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IFN Regulatory Factor 10 Is a Negative Regulator of the IFN Responses in Fish
Li, Shun; Lu, Long-Feng; Feng, Hong; Wu, Nan; Chen, Dan-Dan; Zhang, Yi-Bing; Gui, Jian-Fang; Nie, Pin; Zhang, Yong-An
2014-08-01
Source PublicationJOURNAL OF IMMUNOLOGY
ISSN0022-1767
Volume193Issue:3Pages:1100-1109
AbstractIFN regulatory factor (IRF) 10 belongs to the IRF family and exists exclusively in birds and fish. Most IRFs have been identified as critical regulators in the IFN responses in both fish and mammals; however, the role of IRF10 is unclear. In this study, we identified IRF10 in zebrafish (Danio rerio) and found that it serves as a negative regulator to balance the innate antiviral immune responses. Zebrafish IRF10 (DrIRF10) was induced by intracellular polyinosinic:polycytidylic acid in ZF4 (zebrafish embryo fibroblast-like) cells. DrIRF10 inhibited the activation of zebrafish IFN1 (DrIFN1) and DrIFN3 promoters in epithelioma papulosum cyprinid cells in the presence or absence of polyinosinic:polycytidylic acid stimulation through direct interaction with the IFN promoters, and this inhibition was also shown to block IFN signaling. Overexpression of DrIRF10 was able to abolish the induction of DrIFN1 and DrIFN3 mediated by the retinoic acid inducible gene I like receptors. In addition, functional domain analysis of DrIRF10 showed that either the DNA binding domain or the IRF association domain is sufficient for its inhibitory activity for IFN signaling. Lastly, overexpression of DrIRF10 decreased the transcription level of several IFN-stimulated genes, resulting in the susceptibility of host cells to spring viremia of carp virus infection. Collectively, these data suggest that DrIRF10 inhibits the expression of DrIFN1 and DrIFN3 to avoid an excessive immune response, a unique regulation mechanism of the IFN responses in lower vertebrates.
SubtypeArticle
KeywordInnate Immune-response Interferon Antiviral Response i Interferon Rig-i Oncorhynchus-mykiss Atlantic Salmon Rainbow-trout Factor Irf Functional-characterization Feedback-regulation
DOI10.4049/jimmunol.1400253
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaImmunology
WOS SubjectImmunology
WOS IDWOS:000339422000016
WOS KeywordINNATE IMMUNE-RESPONSE ; INTERFERON ANTIVIRAL RESPONSE ; I INTERFERON ; RIG-I ; ONCORHYNCHUS-MYKISS ; ATLANTIC SALMON ; RAINBOW-TROUT ; FACTOR IRF ; FUNCTIONAL-CHARACTERIZATION ; FEEDBACK-REGULATION
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Cited Times:49[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20385
Collection水生生物分子与细胞生物学研究中心_期刊论文
AffiliationChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Li, Shun,Lu, Long-Feng,Feng, Hong,et al. IFN Regulatory Factor 10 Is a Negative Regulator of the IFN Responses in Fish[J]. JOURNAL OF IMMUNOLOGY,2014,193(3):1100-1109.
APA Li, Shun.,Lu, Long-Feng.,Feng, Hong.,Wu, Nan.,Chen, Dan-Dan.,...&Zhang, Yong-An.(2014).IFN Regulatory Factor 10 Is a Negative Regulator of the IFN Responses in Fish.JOURNAL OF IMMUNOLOGY,193(3),1100-1109.
MLA Li, Shun,et al."IFN Regulatory Factor 10 Is a Negative Regulator of the IFN Responses in Fish".JOURNAL OF IMMUNOLOGY 193.3(2014):1100-1109.
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