MOLECULAR CLONING, EXPRESSION, AND IN SILICO ANALYSIS OF A LONG TYPE PEPTIDOGLYCAN RECOGNITION PROTEIN FROM HALF-SMOOTH TONGUE SOLE, CYNOGLOSSUS SEMILAEVIS (ACTINOPTERYGII: PLEURONECTIFORMES: CYNOGLOSSIDAE)
Science & Technology
; Life Sciences & Biomedicine
Background. Half-smooth tongue sole, Cynoglossus semilaevis Gunther, 1873, a marine teleost, is an important aquaculture species of great economic value. In recent years, its farm production increase coincided, however, with the number of reported cases of bacterial diseases. Further understanding of its immune response to bacterial pathogens can provide more information on pathogenesis and how to prevent disease using immune-related strategy. Peptidoglycan (PGN) recognition proteins (PGRPs) play important roles in the innate immunity against bacterial infection. In the presently reported study, a long type PGRP in half-smooth tongue sole (csPGRP-L) was cloned, and its sequence features, PGN binding ability, and mRNA expressions in different tissues after bacterial infection were also analyzed.