IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Missing Genes, Multiple ORFs, and C-to-U Type RNA Editing in Acrasis kona (Heterolobosea, Excavata) Mitochondrial DNA
Fu, Cheng-Jie1; Sheikh, Sanea1; Miao, Wei2; Andersson, Siv G. E.3; Baldauf, Sandra L.1
2014-09-01
Source PublicationGENOME BIOLOGY AND EVOLUTION
ISSN1759-6653
Volume6Issue:9Pages:2240-2257
AbstractDiscoba (Excavata) is an ancient group of eukaryotes with great morphological and ecological diversity. Unlike the other major divisions of Discoba (Jakobida and Euglenozoa), little is known about the mitochondrial DNAs(mtDNAs) of Heterolobosea. We have assembled a complete mtDNA genome from the aggregating heterolobosean amoeba, Acrasis kona, which consists of a single circular highly AT-rich (83.3%) molecule of 51.5 kb. Unexpectedly, A. kona mtDNA is missing roughly 40% of the protein-coding genes and nearly half of the transfer RNAs found in the only other sequenced heterolobosean mtDNAs, those of Naegleria spp. Instead, over a quarter of A. kona mtDNA consists of novel open reading frames. Eleven of the 16 protein-coding genes missing from A. kona mtDNA were identified in its nuclear DNA and polyA RNA, and phylogenetic analyses indicate that at least 10 of these 11 putative nuclear-encoded mitochondrial (NcMt) proteins arose by direct transfer from the mitochondrion. Acrasis kona mtDNA also employs C-to-U type RNA editing, and 12 homologs of DYW-type pentatricopeptide repeat (PPR) proteins implicated in plant organellar RNA editing are found in A. kona nuclear DNA. A mapping of mitochondrial gene content onto a consensus phylogeny reveals a sporadic pattern of relative stasis and rampant gene loss in Discoba. Rampant loss occurred independently in the unique common lineage leading to Heterolobosea + Tsukubamonadida and later in the unique lineage leading to Acrasis. Meanwhile, mtDNA gene content appears to be remarkably stable in the Acrasis sister lineage leading to Naegleria and in their distant relatives Jakobida.
SubtypeArticle
KeywordDiscoba Endosymbiotic Gene Transfer Horizontal Gene Transfer Codon Usage Bias Plant-type Rna Editing Split Ribosomal Protein Gene
DOI10.1093/gbe/evu180
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaEvolutionary Biology ; Genetics & Heredity
WOS SubjectEvolutionary Biology ; Genetics & Heredity
WOS IDWOS:000343249300007
WOS KeywordPENTATRICOPEPTIDE REPEAT PROTEINS ; CODON USAGE BIAS ; PHYLOGENETIC RECONSTRUCTION ; ACANTHAMOEBA-CASTELLANII ; REVISED CLASSIFICATION ; TARGETING SEQUENCES ; NAEGLERIA-GRUBERI ; GENOME ; EVOLUTION ; ORIGIN
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20382
Collection水生生物分子与细胞生物学研究中心_期刊论文
Affiliation1.Uppsala Univ, Program Systemat Biol, Dept Organismal Biol, Evolutionary Biol Ctr, Uppsala, Sweden
2.Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan, Peoples R China
3.Uppsala Univ, Dept Mol Evolut Cell & Mol Biol, Sci Life Lab, Biomed Ctr, Uppsala, Sweden
Recommended Citation
GB/T 7714
Fu, Cheng-Jie,Sheikh, Sanea,Miao, Wei,et al. Missing Genes, Multiple ORFs, and C-to-U Type RNA Editing in Acrasis kona (Heterolobosea, Excavata) Mitochondrial DNA[J]. GENOME BIOLOGY AND EVOLUTION,2014,6(9):2240-2257.
APA Fu, Cheng-Jie,Sheikh, Sanea,Miao, Wei,Andersson, Siv G. E.,&Baldauf, Sandra L..(2014).Missing Genes, Multiple ORFs, and C-to-U Type RNA Editing in Acrasis kona (Heterolobosea, Excavata) Mitochondrial DNA.GENOME BIOLOGY AND EVOLUTION,6(9),2240-2257.
MLA Fu, Cheng-Jie,et al."Missing Genes, Multiple ORFs, and C-to-U Type RNA Editing in Acrasis kona (Heterolobosea, Excavata) Mitochondrial DNA".GENOME BIOLOGY AND EVOLUTION 6.9(2014):2240-2257.
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