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Cellular Capacities for High-Light Acclimation and Changing Lipid Profiles across Life Cycle Stages of the Green Alga Haematococcus pluvialis
Wang, Baobei1,2; Zhang, Zhen2,3; Hu, Qiang4; Sommerfeld, Milton2; Lu, Yinghua1; Han, Danxiang2
2014-09-15
Source PublicationPLOS ONE
ISSN1932-6203
Volume9Issue:9
AbstractThe unicellular microalga Haematococcus pluvialis has emerged as a promising biomass feedstock for the ketocarotenoid astaxanthin and neutral lipid triacylglycerol. Motile flagellates, resting palmella cells, and cysts are the major life cycle stages of H. pluvialis. Fast-growing motile cells are usually used to induce astaxanthin and triacylglycerol biosynthesis under stress conditions (high light or nutrient starvation); however, productivity of biomass and bioproducts are compromised due to the susceptibility of motile cells to stress. This study revealed that the Photosystem II (PSII) reaction center D1 protein, the manganese-stabilizing protein PsbO, and several major membrane glycerolipids (particularly for chloroplast membrane lipids monogalactosyldiacylglycerol and phosphatidylglycerol), decreased dramatically in motile cells under high light (HL). In contrast, palmella cells, which are transformed from motile cells after an extended period of time under favorable growth conditions, have developed multiple protective mechanisms-including reduction in chloroplast membrane lipids content, downplay of linear photosynthetic electron transport, and activating nonphotochemical quenching mechanisms-while accumulating triacylglycerol. Consequently, the membrane lipids and PSII proteins (D1 and PsbO) remained relatively stable in palmella cells subjected to HL. Introducing palmella instead of motile cells to stress conditions may greatly increase astaxanthin and lipid production in H. pluvialis culture.
SubtypeArticle
DOI10.1371/journal.pone.0106679
WOS HeadingsScience & Technology
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000341774800023
WOS KeywordTANDEM MASS-SPECTROMETRY ; PHOTOSYNTHETIC ELECTRON-TRANSPORT ; CHLAMYDOMONAS-REINHARDTII ; PHOTOSYSTEM-II ; GALACTOLIPID SYNTHESIS ; ASTAXANTHIN PRODUCTION ; PARIETOCHLORIS-INCISA ; THYLAKOID BIOGENESIS ; NITROGEN STARVATION ; ANGSTROM RESOLUTION
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Cited Times:19[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23812
Collection藻类生物技术和生物能源研发中心_期刊论文
Affiliation1.Xiamen Univ, Dept Chem & Biochem Engn, Xiamen, Fujian, Peoples R China
2.Arizona State Univ, Dept Human Syst & Environm, Mesa, AZ USA
3.E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
4.Chinese Acad Sci, Ctr Microalgal Biotechnol & Biofuels, Inst Hydrobiol, Wuhan, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Wang, Baobei,Zhang, Zhen,Hu, Qiang,et al. Cellular Capacities for High-Light Acclimation and Changing Lipid Profiles across Life Cycle Stages of the Green Alga Haematococcus pluvialis[J]. PLOS ONE,2014,9(9).
APA Wang, Baobei,Zhang, Zhen,Hu, Qiang,Sommerfeld, Milton,Lu, Yinghua,&Han, Danxiang.(2014).Cellular Capacities for High-Light Acclimation and Changing Lipid Profiles across Life Cycle Stages of the Green Alga Haematococcus pluvialis.PLOS ONE,9(9).
MLA Wang, Baobei,et al."Cellular Capacities for High-Light Acclimation and Changing Lipid Profiles across Life Cycle Stages of the Green Alga Haematococcus pluvialis".PLOS ONE 9.9(2014).
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