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Edwardsiella tarda-Induced Cytotoxicity Depends on Its Type III Secretion System and Flagellin
Xie, Hai-Xia1,2; Lu, Jin-Fang1; Rolhion, Nathalie2; Holden, David W.2; Nie, Pin1; Zhou, Ying1; Yu, Xiu-Jun2
2014-08-01
Source PublicationINFECTION AND IMMUNITY
ISSN0019-9567
Volume82Issue:8Pages:3436-3445
AbstractMany Gram-negative bacteria utilize a type III secretion system (T3SS) to translocate virulence proteins into host cells to cause diseases. In responding to infection, macrophages detect some of the translocated proteins to activate caspase-1-mediated cell death, called pyroptosis, and secretion of proinflammatory cytokines to control the infection. Edwardsiella tarda is a Gram-negative enteric pathogen that causes hemorrhagic septicemia in fish and both gastrointestinal and extraintestinal infections in humans. In this study, we report that the T3SS of E. tarda facilitates its survival and replication in murine bone marrow-derived macrophages, and E. tarda infection triggers pyroptosis of infected macrophages from mice and fish and increased secretion of the cytokine interleukin 1 beta in a T3SS-dependent manner. Deletion of the flagellin gene fliC of E. tarda results in decreased cytotoxicity for infected macrophages and does not attenuate its virulence in a fish model of infection, whereas upregulated expression of FliC in the fliC mutant strain reduces its virulence. We propose that the host controls E. tarda infection partially by detecting FliC translocated by the T3SS, whereas the bacteria downregulate the expression of FliC to evade innate immunity.
SubtypeArticle
KeywordEnterica Serovar Typhimurium Cell-death Salmonella-enterica Gene-expression In-vivo 2-component System Nlrc4 Inflammasome Escherichia-coli Phop-phoq Host
DOI10.1128/IAI.01065-13
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaImmunology ; Infectious Diseases
WOS SubjectImmunology ; Infectious Diseases
WOS IDWOS:000339161400032
WOS KeywordENTERICA SEROVAR TYPHIMURIUM ; CELL-DEATH ; SALMONELLA-ENTERICA ; GENE-EXPRESSION ; IN-VIVO ; 2-COMPONENT SYSTEM ; NLRC4 INFLAMMASOME ; ESCHERICHIA-COLI ; PHOP-PHOQ ; HOST
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Cited Times:20[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20348
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
2.Univ London Imperial Coll Sci Technol & Med, Ctr Mol Bacteriol & Infect, MRC, Microbiol Sect, London, England
Recommended Citation
GB/T 7714
Xie, Hai-Xia,Lu, Jin-Fang,Rolhion, Nathalie,et al. Edwardsiella tarda-Induced Cytotoxicity Depends on Its Type III Secretion System and Flagellin[J]. INFECTION AND IMMUNITY,2014,82(8):3436-3445.
APA Xie, Hai-Xia.,Lu, Jin-Fang.,Rolhion, Nathalie.,Holden, David W..,Nie, Pin.,...&Yu, Xiu-Jun.(2014).Edwardsiella tarda-Induced Cytotoxicity Depends on Its Type III Secretion System and Flagellin.INFECTION AND IMMUNITY,82(8),3436-3445.
MLA Xie, Hai-Xia,et al."Edwardsiella tarda-Induced Cytotoxicity Depends on Its Type III Secretion System and Flagellin".INFECTION AND IMMUNITY 82.8(2014):3436-3445.
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