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A highly effective TALEN-mediated approach for targeted gene disruption in Xenopus tropicalis and zebrafish
Liu, Yun1,4; Luo, Daji2,3; Lei, Yong1,4; Hu, Wei2; Zhao, Hui1,4; Cheng, Christopher H. K.1,4
2014-08-15
Source PublicationMETHODS
ISSN1046-2023
Volume69Issue:1Pages:58-66
AbstractTranscription activator like effector nucleases (TALENs) is a promising approach to disrupt intended genomic loci. The assembly of highly effective TALENs is critical for successful genome editing. Recently we reported a convenient and robust platform to construct customized TALENs. The TALENs generated by this platform have been proven to be highly effective for gene disruption in Xenopus tropicalis and zebrafish as well as large genomic deletions in zebrafish. The one-time success rate of targeted gene disruption is about 90% for more than 100 genomic loci tested, with the mutation frequencies often reaching above 50%. Here we describe the validated protocol for TALEN assembly, methods for generating gene knockout animals in X. tropicalis and zebrafish, as well as the protocol for engineering large genomic deletions in zebrafish. (C) 2014 Elsevier Inc. All rights reserved.
SubtypeArticle
KeywordTalens Gene Knockout Genomic Deletions Xenopus Zebrafish
DOI10.1016/j.ymeth.2014.02.011
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemical Research Methods ; Biochemistry & Molecular Biology
WOS IDWOS:000341803500009
WOS KeywordZINC-FINGER NUCLEASES ; CHROMOSOMAL DELETIONS ; GENOME ; INVERSIONS ; EMBRYOS ; SYSTEM ; GENERATION ; CRISPR/CAS ; TOOLBOX ; CAS9
Citation statistics
Cited Times:38[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20346
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Affiliation1.Chinese Univ Hong Kong, Sch Biomed Sci, Shatin, Hong Kong, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
3.Wuhan Univ, Sch Basic Med Sci, Dept Genet, Wuhan 430072, Peoples R China
4.Chinese Univ Hong Kong, Shenzhen Res Inst, Sch Biomed Sci, Core Lab, Shenzhen 518057, Peoples R China
Recommended Citation
GB/T 7714
Liu, Yun,Luo, Daji,Lei, Yong,et al. A highly effective TALEN-mediated approach for targeted gene disruption in Xenopus tropicalis and zebrafish[J]. METHODS,2014,69(1):58-66.
APA Liu, Yun,Luo, Daji,Lei, Yong,Hu, Wei,Zhao, Hui,&Cheng, Christopher H. K..(2014).A highly effective TALEN-mediated approach for targeted gene disruption in Xenopus tropicalis and zebrafish.METHODS,69(1),58-66.
MLA Liu, Yun,et al."A highly effective TALEN-mediated approach for targeted gene disruption in Xenopus tropicalis and zebrafish".METHODS 69.1(2014):58-66.
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