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题名: Differential growth performance, intestinal antioxidant status and relative expression of Nrf2 and its target genes in young grass carp (Ctenopharyngodon idella) fed with graded levels of leucine
作者: Deng, Yu-Ping1; Jiang, Wei-Dan1, 2, 3; Liu, Yang1, 2, 3; Jiang, Jun1, 2, 3; Kuang, Sheng-Yao4; Tang, Ling4; Wu, Pei1, 2, 3; Zhang, Yong-An5; Feng, Lin1, 2, 3; Zhou, Xiao-Qiu1, 2, 3
关键词: Grass carp (Ctenopharyngodon idella) ; Leucine requirement ; Antioxidant enzyme ; Gene expression ; Nrf2 ; Kelch-like ECH associated protein 1
刊名: AQUACULTURE
发表日期: 2014-10-20
DOI: 10.1016/j.aquaculture.2014.07.026
卷: 434, 页:66-73
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Fisheries ; Marine & Freshwater Biology
研究领域[WOS]: Fisheries ; Marine & Freshwater Biology
英文摘要: The aim of the study was to evaluate the effects of dietary leucine on growth performance, intestinal antioxidant status and relative expression of Nrf2 and its target genes in young grass carp (Ctenophazyngodon idella). A total of 540 young grass carp (295.85 +/- 2.07 g) were fed six isonitrogenous diets with graded levels of leucine (7.1, 8.9, 11.0, 13.3, 15.2 and 17.1 g kg(-1) diet) for 8 weeks. Results indicated that percent weight gain (PWG), feed intake, feed efficiency, intestine length, intestine weight and intestine protein content were significantly improved by dietary leucine. Similarly, optimum leucine supplementation significantly increased intestinal glutathione content, copper/zinc superoxide dismutase (CuZnSOD) and glutathione peroxidase (GPx) activities as compared with the control group. The changes in intestinal CuZnSOD, GPx and nuclear factor erythroid 2-related factor 2 (Nrf2) mRNA levels induced by leucine were similar to those noted in the enzyme activities. Plasma ammonia concentration (PAC), intestinal malondialdehyde (MDA) and protein carbonyl contents, as well as the mRNA level of Kelch-like ECH-associated protein 1 (Keap1) exhibited an opposite tendency. Interestingly, dietary leucine level higher than 13.3 or 15.2 g kg(-1) diet has adverse effect on intestinal antioxidant status. Collectively, our results indicated that optimum leucine supplementation improved fish growth and antioxidant capacity, which may be partly related to the Nrf2 signalling pathway. Based on PWG, PAC and intestinal MDA, the optimum dietary leucine requirements of young grass carp (296-690 g) were estimated to be 13.0, 12.9 and 13.2 g kg(-1) diet, corresponding to 42.4, 42.0 and 43.0 g kg(-1) of dietary protein, respectively. (C) 2014 Elsevier B.V. All rights reserved.
关键词[WOS]: TROUT ONCORHYNCHUS-MYKISS ; INDUCED OXIDATIVE DAMAGE ; FINGERLING CHANNEL CATFISH ; SALMON SALMO-SALAR ; INDIAN MAJOR CARP ; DIETARY FISH-OIL ; RAINBOW-TROUT ; LITOPENAEUS-VANNAMEI ; FORMULATED DIETS ; BODY-COMPOSITION
语种: 英语
WOS记录号: WOS:000345058700010
ISSN号: 0044-8486
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/20377
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China
2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov, Sichuan, Cheng Du, Peoples R China
3.Sichuan Agr Univ, Key Lab Anim Dis Resistance Nutr, China Minist Educ, Sichuan, Cheng Du, Peoples R China
4.Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 611130, Peoples R China
5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China

Recommended Citation:
Deng, Yu-Ping; Jiang, Wei-Dan; Liu, Yang; Jiang, Jun; Kuang, Sheng-Yao; Tang, Ling; Wu, Pei; Zhang, Yong-An; Feng, Lin; Zhou, Xiao-Qiu.Differential growth performance, intestinal antioxidant status and relative expression of Nrf2 and its target genes in young grass carp (Ctenopharyngodon idella) fed with graded levels of leucine,AQUACULTURE,2014,():
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