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题名: High-efficiency nuclear transformation of the diatom Phaeodactylum tricornutum by electroporation
作者: Zhang, Chunye1, 2; Hu, Hanhua1
关键词: Electroporation ; Diatom transformation ; Cotransformation ; eGFP ; GUS
刊名: MARINE GENOMICS
发表日期: 2014-08-01
DOI: 10.1016/j.margen.2013.10.003
卷: 16, 页:63-66
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Genetics & Heredity ; Marine & Freshwater Biology
研究领域[WOS]: Genetics & Heredity ; Marine & Freshwater Biology
英文摘要: We established a high-efficiency nuclear transformation method for the diatom Phaeodactylum tricornutum using an electroporation system. Based on a universal electroporation protocol, the conditions for the introduction of exogenous DNA including electric field strength and plasmid form were optimized. Following optimization, the diatom cells could be transformed with exogenous gene easily, the maximum transformation frequency obtained was 2.8 x 10(-5) cells. The cotransformation of P. tricornutum with a non-selective GUS gene together with the selectable resistance gene has also been achieved using our new method and found to be very efficient (up to 60%). The electroporation procedure described in this article offers a number of advantages, including simplicity, general utility, low-cost and high efficiency. The described method also provides some clue for developing electroporation transformation system in other eukaryotic microalgae. (C) 2013 Elsevier B.V. All rights reserved.
关键词[WOS]: GENETIC-TRANSFORMATION ; CYLINDROTHECA-FUSIFORMIS ; REPORTER GENES ; BACILLARIOPHYCEAE ; ESTABLISHMENT ; PROMOTER ; MARKER ; SYSTEM
语种: 英语
WOS记录号: WOS:000343392100008
ISSN号: 1874-7787
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/20402
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
Zhang, Chunye; Hu, Hanhua.High-efficiency nuclear transformation of the diatom Phaeodactylum tricornutum by electroporation,MARINE GENOMICS,2014,():
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