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题名: The impaired intestinal mucosal immune system by valine deficiency for young grass carp (Ctenopharyngodon idella) is associated with decreasing immune status and regulating tight junction proteins transcript abundance in the intestine
作者: Luo, Jian-Bo1; Feng, Lin1, 2, 3; Jiang, Wei-Dan1, 2, 3; Liu, Yang1, 2, 3; Wu, Pei1, 2, 3; Jiang, Jun1, 2, 3; Kuang, Sheng-Yao4; Tang, Ling4; Zhang, Yong-An5; Zhou, Xiao-Qiu1, 2, 3
关键词: Valine deficiency ; Grass carp ; Immune status ; Tight junction proteins ; Intestine
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2014-09-01
DOI: 10.1016/j.fsi.2014.07.003
卷: 40, 期:1, 页:197-207
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
英文摘要: This study investigated the effects of dietary valine on the growth, intestinal immune response, tight junction proteins transcript abundance and gene expression of immune-related signaling molecules in the intestine of young grass carp (Ctenopharyngodon idella). Six iso-nitrogenous diets containing graded levels of valine (4.3-19.1 g kg(-1) diet) were fed to the fish for 8 weeks. The results showed that percentage weight gain (PWG), feed intake and feed efficiency of fish were the lowest in fish fed the valine-deficient diet (P < 0.05). In addition, valine deficiency decreased lysozyme, acid phosphatase activities and complement 3 content in the intestine (P < 0.05), down-regulated mRNA levels of interleukin 10, transforming growth factor beta 1, I kappa B alpha and target of rapamycin (TOR) (P < 0.05), and up-regulated tumor necrosis factor alpha, interleukin 8 and nuclear factor kappa B P65 (NF-kappa B P65) gene expression (P < 0.05). Additionally, valine deficiency significantly decreased transcript of Occludin. Claudin b, Claudin c, Claudin 3, and ZO-1 (P < 0.05), and improved Claudin 15 expression in the fish intestine (P < 0.05). However, valine did not have a significant effect on expression of Claudin 12 in the intestine of grass carp (P > 0.05). In conclusion, valine deficiency decreased fish growth and intestinal immune status, as well as regulated gene expression of tight junction proteins, NF-kappa B P65, I kappa B alpha and TOR in the fish intestine. Based on the quadratic regression analysis of lysozyme activity or PWG, the dietary valine requirement of young grass carp (268-679 g) were established to be 14.47 g kg(-1) diet (4.82 g 100 g(-1) CP) or 14.00 g kg(-1) diet (4.77 g 100 g(-1) CP), respectively. (c) 2014 Elsevier Ltd. All rights reserved.
关键词[WOS]: TROUT ONCORHYNCHUS-MYKISS ; KAPPA-B ACTIVATION ; SALMON SALMO-SALAR ; INDIAN MAJOR CARP ; FED GRADED-LEVELS ; JUVENILE JIAN ; VAR. JIAN ; AMINO-ACIDS ; NONSPECIFIC IMMUNE ; ULCERATIVE-COLITIS
语种: 英语
WOS记录号: WOS:000341468500023
ISSN号: 1050-4648
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/20383
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Peoples R China
2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov, Chengdu 611130, Peoples R China
3.Sichuan Agr Univ, Minist Educ, Key Lab Anim Dis Resistance Nutr China, Chengdu 611130, Peoples R China
4.Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 610066, Peoples R China
5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
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