| High-efficiency nuclear transformation of the diatom Phaeodactylum tricornutum by electroporation | |
| Zhang, Chunye1,2; Hu, Hanhua1 | |
| 2014-08-01 | |
| Source Publication | MARINE GENOMICS
 |
| ISSN | 1874-7787 |
| Volume | 16Pages:63-66 |
| Abstract | We established a high-efficiency nuclear transformation method for the diatom Phaeodactylum tricornutum using an electroporation system. Based on a universal electroporation protocol, the conditions for the introduction of exogenous DNA including electric field strength and plasmid form were optimized. Following optimization, the diatom cells could be transformed with exogenous gene easily, the maximum transformation frequency obtained was 2.8 x 10(-5) cells. The cotransformation of P. tricornutum with a non-selective GUS gene together with the selectable resistance gene has also been achieved using our new method and found to be very efficient (up to 60%). The electroporation procedure described in this article offers a number of advantages, including simplicity, general utility, low-cost and high efficiency. The described method also provides some clue for developing electroporation transformation system in other eukaryotic microalgae. (C) 2013 Elsevier B.V. All rights reserved. |
| Subtype | Article |
| Keyword | Electroporation Diatom Transformation Cotransformation Egfp Gus |
| DOI | 10.1016/j.margen.2013.10.003 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Genetics & Heredity ; Marine & Freshwater Biology |
| WOS Subject | Genetics & Heredity ; Marine & Freshwater Biology |
| WOS ID | WOS:000343392100008 |
| WOS Keyword | GENETIC-TRANSFORMATION ; CYLINDROTHECA-FUSIFORMIS ; REPORTER GENES ; BACILLARIOPHYCEAE ; ESTABLISHMENT ; PROMOTER ; MARKER ; SYSTEM |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/20402 |
| Collection | 藻类生物学及应用研究中心_期刊论文 |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| Recommended Citation GB/T 7714 | Zhang, Chunye,Hu, Hanhua. High-efficiency nuclear transformation of the diatom Phaeodactylum tricornutum by electroporation[J]. MARINE GENOMICS,2014,16:63-66. |
| APA | Zhang, Chunye,&Hu, Hanhua.(2014).High-efficiency nuclear transformation of the diatom Phaeodactylum tricornutum by electroporation.MARINE GENOMICS,16,63-66. |
| MLA | Zhang, Chunye,et al."High-efficiency nuclear transformation of the diatom Phaeodactylum tricornutum by electroporation".MARINE GENOMICS 16(2014):63-66. |
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| File Name/Size | DocType | Version | Access | License | ||
| High-efficiency nucl（548KB） | 开放获取 | CC BY-NC-SA | View Application Full Text | |||
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