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题名: Methylcrotonyl-CoA Carboxylase Regulates Triacylglycerol Accumulation in the Model Diatom Phaeodactylum tricornutum
作者: Ge, Feng1; Huang, Weichao2; Chen, Zhuo2; Zhang, Chunye2; Xiong, Qian1; Bowler, Chris3; Yang, Juan2; Xu, Jin2; Hu, Hanhua1, 2
关键词: MICROALGA CHLAMYDOMONAS-REINHARDTII ; LIPID-ACCUMULATION ; NITROGEN DEPRIVATION ; MASS-SPECTROMETRY ; OIL ACCUMULATION ; BRASSICA-NAPUS ; MALIC ENZYME ; UREA CYCLE ; METABOLISM ; QUANTIFICATION
刊名: PLANT CELL
发表日期: 2014-04-01
DOI: 10.1105/tpc.114.124982
卷: 26, 期:4, 页:1681-1697
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
英文摘要: The model marine diatom Phaeodactylum tricornutum can accumulate high levels of triacylglycerols (TAGs) under nitrogen depletion and has attracted increasing attention as a potential system for biofuel production. However, the molecular mechanisms involved in TAG accumulation in diatoms are largely unknown. Here, we employed a label-free quantitative proteomics approach to estimate differences in protein abundance before and after TAG accumulation. We identified a total of 1193 proteins, 258 of which were significantly altered during TAG accumulation. Data analysis revealed major changes in proteins involved in branched-chain amino acid (BCAA) catabolic processes, glycolysis, and lipid metabolic processes. Subsequent quantitative RT-PCR and protein gel blot analysis confirmed that four genes associated with BCAA degradation were significantly upregulated at both the mRNA and protein levels during TAG accumulation. The most significantly upregulated gene, encoding the beta-subunit of methylcrotonyl-CoA carboxylase (MCC2), was selected for further functional studies. Inhibition of MCC2 expression by RNA interference disturbed the flux of carbon (mainly in the form of leucine) toward BCAA degradation, resulting in decreased TAG accumulation. MCC2 inhibition also gave rise to incomplete utilization of nitrogen, thus lowering biomass during the stationary growth phase. These findings help elucidate the molecular and metabolic mechanisms leading to increased lipid production in diatoms.
关键词[WOS]: MICROALGA CHLAMYDOMONAS-REINHARDTII ; LIPID-ACCUMULATION ; NITROGEN DEPRIVATION ; MASS-SPECTROMETRY ; OIL ACCUMULATION ; BRASSICA-NAPUS ; MALIC ENZYME ; UREA CYCLE ; METABOLISM ; QUANTIFICATION
语种: 英语
WOS记录号: WOS:000337351300023
ISSN号: 1040-4651
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/20408
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Diatom Biol Grp, Wuhan 430072, Peoples R China
3.Ecole Normale Super, CNRS, Unite Mixte Rech 8197,U1024, INSERM,Environm & Evolutionary Genom Sect,Inst Bi, F-75230 Paris 05, France

Recommended Citation:
Ge, Feng; Huang, Weichao; Chen, Zhuo; Zhang, Chunye; Xiong, Qian; Bowler, Chris; Yang, Juan; Xu, Jin; Hu, Hanhua.Methylcrotonyl-CoA Carboxylase Regulates Triacylglycerol Accumulation in the Model Diatom Phaeodactylum tricornutum,PLANT CELL,2014,():
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