中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 分析测试中心  > 期刊论文
题名: Immunotoxicity Assessment of Rice-Derived Recombinant Human Serum Albumin Using Human Peripheral Blood Mononuclear Cells
作者: Fu, Kai1, 2; Cheng, Qin1, 2; Liu, Zhenwei1, 2; Chen, Zhen1, 2; Wang, Yan4; Ruan, Honggang3; Zhou, Lu3; Xiong, Jie3; Xiao, Ruijing3; Liu, Shengwu3; Zhang, Qiuping3; Yang, Daichang1, 2
刊名: PLOS ONE
发表日期: 2014-08-06
DOI: 10.1371/journal.pone.0104426
卷: 9, 期:8
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
英文摘要: Human serum albumin (HSA) is extensively used in clinics to treat a variety of diseases, such as hypoproteinemia, hemorrhagic shock, serious burn injuries, cirrhotic ascites and fetal erythroblastosis. To address supply shortages and high safety risks from limited human donors, we recently developed recombinant technology to produce HSA from rice endosperm. To assess he risk potential of HSA derived from Oryza sativa (OsrHSA) before a First-in-human (FIH) trial, we compared OsrHSA and plasma-derived HSA (pHSA), evaluating the potential for an immune reaction and toxicity using human peripheral blood mononuclear cells (PBMCs). The results indicated that neither OsrHSA nor pHSA stimulated T cell proliferation at 1x and 5x dosages. We also found no significant differences in the profiles of the CD4(+) and CD8(+) T cell subsets between OsrHSA- and pHSA-treated cells. Furthermore, the results showed that there were no significant differences between OsrHSA and pHSA in the production of cytokines such as interferon-gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-10 and IL-4. Our results demonstrated that OsrHSA has equivalent immunotoxicity to pHSA when using the PBMC model. Moreover, this ex vivo system could provide an alternative approach to predict potential risks in novel biopharmaceutical development.
关键词[WOS]: MONOCLONAL-ANTIBODY TGN1412 ; PROTEIN O-GLYCOSYLATION ; SACCHAROMYCES-CEREVISIAE ; IMMUNE-RESPONSES ; CYTOKINE STORM ; EXPRESSION ; SECRETION ; SAFETY ; YEAST ; PURIFICATION
语种: 英语
WOS记录号: WOS:000339995100070
ISSN号: 1932-6203
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/23809
Appears in Collections:分析测试中心_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Immunotoxicity Assessment of Rice-Derived Recombinant Human Serum Albumin Using Human Peripheral Blood Mononuclear Cells.pdf(1133KB)----开放获取View Download

作者单位: 1.Wuhan Univ, State Key Lab Hybrid Rice, Wuhan 430072, Peoples R China
2.Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China
3.Wuhan Univ, Dept Immunol, Coll Basic Med Sci, Wuhan 430072, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, Anal & Testing Ctr, Wuhan, Peoples R China
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Fu, Kai]'s Articles
[Cheng, Qin]'s Articles
[Liu, Zhenwei]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Fu, Kai]‘s Articles
[Cheng, Qin]‘s Articles
[Liu, Zhenwei]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Immunotoxicity Assessment of Rice-Derived Recombinant Human Serum Albumin Using Human Peripheral Blood Mononuclear Cells.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2016  中国科学院水生生物研究所 - Feedback
Powered by CSpace