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Development of an Ussuri catfish Pseudobagrus ussuriensis skin cell line displaying differential cytopathic effects to three aquatic animal viruses
Ou, Tong1; Lei, Xiao-Ying1; He, Li-Bo1; Zhou, Feng-Jian2; Zhang, Qi-Ya1
2014-08-30
Source PublicationVIRUS RESEARCH
ISSN0168-1702
Volume189Pages:56-62
AbstractAn Ussuri catfish Pseudobagrus ussuriensis skin (UCS) cell line was developed and subcultured for more than 60 passages. UCS cells consisted of mostly epithelial-like cells and multiplied well in TC199 medium supplemented with 10% fetal bovine serum at 25 degrees C. Chromosome analysis revealed that most UCS cells had a normal diploid karyotype with 2n = 52. UCS cells showed differential cytopathic effects (CPEs) after inoculation of spring viremia of carp virus (SVCV, a negative-strand RNA virus), grass carp reovirus (GCRV, a multi-segmented double-stranded RNA virus) and Rana grylio virus (RGV, a large double-stranded DNA virus), and were indicative of high sensitivities to these three aquatic animal viruses by a virus titration study. The CPE caused by SVCV appeared as rounded and granular cells, grape-like clusters and small lytic plaques. Characteristic CPE containing plaque-like syncytia was induced by GCRV. RGV-infected cells produced typical CPE characterized by cells shrinkage and aggregation, formation of clear plaques and cell sheet detachment. Furthermore, significant fluorescent signals were observed after UCS cells were transfected with green fluorescent protein reporter plasmids, and the development of CPE induced by a recombinant RGV, Delta TK-RGV, in UCS cells was illustrated using a combination of light and fluorescence microscopy. The data from this study suggested that UCS cell line can potentially serve as a useful tool for the comparison study of different aquatic animal viruses and the isolation of some newly emerging viruses in Ussuri catfish farming. (C) 2014 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordUssuri Catfish Skin (Ucs) Cell Line Cytopathic Effect (Cpe) Spring Viremia Of Carp Virus (Svcv) Grass Carp Reovirus (Gcrv) Rana Grylio Virus (Rgv) Recombinant Iridovirus
DOI10.1016/j.virusres.2014.04.013
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaVirology
WOS SubjectVirology
WOS IDWOS:000341557700009
WOS KeywordRANA-GRYLIO VIRUS ; COMPLETE GENOME SEQUENCE ; RED-SEA BREAM ; CYPRINUS-CARPIO ; SPRING VIREMIA ; TELEOST FISH ; B-CELLS ; RHABDOVIRUS ; ESTABLISHMENT ; REOVIRUS
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Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20344
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Huaian Fisheries Sci Res Inst Jiangsu Prov, Huaian 223001, Peoples R China
Recommended Citation
GB/T 7714
Ou, Tong,Lei, Xiao-Ying,He, Li-Bo,et al. Development of an Ussuri catfish Pseudobagrus ussuriensis skin cell line displaying differential cytopathic effects to three aquatic animal viruses[J]. VIRUS RESEARCH,2014,189:56-62.
APA Ou, Tong,Lei, Xiao-Ying,He, Li-Bo,Zhou, Feng-Jian,&Zhang, Qi-Ya.(2014).Development of an Ussuri catfish Pseudobagrus ussuriensis skin cell line displaying differential cytopathic effects to three aquatic animal viruses.VIRUS RESEARCH,189,56-62.
MLA Ou, Tong,et al."Development of an Ussuri catfish Pseudobagrus ussuriensis skin cell line displaying differential cytopathic effects to three aquatic animal viruses".VIRUS RESEARCH 189(2014):56-62.
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