| Immunotoxicity Assessment of Rice-Derived Recombinant Human Serum Albumin Using Human Peripheral Blood Mononuclear Cells | |
| Fu, Kai1,2; Cheng, Qin1,2; Liu, Zhenwei1,2; Chen, Zhen1,2; Wang, Yan4; Ruan, Honggang3; Zhou, Lu3; Xiong, Jie3; Xiao, Ruijing3; Liu, Shengwu3; Zhang, Qiuping3; Yang, Daichang1,2 | |
| 2014-08-06 | |
| Source Publication | PLOS ONE
 |
| ISSN | 1932-6203 |
| Volume | 9Issue:8 |
| Abstract | Human serum albumin (HSA) is extensively used in clinics to treat a variety of diseases, such as hypoproteinemia, hemorrhagic shock, serious burn injuries, cirrhotic ascites and fetal erythroblastosis. To address supply shortages and high safety risks from limited human donors, we recently developed recombinant technology to produce HSA from rice endosperm. To assess he risk potential of HSA derived from Oryza sativa (OsrHSA) before a First-in-human (FIH) trial, we compared OsrHSA and plasma-derived HSA (pHSA), evaluating the potential for an immune reaction and toxicity using human peripheral blood mononuclear cells (PBMCs). The results indicated that neither OsrHSA nor pHSA stimulated T cell proliferation at 1x and 5x dosages. We also found no significant differences in the profiles of the CD4(+) and CD8(+) T cell subsets between OsrHSA- and pHSA-treated cells. Furthermore, the results showed that there were no significant differences between OsrHSA and pHSA in the production of cytokines such as interferon-gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-10 and IL-4. Our results demonstrated that OsrHSA has equivalent immunotoxicity to pHSA when using the PBMC model. Moreover, this ex vivo system could provide an alternative approach to predict potential risks in novel biopharmaceutical development. |
| Subtype | Article |
| DOI | 10.1371/journal.pone.0104426 |
| WOS Headings | Science & Technology |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Science & Technology - Other Topics |
| WOS Subject | Multidisciplinary Sciences |
| WOS ID | WOS:000339995100070 |
| WOS Keyword | MONOCLONAL-ANTIBODY TGN1412 ; PROTEIN O-GLYCOSYLATION ; SACCHAROMYCES-CEREVISIAE ; IMMUNE-RESPONSES ; CYTOKINE STORM ; EXPRESSION ; SECRETION ; SAFETY ; YEAST ; PURIFICATION |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/23809 |
| Collection | 分析测试中心_期刊论文 |
| Affiliation | 1.Wuhan Univ, State Key Lab Hybrid Rice, Wuhan 430072, Peoples R China 2.Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China 3.Wuhan Univ, Dept Immunol, Coll Basic Med Sci, Wuhan 430072, Peoples R China 4.Chinese Acad Sci, Inst Hydrobiol, Anal & Testing Ctr, Wuhan, Peoples R China |
| Recommended Citation GB/T 7714 | Fu, Kai,Cheng, Qin,Liu, Zhenwei,et al. Immunotoxicity Assessment of Rice-Derived Recombinant Human Serum Albumin Using Human Peripheral Blood Mononuclear Cells[J]. PLOS ONE,2014,9(8). |
| APA | Fu, Kai.,Cheng, Qin.,Liu, Zhenwei.,Chen, Zhen.,Wang, Yan.,...&Yang, Daichang.(2014).Immunotoxicity Assessment of Rice-Derived Recombinant Human Serum Albumin Using Human Peripheral Blood Mononuclear Cells.PLOS ONE,9(8). |
| MLA | Fu, Kai,et al."Immunotoxicity Assessment of Rice-Derived Recombinant Human Serum Albumin Using Human Peripheral Blood Mononuclear Cells".PLOS ONE 9.8(2014). |
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| Immunotoxicity Asses（1133KB） | 开放获取 | CC BY-NC-SA | View Application Full Text | |||
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