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High-efficiency nuclear transformation of the diatom Phaeodactylum tricornutum by electroporation
Zhang, Chunye1,2; Hu, Hanhua1
2014-08-01
Source PublicationMARINE GENOMICS
ISSN1874-7787
Volume16Pages:63-66
AbstractWe established a high-efficiency nuclear transformation method for the diatom Phaeodactylum tricornutum using an electroporation system. Based on a universal electroporation protocol, the conditions for the introduction of exogenous DNA including electric field strength and plasmid form were optimized. Following optimization, the diatom cells could be transformed with exogenous gene easily, the maximum transformation frequency obtained was 2.8 x 10(-5) cells. The cotransformation of P. tricornutum with a non-selective GUS gene together with the selectable resistance gene has also been achieved using our new method and found to be very efficient (up to 60%). The electroporation procedure described in this article offers a number of advantages, including simplicity, general utility, low-cost and high efficiency. The described method also provides some clue for developing electroporation transformation system in other eukaryotic microalgae. (C) 2013 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordElectroporation Diatom Transformation Cotransformation Egfp Gus
DOI10.1016/j.margen.2013.10.003
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaGenetics & Heredity ; Marine & Freshwater Biology
WOS SubjectGenetics & Heredity ; Marine & Freshwater Biology
WOS IDWOS:000343392100008
WOS KeywordGENETIC-TRANSFORMATION ; CYLINDROTHECA-FUSIFORMIS ; REPORTER GENES ; BACILLARIOPHYCEAE ; ESTABLISHMENT ; PROMOTER ; MARKER ; SYSTEM
Citation statistics
Cited Times:48[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20402
Collection藻类生物学及应用研究中心_期刊论文
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Chunye,Hu, Hanhua. High-efficiency nuclear transformation of the diatom Phaeodactylum tricornutum by electroporation[J]. MARINE GENOMICS,2014,16:63-66.
APA Zhang, Chunye,&Hu, Hanhua.(2014).High-efficiency nuclear transformation of the diatom Phaeodactylum tricornutum by electroporation.MARINE GENOMICS,16,63-66.
MLA Zhang, Chunye,et al."High-efficiency nuclear transformation of the diatom Phaeodactylum tricornutum by electroporation".MARINE GENOMICS 16(2014):63-66.
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