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Immunotoxicity Assessment of Rice-Derived Recombinant Human Serum Albumin Using Human Peripheral Blood Mononuclear Cells
Fu, Kai1,2; Cheng, Qin1,2; Liu, Zhenwei1,2; Chen, Zhen1,2; Wang, Yan4; Ruan, Honggang3; Zhou, Lu3; Xiong, Jie3; Xiao, Ruijing3; Liu, Shengwu3; Zhang, Qiuping3; Yang, Daichang1,2
2014-08-06
Source PublicationPLOS ONE
ISSN1932-6203
Volume9Issue:8
AbstractHuman serum albumin (HSA) is extensively used in clinics to treat a variety of diseases, such as hypoproteinemia, hemorrhagic shock, serious burn injuries, cirrhotic ascites and fetal erythroblastosis. To address supply shortages and high safety risks from limited human donors, we recently developed recombinant technology to produce HSA from rice endosperm. To assess he risk potential of HSA derived from Oryza sativa (OsrHSA) before a First-in-human (FIH) trial, we compared OsrHSA and plasma-derived HSA (pHSA), evaluating the potential for an immune reaction and toxicity using human peripheral blood mononuclear cells (PBMCs). The results indicated that neither OsrHSA nor pHSA stimulated T cell proliferation at 1x and 5x dosages. We also found no significant differences in the profiles of the CD4(+) and CD8(+) T cell subsets between OsrHSA- and pHSA-treated cells. Furthermore, the results showed that there were no significant differences between OsrHSA and pHSA in the production of cytokines such as interferon-gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-10 and IL-4. Our results demonstrated that OsrHSA has equivalent immunotoxicity to pHSA when using the PBMC model. Moreover, this ex vivo system could provide an alternative approach to predict potential risks in novel biopharmaceutical development.
SubtypeArticle
DOI10.1371/journal.pone.0104426
WOS HeadingsScience & Technology
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS KeywordMONOCLONAL-ANTIBODY TGN1412 ; PROTEIN O-GLYCOSYLATION ; SACCHAROMYCES-CEREVISIAE ; IMMUNE-RESPONSES ; CYTOKINE STORM ; EXPRESSION ; SECRETION ; SAFETY ; YEAST ; PURIFICATION
WOS IDWOS:000339995100070
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23809
Collection分析测试中心
Affiliation1.Wuhan Univ, State Key Lab Hybrid Rice, Wuhan 430072, Peoples R China
2.Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China
3.Wuhan Univ, Dept Immunol, Coll Basic Med Sci, Wuhan 430072, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, Anal & Testing Ctr, Wuhan, Peoples R China
Recommended Citation
GB/T 7714
Fu, Kai,Cheng, Qin,Liu, Zhenwei,et al. Immunotoxicity Assessment of Rice-Derived Recombinant Human Serum Albumin Using Human Peripheral Blood Mononuclear Cells[J]. PLOS ONE,2014,9(8).
APA Fu, Kai.,Cheng, Qin.,Liu, Zhenwei.,Chen, Zhen.,Wang, Yan.,...&Yang, Daichang.(2014).Immunotoxicity Assessment of Rice-Derived Recombinant Human Serum Albumin Using Human Peripheral Blood Mononuclear Cells.PLOS ONE,9(8).
MLA Fu, Kai,et al."Immunotoxicity Assessment of Rice-Derived Recombinant Human Serum Albumin Using Human Peripheral Blood Mononuclear Cells".PLOS ONE 9.8(2014).
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