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Methylcrotonyl-CoA Carboxylase Regulates Triacylglycerol Accumulation in the Model Diatom Phaeodactylum tricornutum
Ge, Feng1; Huang, Weichao2; Chen, Zhuo2; Zhang, Chunye2; Xiong, Qian1; Bowler, Chris3; Yang, Juan2; Xu, Jin2; Hu, Hanhua1,2
2014-04-01
Source PublicationPLANT CELL
ISSN1040-4651
Volume26Issue:4Pages:1681-1697
AbstractThe model marine diatom Phaeodactylum tricornutum can accumulate high levels of triacylglycerols (TAGs) under nitrogen depletion and has attracted increasing attention as a potential system for biofuel production. However, the molecular mechanisms involved in TAG accumulation in diatoms are largely unknown. Here, we employed a label-free quantitative proteomics approach to estimate differences in protein abundance before and after TAG accumulation. We identified a total of 1193 proteins, 258 of which were significantly altered during TAG accumulation. Data analysis revealed major changes in proteins involved in branched-chain amino acid (BCAA) catabolic processes, glycolysis, and lipid metabolic processes. Subsequent quantitative RT-PCR and protein gel blot analysis confirmed that four genes associated with BCAA degradation were significantly upregulated at both the mRNA and protein levels during TAG accumulation. The most significantly upregulated gene, encoding the beta-subunit of methylcrotonyl-CoA carboxylase (MCC2), was selected for further functional studies. Inhibition of MCC2 expression by RNA interference disturbed the flux of carbon (mainly in the form of leucine) toward BCAA degradation, resulting in decreased TAG accumulation. MCC2 inhibition also gave rise to incomplete utilization of nitrogen, thus lowering biomass during the stationary growth phase. These findings help elucidate the molecular and metabolic mechanisms leading to increased lipid production in diatoms.
SubtypeArticle
KeywordMicroalga Chlamydomonas-reinhardtii Lipid-accumulation Nitrogen Deprivation Mass-spectrometry Oil Accumulation Brassica-napus Malic Enzyme Urea Cycle Metabolism Quantification
DOI10.1105/tpc.114.124982
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
WOS SubjectBiochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
WOS KeywordMICROALGA CHLAMYDOMONAS-REINHARDTII ; LIPID-ACCUMULATION ; NITROGEN DEPRIVATION ; MASS-SPECTROMETRY ; OIL ACCUMULATION ; BRASSICA-NAPUS ; MALIC ENZYME ; UREA CYCLE ; METABOLISM ; QUANTIFICATION
WOS IDWOS:000337351300023
Citation statistics
Cited Times:45[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20408
Collection藻类生物学及应用研究中心
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Diatom Biol Grp, Wuhan 430072, Peoples R China
3.Ecole Normale Super, CNRS, Unite Mixte Rech 8197,U1024, INSERM,Environm & Evolutionary Genom Sect,Inst Bi, F-75230 Paris 05, France
Recommended Citation
GB/T 7714
Ge, Feng,Huang, Weichao,Chen, Zhuo,et al. Methylcrotonyl-CoA Carboxylase Regulates Triacylglycerol Accumulation in the Model Diatom Phaeodactylum tricornutum[J]. PLANT CELL,2014,26(4):1681-1697.
APA Ge, Feng.,Huang, Weichao.,Chen, Zhuo.,Zhang, Chunye.,Xiong, Qian.,...&Hu, Hanhua.(2014).Methylcrotonyl-CoA Carboxylase Regulates Triacylglycerol Accumulation in the Model Diatom Phaeodactylum tricornutum.PLANT CELL,26(4),1681-1697.
MLA Ge, Feng,et al."Methylcrotonyl-CoA Carboxylase Regulates Triacylglycerol Accumulation in the Model Diatom Phaeodactylum tricornutum".PLANT CELL 26.4(2014):1681-1697.
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