| Sequence analysis and subcellular localization of crucian carp Carassius auratus viperin | |
| Wang, Bing; Zhang, Yi-Bing; Liu, Ting-Kai; Gui, Jian-Fang | |
| 2014-08-01 | |
| Source Publication | FISH & SHELLFISH IMMUNOLOGY
 |
| ISSN | 1050-4648 |
| Volume | 39Issue:2Pages:168-177 |
| Abstract | Human viperin is known as an interferon (IFN)-inducible antiviral protein and localizes to endoplasmic reticulum (ER) via its N-terminal amphipathic alpha-helix. Little is known about subcellular localization of fish viperin. Herein, we characterized subcellular localization of a fish viperin from crucian carp Carassius auratus. Crucian carp viperin is nearly identical to the other viperin proteins in sequence, with the exception of the first N-terminal 70 amino acids that are defined as N-terminal variable domain including an amphipathic alpha-helix. In addition to N-terminal variable domain, crucian carp viperin protein harbors a conserved middle radical SAM domain and a conserved C-terminal domain. Subcellular localization analyses indicate that crucian carp viperin is a cytoplasmic protein associated with ER. Sequence analyses reveal that amino acids 1-74 forms an amphipathic alpha-helix domain that drives ER-localization of crucian carp viperin. In addition, Coimmunoprecipitation assays show that crucian carp viperin proteins are able to self-associate. These results together indicate that similar to mammalian homologs, fish viperins likely play important roles in IFN response. (C) 2014 Elsevier Ltd. All rights reserved. |
| Subtype | Article |
| Keyword | Viperin Carassius Auratus Cytoplasmic Localization Endoplasmic Reticulum Localization Self-association |
| DOI | 10.1016/j.fsi.2014.04.025 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS Subject | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS ID | WOS:000339773000007 |
| WOS Keyword | INDUCIBLE ANTIVIRAL PROTEIN ; AMPHIPATHIC ALPHA-HELIX ; HEPATITIS-C VIRUS ; HUMAN CYTOMEGALOVIRUS ; MOLECULAR-CLONING ; FISH ; GENE ; REPLICATION ; IDENTIFICATION ; EVOLUTION |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/20347 |
| Collection | 鱼类生物学及渔业生物技术研究中心_期刊论文 |
| Affiliation | Univ Chinese Acad Sci, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| Recommended Citation GB/T 7714 | Wang, Bing,Zhang, Yi-Bing,Liu, Ting-Kai,et al. Sequence analysis and subcellular localization of crucian carp Carassius auratus viperin[J]. FISH & SHELLFISH IMMUNOLOGY,2014,39(2):168-177. |
| APA | Wang, Bing,Zhang, Yi-Bing,Liu, Ting-Kai,&Gui, Jian-Fang.(2014).Sequence analysis and subcellular localization of crucian carp Carassius auratus viperin.FISH & SHELLFISH IMMUNOLOGY,39(2),168-177. |
| MLA | Wang, Bing,et al."Sequence analysis and subcellular localization of crucian carp Carassius auratus viperin".FISH & SHELLFISH IMMUNOLOGY 39.2(2014):168-177. |
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