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题名: High-efficiency nuclear transformation of the oleaginous marine Nannochloropsis species using PCR product
作者: Li, Fengjuan1; Gao, Dawen1; Hu, Hanhua2
关键词: electroporation ; Nannochloropsis transformation ; cotransformation ; GUS
刊名: BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
发表日期: 2014-05-01
DOI: 10.1080/09168451.2014.905184
卷: 78, 期:5, 页:812-817
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]: Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Chemistry, Applied ; Food Science & Technology
研究领域[WOS]: Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Chemistry ; Food Science & Technology
英文摘要: Nannochloropsis are model species for investigating biofuel production by algae. To develop them into an integrated photons-to-fuel production platform, high efficiency transformation methods are necessary. Here, we obtained the beta-tubulin promoter regions of all recognized species of genus Nannochloropsis, and successfully transformed all five marine species by electroporation. In addition, the PCR amplified double stranded DNA fragments (PCR fragments) based transformation system was established in these Nannochloropsis species, which showed much higher transformation efficiency (10.7-61.2 x 10 (6), 1.5-13-fold) than that of linearized plasmid based transformation. The cotransformation of N. salina using a circular plasmid containing a non-selectable GUS gene and a PCR fragment containing only a selection marker cassette was also achieved and found to be very efficient (over 50%). This simple and highly efficient transformation protocol reported in our study provided a useful tool for gene functional analysis and genetic engineering of the oleaginous Nannochloropsis species.
关键词[WOS]: DIATOM PHAEODACTYLUM-TRICORNUTUM ; FATTY-ACID-COMPOSITION ; MICROALGAE ; GROWTH ; EUSTIGMATOPHYCEAE ; TRIACYLGLYCEROLS ; CHLAMYDOMONAS ; AQUACULTURE ; GENES
语种: 英语
WOS记录号: WOS:000339070600014
ISSN号: 0916-8451
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/20406
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.Harbin Inst Technol, State Key Lab Urban Water Resource & Environm, Harbin 150006, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Peoples R China

Recommended Citation:
Li, Fengjuan; Gao, Dawen; Hu, Hanhua.High-efficiency nuclear transformation of the oleaginous marine Nannochloropsis species using PCR product,BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,2014,():
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