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题名: Cd-Metallothioneins in Three Additional Tetrahymena Species: Intragenic Repeat Patterns and Induction by Metal Ions
作者: Chang, Yue1, 2; Liu, Guanglong3; Guo, Lina1; Liu, Hongbo1; Yuan, Dongxia1; Xiong, Jie1; Ning, Yingzhi3; Fu, Chengjie1, 4; Miao, Wei1
关键词: ciliate ; heavy metal stress ; metallothionein evolution ; modular repeat ; real-time quantitative PCR
刊名: JOURNAL OF EUKARYOTIC MICROBIOLOGY
发表日期: 2014-07-01
DOI: 10.1111/jeu.12112
卷: 61, 期:4, 页:333-342
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Microbiology
研究领域[WOS]: Microbiology
英文摘要: Ciliate metallothioneins (MTs) possess many unique features compared to the "classic" MTs in other organisms, but they have only been studied in a small number of species. In this study, we investigated cDNAs encoding subfamily 7a metallothioneins (CdMTs) in three Tetrahymena species (T. hegewischi, T. malaccensis, and T. mobilis). Four CdMT genes (ThegMT1, ThegMT2, TmalMT1, and TmobMT1) were cloned and characterized. They share high sequence similarity to previously identified subfamily 7a MT members. Tetrahymena CdMTs exhibit a remarkably regular intragenic repeat homology. The CdMT sequences were divided into two main types of modules, which had been previously described, and which we name "A" and "B". ThegMT2 was identified as the first MT isoform solely composed of module "B". A phylogenetic analysis of individual modules of every characterized Tetrahymena CdMT rigorously documents the conclusion that modules are important units of CdMT evolution, which have undergone frequent and rapid gain/loss and shuffling. The transcriptional activity of the four newly identified genes was measured under different heavy metal exposure (Cd, Cu, Zn, Pb) using real-time quantitative PCR. The results showed that these genes were differentially induced after short (1 h) or long (24 h) metal exposure. The evolutionary diversity of Tetrahymena CdMTs is further discussed with regard to their induction by metal ions.
关键词[WOS]: STRESS-INDUCIBLE METALLOTHIONEIN ; GENE-EXPRESSION ANALYSIS ; TROPICALIS-LAHORENSIS ; THERMOPHILA ; CLONING ; PYRIFORMIS ; PIGMENTOSA ; CILIATE ; IDENTIFICATION ; BIOSENSORS
语种: 英语
WOS记录号: WOS:000339115300001
ISSN号: 1066-5234
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/20386
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.Northwest Normal Sch, Coll Life Sci, Lanzhou 730070, Peoples R China
4.Uppsala Univ, Program Systemat Biol, Evolutionary Biol Ctr, S-75236 Uppsala, Sweden
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