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Hyperplasia and Cellularity Changes in IGF-1-Overexpressing Skeletal Muscle of Crucian Carp
Li, Dongliang1,2; Lou, Qiyong1; Zhai, Gang1,2; Peng, Xuyan1,2; Cheng, Xiaoxia1,2; Dai, Xiangyan1,2; Zhuo, Zijian1,2; Shang, Guohui1,2; Jin, Xia1; Chen, Xiaowen1; Han, Dong1; He, Jiangyan1; Yin, Zhan1
2014-06-01
Source PublicationENDOCRINOLOGY
ISSN0013-7227
Volume155Issue:6Pages:2199-2212
AbstractThe zebrafish skeletal muscle-specific promoter mylz2 was used to cause crucian carp overexpression of the zebrafish IGF-1 cDNA. In stable transgenic germline F-1 progenies, a 5-fold increase in the level of IGF-1 in skeletal muscle was observed. Evident skeletal muscle hyperplasia was observed in the transgenic fish through histologic analysis. By analyzing the RNA sequencing transcriptome of the skeletal muscle of IGF-1 transgenic fish and nontransgenic control fish at 15 months of age, 10 966 transcripts with significant expression levels were identified with definite gene descriptions based on the corresponding zebrafish genome information. Based on the results of our RNA sequencing transcriptome profiling analysis and the results of the real-time quantitative PCR analysis performed to confirm the skeletal muscle transcriptomics analysis, several pathways, including IGF-1 signaling, aerobic metabolism, and protein degradation, were found to be activated in the IGF-1-overexpressing transgenic fish. Intriguingly, our transcriptional expression and protein assays indicated that the overexpression of IGF-1 stimulated a significant shift in the myofiber type toward a more oxidative slow muscle type. Although the body weight was surprisingly decreased by IGF-1 transgenic expression, significantly higher oxygen consumption rates were measured in IGF-1-overexpressing transgenic fish compared with their nontransgenic control fish. These results indicate that the sustained overexpression of IGF-1 in crucian carp skeletal muscle promotes myofiber hyperplasia and cellularity changes, which elicit alterations in the body energy metabolism and skeletal muscle growth.
SubtypeArticle
KeywordGrowth-factor-i Rainbow-trout Igf-i Cell Differentiation Transgenic Zebrafish Atlantic Salmon Fish-meal Insulin Expression Metabolism
DOI10.1210/en.2013-1938
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaEndocrinology & Metabolism
WOS SubjectEndocrinology & Metabolism
WOS IDWOS:000342342900015
WOS KeywordGROWTH-FACTOR-I ; RAINBOW-TROUT ; IGF-I ; CELL DIFFERENTIATION ; TRANSGENIC ZEBRAFISH ; ATLANTIC SALMON ; FISH-MEAL ; INSULIN ; EXPRESSION ; METABOLISM
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Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20388
Collection水生生物分子与细胞生物学研究中心_期刊论文
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Li, Dongliang,Lou, Qiyong,Zhai, Gang,et al. Hyperplasia and Cellularity Changes in IGF-1-Overexpressing Skeletal Muscle of Crucian Carp[J]. ENDOCRINOLOGY,2014,155(6):2199-2212.
APA Li, Dongliang.,Lou, Qiyong.,Zhai, Gang.,Peng, Xuyan.,Cheng, Xiaoxia.,...&Yin, Zhan.(2014).Hyperplasia and Cellularity Changes in IGF-1-Overexpressing Skeletal Muscle of Crucian Carp.ENDOCRINOLOGY,155(6),2199-2212.
MLA Li, Dongliang,et al."Hyperplasia and Cellularity Changes in IGF-1-Overexpressing Skeletal Muscle of Crucian Carp".ENDOCRINOLOGY 155.6(2014):2199-2212.
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