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Analysis of phosphoproteome in rice pistil
Wang, Kun1; Zhao, Yong2; Li, Ming1; Gao, Feng3; Yang, Ming-kun4; Wang, Xin1; Li, Shaoqing3; Yang, Pingfang1
2014-10-01
Source PublicationPROTEOMICS
ISSN1615-9853
Volume14Issue:20Pages:2319-2334
AbstractAs the female reproductive part of a flower, the pistil consists of the ovary, style, and stigma, and is a critical organ for the process from pollen recognition to fertilization and seed formation. Previous studies on pollen-pistil interaction mainly focused on gene expression changes with comparative transcriptomics or proteomics method. However, studies on protein PTMs are still lacking. Here we report a phosphoproteomic study on mature pistil of rice. Using IMAC enrichment, hydrophilic interaction chromatography fraction and high-accuracy MS instrument (TripleTOF 5600), 2347 of high-confidence (Ascore >= 19, p <= 0.01), phosphorylation sites corresponding to 1588 phosphoproteins were identified. Among them, 1369 phosphorylation sites within 654 phosphoproteins were newly identified; 41 serine phosphorylation motifs, which belong to three groups: proline-directed, basophilic, and acidic motifs were identified after analysis by motif-X. Two hundred and one genes whose phosphopeptides were identified here showed tissue-specific expression in pistil based on information mining of previous microarray data. All MS data have been deposited in the ProteomeXchange with identifier PXD000923 (http://proteomecentral.proteomexchange.org/dataset/PXD000923). This study will help us to understand pistil development and pollination on the posttranslational level.
SubtypeArticle
KeywordImac Oryza Sativa L. Phosphoproteome Pistil Plant Proteomics Pollen-pistil Interaction
DOI10.1002/pmic.201400004
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemical Research Methods ; Biochemistry & Molecular Biology
WOS IDWOS:000343833700010
WOS KeywordSELF-INCOMPATIBILITY RESPONSE ; LOCUS RECEPTOR KINASE ; POLLEN-TUBE GROWTH ; PROTEIN-PHOSPHORYLATION ANALYSIS ; GENE-EXPRESSION ; S-LOCUS ; ARABIDOPSIS POLLEN ; ORYZA-SATIVA ; PROTEOMIC ANALYSIS ; PROFILING REVEALS
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20378
Collection水生生物分子与细胞生物学研究中心_期刊论文
Affiliation1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Peoples R China
2.Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China
3.Wuhan Univ, Coll Life Sci, State Key Lab Hybrid Rice, Wuhan 430072, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Peoples R China
Recommended Citation
GB/T 7714
Wang, Kun,Zhao, Yong,Li, Ming,et al. Analysis of phosphoproteome in rice pistil[J]. PROTEOMICS,2014,14(20):2319-2334.
APA Wang, Kun.,Zhao, Yong.,Li, Ming.,Gao, Feng.,Yang, Ming-kun.,...&Yang, Pingfang.(2014).Analysis of phosphoproteome in rice pistil.PROTEOMICS,14(20),2319-2334.
MLA Wang, Kun,et al."Analysis of phosphoproteome in rice pistil".PROTEOMICS 14.20(2014):2319-2334.
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