IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
The impaired intestinal mucosal immune system by valine deficiency for young grass carp (Ctenopharyngodon idella) is associated with decreasing immune status and regulating tight junction proteins transcript abundance in the intestine
Luo, Jian-Bo1; Feng, Lin1,2,3; Jiang, Wei-Dan1,2,3; Liu, Yang1,2,3; Wu, Pei1,2,3; Jiang, Jun1,2,3; Kuang, Sheng-Yao4; Tang, Ling4; Zhang, Yong-An5; Zhou, Xiao-Qiu1,2,3
2014-09-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume40Issue:1Pages:197-207
AbstractThis study investigated the effects of dietary valine on the growth, intestinal immune response, tight junction proteins transcript abundance and gene expression of immune-related signaling molecules in the intestine of young grass carp (Ctenopharyngodon idella). Six iso-nitrogenous diets containing graded levels of valine (4.3-19.1 g kg(-1) diet) were fed to the fish for 8 weeks. The results showed that percentage weight gain (PWG), feed intake and feed efficiency of fish were the lowest in fish fed the valine-deficient diet (P < 0.05). In addition, valine deficiency decreased lysozyme, acid phosphatase activities and complement 3 content in the intestine (P < 0.05), down-regulated mRNA levels of interleukin 10, transforming growth factor beta 1, I kappa B alpha and target of rapamycin (TOR) (P < 0.05), and up-regulated tumor necrosis factor alpha, interleukin 8 and nuclear factor kappa B P65 (NF-kappa B P65) gene expression (P < 0.05). Additionally, valine deficiency significantly decreased transcript of Occludin. Claudin b, Claudin c, Claudin 3, and ZO-1 (P < 0.05), and improved Claudin 15 expression in the fish intestine (P < 0.05). However, valine did not have a significant effect on expression of Claudin 12 in the intestine of grass carp (P > 0.05). In conclusion, valine deficiency decreased fish growth and intestinal immune status, as well as regulated gene expression of tight junction proteins, NF-kappa B P65, I kappa B alpha and TOR in the fish intestine. Based on the quadratic regression analysis of lysozyme activity or PWG, the dietary valine requirement of young grass carp (268-679 g) were established to be 14.47 g kg(-1) diet (4.82 g 100 g(-1) CP) or 14.00 g kg(-1) diet (4.77 g 100 g(-1) CP), respectively. (c) 2014 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordValine Deficiency Grass Carp Immune Status Tight Junction Proteins Intestine
DOI10.1016/j.fsi.2014.07.003
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000341468500023
WOS KeywordTROUT ONCORHYNCHUS-MYKISS ; KAPPA-B ACTIVATION ; SALMON SALMO-SALAR ; INDIAN MAJOR CARP ; FED GRADED-LEVELS ; JUVENILE JIAN ; VAR. JIAN ; AMINO-ACIDS ; NONSPECIFIC IMMUNE ; ULCERATIVE-COLITIS
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20383
Collection水生生物分子与细胞生物学研究中心_期刊论文
Affiliation1.Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Peoples R China
2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov, Chengdu 611130, Peoples R China
3.Sichuan Agr Univ, Minist Educ, Key Lab Anim Dis Resistance Nutr China, Chengdu 611130, Peoples R China
4.Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 610066, Peoples R China
5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Luo, Jian-Bo,Feng, Lin,Jiang, Wei-Dan,et al. The impaired intestinal mucosal immune system by valine deficiency for young grass carp (Ctenopharyngodon idella) is associated with decreasing immune status and regulating tight junction proteins transcript abundance in the intestine[J]. FISH & SHELLFISH IMMUNOLOGY,2014,40(1):197-207.
APA Luo, Jian-Bo.,Feng, Lin.,Jiang, Wei-Dan.,Liu, Yang.,Wu, Pei.,...&Zhou, Xiao-Qiu.(2014).The impaired intestinal mucosal immune system by valine deficiency for young grass carp (Ctenopharyngodon idella) is associated with decreasing immune status and regulating tight junction proteins transcript abundance in the intestine.FISH & SHELLFISH IMMUNOLOGY,40(1),197-207.
MLA Luo, Jian-Bo,et al."The impaired intestinal mucosal immune system by valine deficiency for young grass carp (Ctenopharyngodon idella) is associated with decreasing immune status and regulating tight junction proteins transcript abundance in the intestine".FISH & SHELLFISH IMMUNOLOGY 40.1(2014):197-207.
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