IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Cd-Metallothioneins in Three Additional Tetrahymena Species: Intragenic Repeat Patterns and Induction by Metal Ions
Chang, Yue1,2; Liu, Guanglong3; Guo, Lina1; Liu, Hongbo1; Yuan, Dongxia1; Xiong, Jie1; Ning, Yingzhi3; Fu, Chengjie1,4; Miao, Wei1
2014-07-01
Source PublicationJOURNAL OF EUKARYOTIC MICROBIOLOGY
ISSN1066-5234
Volume61Issue:4Pages:333-342
AbstractCiliate metallothioneins (MTs) possess many unique features compared to the "classic" MTs in other organisms, but they have only been studied in a small number of species. In this study, we investigated cDNAs encoding subfamily 7a metallothioneins (CdMTs) in three Tetrahymena species (T. hegewischi, T. malaccensis, and T. mobilis). Four CdMT genes (ThegMT1, ThegMT2, TmalMT1, and TmobMT1) were cloned and characterized. They share high sequence similarity to previously identified subfamily 7a MT members. Tetrahymena CdMTs exhibit a remarkably regular intragenic repeat homology. The CdMT sequences were divided into two main types of modules, which had been previously described, and which we name "A" and "B". ThegMT2 was identified as the first MT isoform solely composed of module "B". A phylogenetic analysis of individual modules of every characterized Tetrahymena CdMT rigorously documents the conclusion that modules are important units of CdMT evolution, which have undergone frequent and rapid gain/loss and shuffling. The transcriptional activity of the four newly identified genes was measured under different heavy metal exposure (Cd, Cu, Zn, Pb) using real-time quantitative PCR. The results showed that these genes were differentially induced after short (1 h) or long (24 h) metal exposure. The evolutionary diversity of Tetrahymena CdMTs is further discussed with regard to their induction by metal ions.
SubtypeArticle
KeywordCiliate Heavy Metal Stress Metallothionein Evolution Modular Repeat Real-time Quantitative Pcr
DOI10.1111/jeu.12112
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000339115300001
WOS KeywordSTRESS-INDUCIBLE METALLOTHIONEIN ; GENE-EXPRESSION ANALYSIS ; TROPICALIS-LAHORENSIS ; THERMOPHILA ; CLONING ; PYRIFORMIS ; PIGMENTOSA ; CILIATE ; IDENTIFICATION ; BIOSENSORS
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20386
Collection水生生物分子与细胞生物学研究中心_期刊论文
Affiliation1.Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.Northwest Normal Sch, Coll Life Sci, Lanzhou 730070, Peoples R China
4.Uppsala Univ, Program Systemat Biol, Evolutionary Biol Ctr, S-75236 Uppsala, Sweden
Recommended Citation
GB/T 7714
Chang, Yue,Liu, Guanglong,Guo, Lina,et al. Cd-Metallothioneins in Three Additional Tetrahymena Species: Intragenic Repeat Patterns and Induction by Metal Ions[J]. JOURNAL OF EUKARYOTIC MICROBIOLOGY,2014,61(4):333-342.
APA Chang, Yue.,Liu, Guanglong.,Guo, Lina.,Liu, Hongbo.,Yuan, Dongxia.,...&Miao, Wei.(2014).Cd-Metallothioneins in Three Additional Tetrahymena Species: Intragenic Repeat Patterns and Induction by Metal Ions.JOURNAL OF EUKARYOTIC MICROBIOLOGY,61(4),333-342.
MLA Chang, Yue,et al."Cd-Metallothioneins in Three Additional Tetrahymena Species: Intragenic Repeat Patterns and Induction by Metal Ions".JOURNAL OF EUKARYOTIC MICROBIOLOGY 61.4(2014):333-342.
Files in This Item:
File Name/Size DocType Version Access License
Cd-Metallothioneins (1396KB) 开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Chang, Yue]'s Articles
[Liu, Guanglong]'s Articles
[Guo, Lina]'s Articles
Baidu academic
Similar articles in Baidu academic
[Chang, Yue]'s Articles
[Liu, Guanglong]'s Articles
[Guo, Lina]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Chang, Yue]'s Articles
[Liu, Guanglong]'s Articles
[Guo, Lina]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Cd-Metallothioneins in Three Additional Tetrahymena Species_ Intragenic Repeat Patterns and Induction by Metal Ions.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.