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Type-IV Antifreeze Proteins are Essential for Epiboly and Convergence in Gastrulation of Zebrafish Embryos
Xiao, Qing; Xia, Jian-Hong; Zhang, Xiao-Juan; Li, Zhi; Wang, Yang; Zhou, Li; Gui, Jian-Fang
2014
Source PublicationINTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
ISSN1449-2288
Volume10Issue:7Pages:715-732
AbstractMany organisms in extremely cold environments such as the Antarctic Pole have evolved antifreeze molecules to prevent ice formation. There are four types of antifreeze proteins (AFPs). Type-IV antifreeze proteins (AFP4s) are present also in certain temperate and even tropical fish, which has raised a question as to whether these AFP4s have important functions in addition to antifreeze activity. Here we report the identification and functional analyses of AFP4s in cyprinid fish. Two genes, namely afp4a and afp4b coding for AFP4s, were identified in gibel carp (Carassius auratus gibelio) and zebrafish (Danio rerio). In both species, afp4a and afp4b display a head-to-tail tandem arrangement and share a common 4-exonic gene structure. In zebrafish, both afp4a and afp4b were found to express specifically in the yolk syncytial layer (YSL). Interestingly, afp4a expression continues in YSL and digestive system from early embryos to adults, whereas afp4b expression is restricted to embryogenesis. Importantly, we have shown by using afp4a-specific and afp4b-specifc morpholino knockdown and cell lineage tracing approaches that AFP4a participates in epiboly progression by stabilizing yolk cytoplasmic layer microtubules, and AFP4b is primarily related to convergence movement. Therefore, both AFP4 proteins are essential for gastrulation of zebrafish embryos. Our current results provide first evidence that AFP such as AFP4 has important roles in regulating developmental processes besides its well-known function as antifreeze factors.
SubtypeArticle
KeywordAntifreeze Protein Yolk Syncytial Layer Epiboly Convergence Morphogenesis
DOI10.7150/ijbs.9126
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics
WOS SubjectBiochemistry & Molecular Biology ; Biology
WOS IDWOS:000339317100006
WOS KeywordCARASSIUS-AURATUS-GIBELIO ; YOLK SYNCYTIAL LAYER ; EXTENSION MOVEMENTS ; CELL MOVEMENTS ; MYOXOCEPHALUS-OCTODECIMSPINOSIS ; EXPRESSION PATTERN ; LONGHORN SCULPIN ; EMBRYOGENESIS ; FISH ; GENES
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Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20354
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
AffiliationUniv Chinese Acad Sci, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Xiao, Qing,Xia, Jian-Hong,Zhang, Xiao-Juan,et al. Type-IV Antifreeze Proteins are Essential for Epiboly and Convergence in Gastrulation of Zebrafish Embryos[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,2014,10(7):715-732.
APA Xiao, Qing.,Xia, Jian-Hong.,Zhang, Xiao-Juan.,Li, Zhi.,Wang, Yang.,...&Gui, Jian-Fang.(2014).Type-IV Antifreeze Proteins are Essential for Epiboly and Convergence in Gastrulation of Zebrafish Embryos.INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,10(7),715-732.
MLA Xiao, Qing,et al."Type-IV Antifreeze Proteins are Essential for Epiboly and Convergence in Gastrulation of Zebrafish Embryos".INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES 10.7(2014):715-732.
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