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题名: Identification of apoptosis-related genes and transcription variations in response to microcystin-LR in zebrafish liver
作者: Wei, Lili1, 2; Hoole, Dave3; Sun, Baojian2
关键词: Microcystin-LR ; apoptosis ; microarray ; real-time PCR ; zebrafish
刊名: TOXICOLOGY AND INDUSTRIAL HEALTH
发表日期: 2014-10-01
DOI: 10.1177/0748233712462443
卷: 30, 期:9, 页:777-784
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Public, Environmental & Occupational Health ; Toxicology
研究领域[WOS]: Public, Environmental & Occupational Health ; Toxicology
英文摘要: There is growing evidence that the effects of microcystin-LR (MC-LR) are closely related to apoptosis. This study utilized microarray to identify the apoptosis-related genes induced by MC-LR in zebrafish liver. The messenger RNA abundance of some apoptosis-related genes was found to be increased, including five tumor necrosis factor (TNF)-related members (apoptosis regulatory protein siva, tumor necrosis factor-alpha (tnfa) TNF (ligand) superfamily member 10 (tnfsf10), TNF-inducible protein 6 (tnfaip6) and TNF receptor associated factor 2 binding protein (traf2bp)), three p53-related genes (tumor protein p53 inducible nuclear protein 1 (tp53inp1), p53-induced protein phosphatase 1 (ppm1d) and a novel apoptosis stimulating protein of p53 (aspp2)), bcl 2 family members (proapoptosis gene bax and antiapoptosis gene mcl 1), caspases (caspase y (caspy) and a PYD and CARD domain-containing protein (pycard)) and the transforming growth factor beta (TGF-beta) induced apoptosis protein 2 (taip2). Real-time polymerase chain reaction was used to study the kinetic transcriptional changes in seven apoptosis-related genes. Elevated transcription of p53, tp53inp1, mcl 1 and taip2 could only be detected at 6 h, increased transcription of the antagonist molecules, bcl 2 and bax could be detected at most time points and the significant change of caspy could be found at 48 h and 72 h after stimulation. Taken together, the results obtained in the present study clearly demonstrate that large amount of apoptosis-related genes are involved in the regulation of MC-LR-induced apoptosis.
关键词[WOS]: CARP CTENOPHARYNGODON-IDELLA ; IN-VIVO ; CYANOBACTERIAL TOXIN ; CELL-DEATH ; OXIDATIVE STRESS ; DANIO-RERIO ; JNK ; P53 ; EXPRESSION ; FISH
语种: 英语
WOS记录号: WOS:000342991800001
ISSN号: 0748-2337
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/20355
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Jiangxi Agr Univ, Coll Anim Sci & Technol, Nanchang, Jiangxi, Peoples R China
2.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Peoples R China
3.Keele Univ, Sch Life Sci, Keele ST5 5BG, Staffs, England
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