Functional Divergence of the miRNA Transcriptome at the Onset of Drosophila Metamorphosis
Yeh, Shu-Dan1; von Grotthuss, Marcin1; Gandasetiawan, Kania A.1; Jayasekera, Suvini1; Xia, Xiao-Qin2; Chan, Carolus1; Jayaswal, Vivek3; Ranz, Jose M.1
2014-10-01
Source PublicationMOLECULAR BIOLOGY AND EVOLUTION
ISSN0737-4038
Volume31Issue:10Pages:2557-2572
AbstractMicroRNAs (miRNAs) are endogenous RNA molecules that regulate gene expression posttranscriptionally. To date, the emergence ofmiRNAs and their patterns of sequence evolution have been analyzed in great detail. However, the extent to which miRNA expression levels have evolved over time, the role different evolutionary forces play in shaping these changes, and whether this variation in miRNA expression can reveal the interplay between miRNAs and mRNAs remain poorly understood. This is especially true for miRNA expressed during key developmental transitions. Here, we assayed miRNA expression levels immediately before (>= 18BPF [18 h before puparium formation]) and after (PF) the increase in the hormone ecdysone responsible for triggering metamorphosis. We did so in four strains of Drosophila melanogaster and two closely related species. In contrast to their sequence conservation, approximately 25% of miRNAs analyzed showed significant within-species variation in male expression levels at >= 18BPF and/or PF. Additionally, approximately 33% showed modifications in their pattern of expression bias between developmental timepoints. A separate analysis of the >= 18BPF and PF stages revealed that changes in miRNA abundance accumulate linearly over evolutionary time at PF but not at >= 18BPF. Importantly, >= 18BPF-enriched miRNAs showed the greatest variation in expression levels both within and between species, so are the less likely to evolve under stabilizing selection. Functional attributes, such as expression ubiquity, appeared more tightly associated with lower levels of miRNA expression polymorphism at PF than at >= 18BPF. Furthermore, >= 18BPF- and PF-enriched miRNAs showed opposite patterns of covariation in expression with mRNAs, which denoted the type of regulatory relationship between miRNAs and mRNAs. Collectively, our results show contrasting patterns of functional divergence associated withmiRNA expression levels during Drosophila ontogeny.
SubtypeArticle
KeywordDrosophila Evolution Of Expression prOfiles Mirnas Metamorphosis Mirna-mrna Associations
DOI10.1093/molbev/msu195
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Evolutionary Biology ; Genetics & Heredity
WOS SubjectBiochemistry & Molecular Biology ; Evolutionary Biology ; Genetics & Heredity
WOS KeywordGENE-EXPRESSION ; CODING REGIONS ; POSTTRANSCRIPTIONAL REGULATION ; CELL DIFFERENTIATION ; MICRORNA EXPRESSION ; HORMONAL-REGULATION ; IMMUNE-RESPONSE ; EVOLUTION ; MELANOGASTER ; MECHANISMS
WOS IDWOS:000343402200002
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20380
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Affiliation1.Univ Calif Irvine, Dept Ecol & Evolutionary Biol, Irvine, CA 92717 USA
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
3.Univ Sydney, Sch Math & Stat, Sydney, NSW 2006, Australia
Recommended Citation
GB/T 7714
Yeh, Shu-Dan,von Grotthuss, Marcin,Gandasetiawan, Kania A.,et al. Functional Divergence of the miRNA Transcriptome at the Onset of Drosophila Metamorphosis[J]. MOLECULAR BIOLOGY AND EVOLUTION,2014,31(10):2557-2572.
APA Yeh, Shu-Dan.,von Grotthuss, Marcin.,Gandasetiawan, Kania A..,Jayasekera, Suvini.,Xia, Xiao-Qin.,...&Ranz, Jose M..(2014).Functional Divergence of the miRNA Transcriptome at the Onset of Drosophila Metamorphosis.MOLECULAR BIOLOGY AND EVOLUTION,31(10),2557-2572.
MLA Yeh, Shu-Dan,et al."Functional Divergence of the miRNA Transcriptome at the Onset of Drosophila Metamorphosis".MOLECULAR BIOLOGY AND EVOLUTION 31.10(2014):2557-2572.
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