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题名: Acetylome Analysis Reveals Diverse Functions of Lysine Acetylation in Mycobacterium tuberculosis
作者: Liu, Fengying1; Yang, Mingkun2; Wang, Xude1; Yang, Shanshan1; Gu, Jing1; Zhou, Jie3; Zhang, Xian-En4; Deng, Jiaoyu1; Ge, Feng2
关键词: TANDEM MASS-SPECTROMETRY ; BACTERIUM BACILLUS-SUBTILIS ; M-BOVIS BCG ; ESCHERICHIA-COLI ; COA SYNTHETASE ; SACCHAROMYCES-CEREVISIAE ; PHOSPHOPROTEOME ANALYSIS ; PROTEIN IDENTIFICATION ; REVERSIBLE ACETYLATION ; PLASMODIUM-FALCIPARUM
刊名: MOLECULAR & CELLULAR PROTEOMICS
发表日期: 2014-12-01
DOI: 10.1074/mcp.M114.041962
卷: 13, 期:12, 页:3352-3366
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: The lysine acetylation of proteins is a reversible post-translational modification that plays a critical regulatory role in both eukaryotes and prokaryotes. Mycobacterium tuberculosis is a facultative intracellular pathogen and the causative agent of tuberculosis. Increasing evidence shows that lysine acetylation may play an important role in the pathogenesis of M. tuberculosis. However, only a few acetylated proteins of M. tuberculosis are known, presenting a major obstacle to understanding the functional roles of reversible lysine acetylation in this pathogen. We performed a global acetylome analysis of M. tuberculosis H37Ra by combining protein/peptide prefractionation, antibody enrichment, and LC-MS/MS. In total, we identified 226 acetylation sites in 137 proteins of M. tuberculosis H37Ra. The identified acetylated proteins were functionally categorized into an interaction map and shown to be involved in various biological processes. Consistent with previous reports, a large proportion of the acetylation sites were present on proteins involved in glycolysis/gluconeogenesis, the citrate cycle, and fatty acid metabolism. A NAD(+)-dependent deacetylase (MRA_1161) deletion mutant of M. tuberculosis H37Ra was constructed and its characterization showed a different colony morphology, reduced biofilm formation, and increased tolerance of heat stress. Interestingly, lysine acetylation was found, for the first time, to block the immunogenicity of a peptide derived from a known immunogen, HspX, suggesting that lysine acetylation plays a regulatory role in immunogenicity. Our data provide the first global survey of lysine acetylation in M. tuberculosis. The dataset should be an important resource for the functional analysis of lysine acetylation in M. tuberculosis and facilitate the clarification of the entire metabolic networks of this life-threatening pathogen.
关键词[WOS]: TANDEM MASS-SPECTROMETRY ; BACTERIUM BACILLUS-SUBTILIS ; M-BOVIS BCG ; ESCHERICHIA-COLI ; COA SYNTHETASE ; SACCHAROMYCES-CEREVISIAE ; PHOSPHOPROTEOME ANALYSIS ; PROTEIN IDENTIFICATION ; REVERSIBLE ACETYLATION ; PLASMODIUM-FALCIPARUM
语种: 英语
WOS记录号: WOS:000345626400011
ISSN号: 1535-9476
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/20372
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
3.Foshan Fourth Peoples Hosp, Foshan, Peoples R China
4.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China

Recommended Citation:
Liu, Fengying; Yang, Mingkun; Wang, Xude; Yang, Shanshan; Gu, Jing; Zhou, Jie; Zhang, Xian-En; Deng, Jiaoyu; Ge, Feng.Acetylome Analysis Reveals Diverse Functions of Lysine Acetylation in Mycobacterium tuberculosis,MOLECULAR & CELLULAR PROTEOMICS,2014,():
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