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题名: Analysis of phosphoproteome in rice pistil
作者: Wang, Kun1; Zhao, Yong2; Li, Ming1; Gao, Feng3; Yang, Ming-kun4; Wang, Xin1; Li, Shaoqing3; Yang, Pingfang1
关键词: IMAC ; Oryza sativa L. ; Phosphoproteome ; Pistil ; Plant proteomics ; Pollen-pistil interaction
刊名: PROTEOMICS
发表日期: 2014-10-01
DOI: 10.1002/pmic.201400004
卷: 14, 期:20, 页:2319-2334
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods ; Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: As the female reproductive part of a flower, the pistil consists of the ovary, style, and stigma, and is a critical organ for the process from pollen recognition to fertilization and seed formation. Previous studies on pollen-pistil interaction mainly focused on gene expression changes with comparative transcriptomics or proteomics method. However, studies on protein PTMs are still lacking. Here we report a phosphoproteomic study on mature pistil of rice. Using IMAC enrichment, hydrophilic interaction chromatography fraction and high-accuracy MS instrument (TripleTOF 5600), 2347 of high-confidence (Ascore >= 19, p <= 0.01), phosphorylation sites corresponding to 1588 phosphoproteins were identified. Among them, 1369 phosphorylation sites within 654 phosphoproteins were newly identified; 41 serine phosphorylation motifs, which belong to three groups: proline-directed, basophilic, and acidic motifs were identified after analysis by motif-X. Two hundred and one genes whose phosphopeptides were identified here showed tissue-specific expression in pistil based on information mining of previous microarray data. All MS data have been deposited in the ProteomeXchange with identifier PXD000923 (http://proteomecentral.proteomexchange.org/dataset/PXD000923). This study will help us to understand pistil development and pollination on the posttranslational level.
关键词[WOS]: SELF-INCOMPATIBILITY RESPONSE ; LOCUS RECEPTOR KINASE ; POLLEN-TUBE GROWTH ; PROTEIN-PHOSPHORYLATION ANALYSIS ; GENE-EXPRESSION ; S-LOCUS ; ARABIDOPSIS POLLEN ; ORYZA-SATIVA ; PROTEOMIC ANALYSIS ; PROFILING REVEALS
语种: 英语
WOS记录号: WOS:000343833700010
ISSN号: 1615-9853
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/20378
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Peoples R China
2.Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China
3.Wuhan Univ, Coll Life Sci, State Key Lab Hybrid Rice, Wuhan 430072, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Peoples R China

Recommended Citation:
Wang, Kun; Zhao, Yong; Li, Ming; Gao, Feng; Yang, Ming-kun; Wang, Xin; Li, Shaoqing; Yang, Pingfang.Analysis of phosphoproteome in rice pistil,PROTEOMICS,2014,():
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