; Jeong, Jooyeon1; Lee, Choul-Gyun4; Hu, Qiang5; Han, Danxiang3; Jin, EonSeon1
2014-08-01
Source PublicationJOURNAL OF EXPERIMENTAL BOTANY
ISSN0022-0957
Volume65Issue:15Pages:4317-4334
AbstractHaematococcus pluvialis cells predominantly remain in the macrozooid stage under favourable environmental conditions but are rapidly differentiated into haematocysts upon exposure to various environmental stresses. Haematocysts are characterized by massive accumulations of astaxanthin sequestered in cytosolic oil globules. Lipidomic analyses revealed that synthesis of the storage lipid triacylglycerol (TAG) was substantially stimulated under high irradiance. Simultaneously, remodelling of membrane glycerolipids occurred as a result of dramatic reductions in chloroplast membrane glycolipids but remained unchanged or declined slightly in extraplastidic membrane glycerolipids. De novo assembly of transcriptomes revealed the genomic and metabolic features of this unsequenced microalga. Comparative transcriptomic analysis showed that so-called resting cells (haematocysts) may be more active than fast-growing vegetative cells (macrozooids) regarding metabolic pathways and functions. Comparative transcriptomic analyses of astaxanthin biosynthesis suggested that the non-mevalonate pathway mediated the synthesis of isopentenyl diphosphate, as the majority of genes involved in subsequent astaxanthin biosynthesis were substantially up-regulated under high irradiance, with the genes encoding phytoene synthase, phytoene desaturase, and beta-carotene hydroxylase identified as the most prominent regulatory components. Accumulation of TAG under high irradiance was attributed to moderate up-regulation of de novo fatty acid biosynthesis at the gene level as well as to moderate elevation of the TAG assembly pathways. Additionally, inferred from transcriptomic differentiation, an increase in reactive oxygen species (ROS) scavenging activity, a decrease in ROS production, and the relaxation of over-reduction of the photosynthetic electron transport chain will work together to protect against photooxidative stress in H. pluvialis under high irradiance.
SubtypeArticle
KeywordAstaxanthin High Irradiance Lipidome Photooxidative Stress Transcriptome Triacylglycerol
DOI10.1093/jxb/eru206
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaPlant Sciences
WOS SubjectPlant Sciences
WOS IDWOS:000339954300017
WOS KeywordCAROTENOID BIOSYNTHESIS GENES ; ALGA DUNALIELLA-BARDAWIL ; PLASTID TERMINAL OXIDASE ; ASTAXANTHIN BIOSYNTHESIS ; SECONDARY CAROTENOIDS ; CHLAMYDOMONAS-REINHARDTII ; PHYTOENE DESATURASE ; FATTY-ACIDS ; EXPRESSION ; ACCUMULATION
Citation statistics
Cited Times:74[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23813
Collection藻类生物技术和生物能源研发中心_期刊论文
Affiliation1.Hanyang Univ, Res Inst Nat Sci, Dept Life Sci, Seoul 133791, South Korea
2.Konkuk Univ, Dept Biosci & Biotechnol, Seoul 143701, South Korea
3.Arizona State Univ, Coll Technol & Innovat, Mesa, AZ 85212 USA
4.Inha Univ, Inst Ind Biotechnol, Dept Biotechnol, Inchon 402751, South Korea
5.Chinese Acad Sci, Inst Hydrobiol, Ctr Microalgal Biotechnol & Biofuels, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Gwak, Yunho,Hwang, Yong-sic,Wang, Baobei,et al. Comparative analyses of lipidomes and transcriptomes reveal a concerted action of multiple defensive systems against photooxidative stress in Haematococcus pluvialis[J]. JOURNAL OF EXPERIMENTAL BOTANY,2014,65(15):4317-4334.
APA Gwak, Yunho.,Hwang, Yong-sic.,Wang, Baobei.,Kim, Minju.,Jeong, Jooyeon.,...&Jin, EonSeon.(2014).Comparative analyses of lipidomes and transcriptomes reveal a concerted action of multiple defensive systems against photooxidative stress in Haematococcus pluvialis.JOURNAL OF EXPERIMENTAL BOTANY,65(15),4317-4334.
MLA Gwak, Yunho,et al."Comparative analyses of lipidomes and transcriptomes reveal a concerted action of multiple defensive systems against photooxidative stress in Haematococcus pluvialis".JOURNAL OF EXPERIMENTAL BOTANY 65.15(2014):4317-4334.
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