IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Dietary tryptophan modulates intestinal immune response, barrier function, antioxidant status and gene expression of TOR and Nrf2 in young grass carp (Ctenopharyngodon idella)
Wen, Hailang1; Feng, Lin1,2,3; Jiang, Weidan1,2,3; Liu, Yang1,2,3; Jiang, Jun1,2,3; Li, Shuhong1; Tang, Ling4; Zhang, Yongan5; Kuang, Shengyao4; Zhou, Xiaoqiu1,2,3
2014-09-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume40Issue:1Pages:275-287
AbstractThe present research evaluated the effects of dietary tryptophan (Trp) on growth performance, intestinal mucosa] immune, barrier function and antioxidant capacity and gene expression of young grass carp (Ctenophatyngodon idella). Fish were fed six different experimental diets containing graded levels of Trp at 0.7(control), 1.7, 3.1, 4.0, 52 and 6.1 g kg(-1) diet for 8 weeks. The results showed that Trp supplementation significantly enhanced the percent weight gain (PWG), feed intake and feed efficiency (P < 0.05), and decreased the plasma ammonia content (PAC) (P < 0.05). After the 8-week feeding trail, an environmental copper exposure trail was conducted for 4 days. Results from the copper exposure trail showed that dietary Trp enhanced the lysozyme, acid phosphatase activities and complement 3 contents in the intestine of young grass carp (P < 0.05). In addition, Trp supplementation increased the copper/zinc superoxide dismutase (SOD1), glutathione peroxidase (GPx) activities and glutathione contents (P < 0.05), and decreased the protein carbonyl and malondialdehyde contents (P < 0.05). Furthermore, the relative gene expression levels of interleukin 10, transforming growth factor-beta 1, occludin, zonula occludens 1, claudin-b, -c, and 3, SOD1, GPx and NF-E2-related factor 2 in the intestine were significantly up-regulated with increasing of dietary Trp up to a certain level (P < 0.05). Conversely, the mRNA levels of tumor necrosis factor a, interleukin 8, target of rapamycin, Kelch-like-ECH-associated protein 1, claudin-12 and -15a in the intestine were significantly down-regulated by Trp (P < 0.05). Collectively, appropriate dietary Trp level improves fish growth, intestinal immune response, barrier function and antioxidant status, and regulated the mRNA levels of related signal molecules of young grass carp. Based on the quadratic regression analysis of the PWG and PAC, the dietary Trp requirement of young grass carp (287-699 g) was estimated to be 3.81 g kg(-1) diet (12.7 g kg(-1) protein) and 3.89 g kg(-1) diet (13.0 g kg(-1) protein), respectively. (C) 2014 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordTryptophan Immune Response Tight Junction Protein Antioxidant Status Nrf2
DOI10.1016/j.fsi.2014.07.004
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000341468500033
WOS KeywordSALMON SALMO-SALAR ; TROUT ONCORHYNCHUS-MYKISS ; INDUCED OXIDATIVE DAMAGE ; JUVENILE JIAN ; VAR. JIAN ; ENZYME-ACTIVITIES ; GROWTH-PERFORMANCE ; BODY-COMPOSITION ; PLASMA-CORTISOL ; MESSENGER-RNA
Citation statistics
Cited Times:82[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20384
Collection水生生物分子与细胞生物学研究中心_期刊论文
Affiliation1.Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China
2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov, Chengdu 611130, Sichuan, Peoples R China
3.Sichuan Agr Univ, China Minist Educ, Key Lab Anim Dis Resistance Nutr, Chengdu 611130, Sichuan, Peoples R China
4.Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 610066, Peoples R China
5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Wen, Hailang,Feng, Lin,Jiang, Weidan,et al. Dietary tryptophan modulates intestinal immune response, barrier function, antioxidant status and gene expression of TOR and Nrf2 in young grass carp (Ctenopharyngodon idella)[J]. FISH & SHELLFISH IMMUNOLOGY,2014,40(1):275-287.
APA Wen, Hailang.,Feng, Lin.,Jiang, Weidan.,Liu, Yang.,Jiang, Jun.,...&Zhou, Xiaoqiu.(2014).Dietary tryptophan modulates intestinal immune response, barrier function, antioxidant status and gene expression of TOR and Nrf2 in young grass carp (Ctenopharyngodon idella).FISH & SHELLFISH IMMUNOLOGY,40(1),275-287.
MLA Wen, Hailang,et al."Dietary tryptophan modulates intestinal immune response, barrier function, antioxidant status and gene expression of TOR and Nrf2 in young grass carp (Ctenopharyngodon idella)".FISH & SHELLFISH IMMUNOLOGY 40.1(2014):275-287.
Files in This Item: Download All
File Name/Size DocType Version Access License
Dietary tryptophan m(1384KB) 开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wen, Hailang]'s Articles
[Feng, Lin]'s Articles
[Jiang, Weidan]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wen, Hailang]'s Articles
[Feng, Lin]'s Articles
[Jiang, Weidan]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wen, Hailang]'s Articles
[Feng, Lin]'s Articles
[Jiang, Weidan]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Dietary tryptophan modulates intestinal immune response, barrier function, antioxidant status and gene expression of TOR and Nrf2 in young grass carp (Ctenopharyngodon idella).pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.