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题名: Functional Divergence of the miRNA Transcriptome at the Onset of Drosophila Metamorphosis
作者: Yeh, Shu-Dan1; von Grotthuss, Marcin1; Gandasetiawan, Kania A.1; Jayasekera, Suvini1; Xia, Xiao-Qin2; Chan, Carolus1; Jayaswal, Vivek3; Ranz, Jose M.1
关键词: Drosophila ; evolution of expression profiles ; miRNAs ; metamorphosis ; miRNA-mRNA associations
刊名: MOLECULAR BIOLOGY AND EVOLUTION
发表日期: 2014-10-01
DOI: 10.1093/molbev/msu195
卷: 31, 期:10, 页:2557-2572
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ; Evolutionary Biology ; Genetics & Heredity
研究领域[WOS]: Biochemistry & Molecular Biology ; Evolutionary Biology ; Genetics & Heredity
英文摘要: MicroRNAs (miRNAs) are endogenous RNA molecules that regulate gene expression posttranscriptionally. To date, the emergence ofmiRNAs and their patterns of sequence evolution have been analyzed in great detail. However, the extent to which miRNA expression levels have evolved over time, the role different evolutionary forces play in shaping these changes, and whether this variation in miRNA expression can reveal the interplay between miRNAs and mRNAs remain poorly understood. This is especially true for miRNA expressed during key developmental transitions. Here, we assayed miRNA expression levels immediately before (>= 18BPF [18 h before puparium formation]) and after (PF) the increase in the hormone ecdysone responsible for triggering metamorphosis. We did so in four strains of Drosophila melanogaster and two closely related species. In contrast to their sequence conservation, approximately 25% of miRNAs analyzed showed significant within-species variation in male expression levels at >= 18BPF and/or PF. Additionally, approximately 33% showed modifications in their pattern of expression bias between developmental timepoints. A separate analysis of the >= 18BPF and PF stages revealed that changes in miRNA abundance accumulate linearly over evolutionary time at PF but not at >= 18BPF. Importantly, >= 18BPF-enriched miRNAs showed the greatest variation in expression levels both within and between species, so are the less likely to evolve under stabilizing selection. Functional attributes, such as expression ubiquity, appeared more tightly associated with lower levels of miRNA expression polymorphism at PF than at >= 18BPF. Furthermore, >= 18BPF- and PF-enriched miRNAs showed opposite patterns of covariation in expression with mRNAs, which denoted the type of regulatory relationship between miRNAs and mRNAs. Collectively, our results show contrasting patterns of functional divergence associated withmiRNA expression levels during Drosophila ontogeny.
关键词[WOS]: GENE-EXPRESSION ; CODING REGIONS ; POSTTRANSCRIPTIONAL REGULATION ; CELL DIFFERENTIATION ; MICRORNA EXPRESSION ; HORMONAL-REGULATION ; IMMUNE-RESPONSE ; EVOLUTION ; MELANOGASTER ; MECHANISMS
语种: 英语
WOS记录号: WOS:000343402200002
ISSN号: 0737-4038
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/20380
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Univ Calif Irvine, Dept Ecol & Evolutionary Biol, Irvine, CA 92717 USA
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
3.Univ Sydney, Sch Math & Stat, Sydney, NSW 2006, Australia

Recommended Citation:
Yeh, Shu-Dan; von Grotthuss, Marcin; Gandasetiawan, Kania A.; Jayasekera, Suvini; Xia, Xiao-Qin; Chan, Carolus; Jayaswal, Vivek; Ranz, Jose M..Functional Divergence of the miRNA Transcriptome at the Onset of Drosophila Metamorphosis,MOLECULAR BIOLOGY AND EVOLUTION,2014,():
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