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Growth and Nitrogen Uptake Characteristics Reveal Outbreak Mechanism of the Opportunistic Macroalga Gracilaria tenuistipitata
Wang, Chao1,2; Lei, Anping1; Zhou, Kai3; Hu, Zhengyu2; Hao, Wenlong1; Yang, Junda1
2014-10-09
Source PublicationPLOS ONE
ISSN1932-6203
Volume9Issue:10
AbstractMacroalgae has bloomed in the brackish lake of Shenzhen Bay, China continuously from 2010 to 2014. Gracilaria tenuistipitata was identified as the causative macroalgal species. The aim of this study was to explore the outbreak mechanism of G. tenuistipitata, by studying the effects of salinity and nitrogen sources on growth, and the different nitrogen sources uptake characteristic. Our experimental design was based on environmental conditions observed in the bloom areas, and these main factors were simulated in the laboratory. Results showed that salinity 12 to 20 % was suitable for G. tenuistipitata growth. When the nitrogen sources' (NH4+, NO3+) concentrations reached 40 mu M or above, the growth rate of G. tenuistipitata was significantly higher. Algal biomass was higher (approximately 1.4 times) when cultured with NH4+ than that with NO3+ addition. Coincidentally, macroalgal bloom formed during times of moderate salinity (similar to 12 %) and high nitrogen conditions. The NH4+ and NO3+ uptake characteristic was studied to understand the potential mechanism of G. tenuistipitata bloom. NH4+ uptake was best described by a linear, rate-unsaturated response, with the slope decreasing with time intervals. In contrast, NO3+ uptake followed a rate-saturating mechanism best described by the Michaelis-Menten model, with kinetic parameters V-max = 37.2 mu M g(-1) DM h(-1) and K-s = 61.5 mu M. Further, based on the isotope N-15 tracer method, we found that N-15 from NH4+ accumulated faster and reached an atom% twice than that of N-15 from NO3+, suggesting when both NH4+ and NO3+ were available, NH4+ was assimilated more rapidly. The results of the present study indicate that in the estuarine environment, the combination of moderate salinity with high ammonium may stimulate bloom formation.
SubtypeArticle
KeywordNitrate Uptake Inorganic Nitrogen Coastal Waters Ulva-lactuca Intertidal Seaweeds Nutrient Control Ammonium Uptake Uptake Kinetics Agar Yield J. Agardh
DOI10.1371/journal.pone.0108980
WOS HeadingsScience & Technology
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000343941200027
WOS KeywordNITRATE UPTAKE ; INORGANIC NITROGEN ; COASTAL WATERS ; ULVA-LACTUCA ; INTERTIDAL SEAWEEDS ; NUTRIENT CONTROL ; AMMONIUM UPTAKE ; UPTAKE KINETICS ; AGAR YIELD ; J. AGARDH
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Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20394
Collection藻类生物学及应用研究中心_期刊论文
Affiliation1.Shenzhen Univ, Coll Life Sci, Shenzhen, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
3.Shenzhen Marine Environm & Resources Monitoring C, Shenzhen, Peoples R China
Recommended Citation
GB/T 7714
Wang, Chao,Lei, Anping,Zhou, Kai,et al. Growth and Nitrogen Uptake Characteristics Reveal Outbreak Mechanism of the Opportunistic Macroalga Gracilaria tenuistipitata[J]. PLOS ONE,2014,9(10).
APA Wang, Chao,Lei, Anping,Zhou, Kai,Hu, Zhengyu,Hao, Wenlong,&Yang, Junda.(2014).Growth and Nitrogen Uptake Characteristics Reveal Outbreak Mechanism of the Opportunistic Macroalga Gracilaria tenuistipitata.PLOS ONE,9(10).
MLA Wang, Chao,et al."Growth and Nitrogen Uptake Characteristics Reveal Outbreak Mechanism of the Opportunistic Macroalga Gracilaria tenuistipitata".PLOS ONE 9.10(2014).
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