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Investigation of effect of 17 alpha-ethinylestradiol on vigilin expression using an isolated recombinant antibody
Zhong, Liqiao1; Yuan, Li1; Rao, Yu1; Li, Zhouquan1,2; Gu, Qilin1,2; Long, Yong1; Zhang, Xiaohua1; Cui, Zongbin1; Xu, Ying1; Dai, Heping1
2014-11-01
Source PublicationAQUATIC TOXICOLOGY
ISSN0166-445X
Volume156Pages:1-9
AbstractVigilin, a highly conserved protein from yeast to mammals, is a multifunctional protein in eukaryotic organisms. One biological function of vigilin is to stabilize the expression level of vitellogenin (VTG). This study aimed to develop vigilin as a new estrogen-inducible biomarker that correlates with the widely applied estrogen-inducible biomarker VTG and expand the ability to detect it in various species. Here, a recombinant monoclonal antibody with high specificity against the conserved C-terminal region of vigilin from zebrafish (Danio rerio) was successfully isolated from a phage display antibody library and found to recognize vigilin proteins from multiple vertebrate species. The effect of 17 alpha-ethinylestradiol (EE2) on vigilin expression in the liver of zebrafish and juvenile crucian carp (Carassius auratus) was investigated. Although vigilin mRNA was expressed in all tissues analyzed from male zebrafish, vigilin protein was detected exclusively in the testis of male zebrafish, as well as the liver of female zebrafish and juvenile crucian carp at a lower level without exposure to EE2. Significant induction of vigilin mRNA by exposure to EE2 was observed in the liver and testis of male zebrafish, even at a low dose of 6.25 ng/L (21.09 pmol/L). In Hela cells, the expression of vigilin coincided with high protein synthesis activity but not dose-dependently by EE2 exposure. Therefore, the recombinant antibody may be used as a detection tool to screen for mammalian cell lines or organs with estrogen-inducible expression of vigilin. (C) 2014 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordVigilin Phage Display Single-chain Variable Fragment (Scfv) 17 Alpha-ethinylestradiol (Ee2) Zebrafish Crucian Carp
DOI10.1016/j.aquatox.2014.07.016
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaMarine & Freshwater Biology ; Toxicology
WOS SubjectMarine & Freshwater Biology ; Toxicology
WOS IDWOS:000345190000001
WOS KeywordVITELLOGENIN MESSENGER-RNA ; KH-DOMAIN PROTEIN ; LIPOPROTEIN-BINDING-PROTEIN ; SACCHAROMYCES-CEREVISIAE ; ENDOPLASMIC-RETICULUM ; CHICKEN VIGILIN ; SCP160P ; YEAST ; SEQUENCE ; CELLS
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20376
Collection水生生物分子与细胞生物学研究中心_期刊论文
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Fresh Water Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Zhong, Liqiao,Yuan, Li,Rao, Yu,et al. Investigation of effect of 17 alpha-ethinylestradiol on vigilin expression using an isolated recombinant antibody[J]. AQUATIC TOXICOLOGY,2014,156:1-9.
APA Zhong, Liqiao.,Yuan, Li.,Rao, Yu.,Li, Zhouquan.,Gu, Qilin.,...&Dai, Heping.(2014).Investigation of effect of 17 alpha-ethinylestradiol on vigilin expression using an isolated recombinant antibody.AQUATIC TOXICOLOGY,156,1-9.
MLA Zhong, Liqiao,et al."Investigation of effect of 17 alpha-ethinylestradiol on vigilin expression using an isolated recombinant antibody".AQUATIC TOXICOLOGY 156(2014):1-9.
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