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Differential growth performance, intestinal antioxidant status and relative expression of Nrf2 and its target genes in young grass carp (Ctenopharyngodon idella) fed with graded levels of leucine
Deng, Yu-Ping1; Jiang, Wei-Dan1,2,3; Liu, Yang1,2,3; Jiang, Jun1,2,3; Kuang, Sheng-Yao4; Tang, Ling4; Wu, Pei1,2,3; Zhang, Yong-An5; Feng, Lin1,2,3; Zhou, Xiao-Qiu1,2,3
2014-10-20
Source PublicationAQUACULTURE
ISSN0044-8486
Volume434Pages:66-73
AbstractThe aim of the study was to evaluate the effects of dietary leucine on growth performance, intestinal antioxidant status and relative expression of Nrf2 and its target genes in young grass carp (Ctenophazyngodon idella). A total of 540 young grass carp (295.85 +/- 2.07 g) were fed six isonitrogenous diets with graded levels of leucine (7.1, 8.9, 11.0, 13.3, 15.2 and 17.1 g kg(-1) diet) for 8 weeks. Results indicated that percent weight gain (PWG), feed intake, feed efficiency, intestine length, intestine weight and intestine protein content were significantly improved by dietary leucine. Similarly, optimum leucine supplementation significantly increased intestinal glutathione content, copper/zinc superoxide dismutase (CuZnSOD) and glutathione peroxidase (GPx) activities as compared with the control group. The changes in intestinal CuZnSOD, GPx and nuclear factor erythroid 2-related factor 2 (Nrf2) mRNA levels induced by leucine were similar to those noted in the enzyme activities. Plasma ammonia concentration (PAC), intestinal malondialdehyde (MDA) and protein carbonyl contents, as well as the mRNA level of Kelch-like ECH-associated protein 1 (Keap1) exhibited an opposite tendency. Interestingly, dietary leucine level higher than 13.3 or 15.2 g kg(-1) diet has adverse effect on intestinal antioxidant status. Collectively, our results indicated that optimum leucine supplementation improved fish growth and antioxidant capacity, which may be partly related to the Nrf2 signalling pathway. Based on PWG, PAC and intestinal MDA, the optimum dietary leucine requirements of young grass carp (296-690 g) were estimated to be 13.0, 12.9 and 13.2 g kg(-1) diet, corresponding to 42.4, 42.0 and 43.0 g kg(-1) of dietary protein, respectively. (C) 2014 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordGrass Carp (Ctenopharyngodon Idella) Leucine Requirement Antioxidant Enzyme Gene Expression Nrf2 Kelch-like Ech Associated Protein 1
DOI10.1016/j.aquaculture.2014.07.026
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000345058700010
WOS KeywordTROUT ONCORHYNCHUS-MYKISS ; INDUCED OXIDATIVE DAMAGE ; FINGERLING CHANNEL CATFISH ; SALMON SALMO-SALAR ; INDIAN MAJOR CARP ; DIETARY FISH-OIL ; RAINBOW-TROUT ; LITOPENAEUS-VANNAMEI ; FORMULATED DIETS ; BODY-COMPOSITION
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Cited Times:54[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20377
Collection水生生物分子与细胞生物学研究中心_期刊论文
Affiliation1.Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China
2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov, Sichuan, Cheng Du, Peoples R China
3.Sichuan Agr Univ, Key Lab Anim Dis Resistance Nutr, China Minist Educ, Sichuan, Cheng Du, Peoples R China
4.Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 611130, Peoples R China
5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Deng, Yu-Ping,Jiang, Wei-Dan,Liu, Yang,et al. Differential growth performance, intestinal antioxidant status and relative expression of Nrf2 and its target genes in young grass carp (Ctenopharyngodon idella) fed with graded levels of leucine[J]. AQUACULTURE,2014,434:66-73.
APA Deng, Yu-Ping.,Jiang, Wei-Dan.,Liu, Yang.,Jiang, Jun.,Kuang, Sheng-Yao.,...&Zhou, Xiao-Qiu.(2014).Differential growth performance, intestinal antioxidant status and relative expression of Nrf2 and its target genes in young grass carp (Ctenopharyngodon idella) fed with graded levels of leucine.AQUACULTURE,434,66-73.
MLA Deng, Yu-Ping,et al."Differential growth performance, intestinal antioxidant status and relative expression of Nrf2 and its target genes in young grass carp (Ctenopharyngodon idella) fed with graded levels of leucine".AQUACULTURE 434(2014):66-73.
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