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Rana grylio virus (RGV) envelope protein 2L: subcellular localization and essential roles in virus infectivity revealed by conditional lethal mutant
He, Li-Bo; Ke, Fei; Wang, Jun; Gao, Xiao-Chan; Zhang, Qi-Ya
2014-03-01
Source PublicationJOURNAL OF GENERAL VIROLOGY
ISSN0022-1317
Volume95Issue:-Pages:679-690
AbstractRana grylio virus (RGV) is a pathogenic iridovirus that has resulted in high mortality in cultured frog. Here, an envelope protein gene, 2L, was identified from RGV and its possible role in virus infection was investigated. Database searches found that RGV 2L had homologues in all sequenced iridoviruses and is a core gene of iridoviruses. Western blotting detection of purified RGV virions confirmed that 2L protein was associated with virion membrane. Fluorescence localization revealed that 2L protein co-localized with viral factories in RGV infected cells. In co-transfected cells, 2L protein co-localized with two other viral envelope proteins, 22R and 53R. However, 2L protein did not co-localize With the major capsid protein of RGV in co-transfected cells. Meanwhile, fluorescence observation showed that 2L protein co-localized with endoplasmic reticulum, but did not co-localize with mitochondria and Golgi apparatus. Moreover, a conditional lethal mutant virus containing the lac repressor/operator system was constructed to investigate the role of RGV 2L in virus infection. The ability to form plaques and the virus titres were strongly reduced when expression of 2L was repressed. Therefore, the current data showed that 2L protein is essential for virus infection. Our study is the first report, to our knowledge, of co-localization between envelope proteins in iridovirus and provides new insights into the understanding of envelope proteins in iridovirus.
SubtypeArticle
KeywordEndoplasmic-reticulum Family-iridoviridae Proteomic Analysis Fish Cells In-vitro Orf 53r Mu-ns Gene Replication Identification
DOI10.1099/vir.0.058776-0
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Virology
WOS SubjectBiotechnology & Applied Microbiology ; Virology
WOS IDWOS:000338177500016
WOS KeywordENDOPLASMIC-RETICULUM ; FAMILY-IRIDOVIRIDAE ; PROTEOMIC ANALYSIS ; FISH CELLS ; IN-VITRO ; ORF 53R ; MU-NS ; GENE ; REPLICATION ; IDENTIFICATION
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Cited Times:20[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20353
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
AffiliationChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
He, Li-Bo,Ke, Fei,Wang, Jun,et al. Rana grylio virus (RGV) envelope protein 2L: subcellular localization and essential roles in virus infectivity revealed by conditional lethal mutant[J]. JOURNAL OF GENERAL VIROLOGY,2014,95(-):679-690.
APA He, Li-Bo,Ke, Fei,Wang, Jun,Gao, Xiao-Chan,&Zhang, Qi-Ya.(2014).Rana grylio virus (RGV) envelope protein 2L: subcellular localization and essential roles in virus infectivity revealed by conditional lethal mutant.JOURNAL OF GENERAL VIROLOGY,95(-),679-690.
MLA He, Li-Bo,et al."Rana grylio virus (RGV) envelope protein 2L: subcellular localization and essential roles in virus infectivity revealed by conditional lethal mutant".JOURNAL OF GENERAL VIROLOGY 95.-(2014):679-690.
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