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题名: Sequence analysis and subcellular localization of crucian carp Carassius auratus viperin
作者: Wang, Bing1; Zhang, Yi-Bing1; Liu, Ting-Kai1; Gui, Jian-Fang1
关键词: Viperin ; Carassius auratus ; Cytoplasmic localization ; Endoplasmic reticulum localization ; Self-association
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2014-08-01
DOI: 10.1016/j.fsi.2014.04.025
卷: 39, 期:2, 页:168-177
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
英文摘要: Human viperin is known as an interferon (IFN)-inducible antiviral protein and localizes to endoplasmic reticulum (ER) via its N-terminal amphipathic alpha-helix. Little is known about subcellular localization of fish viperin. Herein, we characterized subcellular localization of a fish viperin from crucian carp Carassius auratus. Crucian carp viperin is nearly identical to the other viperin proteins in sequence, with the exception of the first N-terminal 70 amino acids that are defined as N-terminal variable domain including an amphipathic alpha-helix. In addition to N-terminal variable domain, crucian carp viperin protein harbors a conserved middle radical SAM domain and a conserved C-terminal domain. Subcellular localization analyses indicate that crucian carp viperin is a cytoplasmic protein associated with ER. Sequence analyses reveal that amino acids 1-74 forms an amphipathic alpha-helix domain that drives ER-localization of crucian carp viperin. In addition, Coimmunoprecipitation assays show that crucian carp viperin proteins are able to self-associate. These results together indicate that similar to mammalian homologs, fish viperins likely play important roles in IFN response. (C) 2014 Elsevier Ltd. All rights reserved.
关键词[WOS]: INDUCIBLE ANTIVIRAL PROTEIN ; AMPHIPATHIC ALPHA-HELIX ; HEPATITIS-C VIRUS ; HUMAN CYTOMEGALOVIRUS ; MOLECULAR-CLONING ; FISH ; GENE ; REPLICATION ; IDENTIFICATION ; EVOLUTION
语种: 英语
WOS记录号: WOS:000339773000007
ISSN号: 1050-4648
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/20347
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Univ Chinese Acad Sci, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

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Wang, Bing; Zhang, Yi-Bing; Liu, Ting-Kai; Gui, Jian-Fang.Sequence analysis and subcellular localization of crucian carp Carassius auratus viperin,FISH & SHELLFISH IMMUNOLOGY,2014,():
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