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Sex-Biased miRNAs in Gonad and Their Potential Roles for Testis Development in Yellow Catfish
Jing, Jing1; Wu, Junjie1; Liu, Wei2; Xiong, Shuting1; Ma, Wenge1; Zhang, Jin1; Wang, Weimin1; Gui, Jian-Fang1,2; Mei, Jie1
2014-09-17
Source PublicationPLOS ONE
ISSN1932-6203
Volume9Issue:9
AbstractRecently, YY super-male yellow catfish had been created by hormonal-induced sex reversal and sex-linked markers, which provides a promising research model for fish sex differentiation and gonad development, especially for testis development. MicroRNAs (miRNAs) have been revealed to play crucial roles in the gene regulation and gonad development in vertebrates. In this study, three small RNA libraries constructed from gonad tissues of XX female, XY male and YY super-male yellow catfish were sequenced. The sequencing data generated a total of 384 conserved miRNAs and 113 potential novel miRNAs, among which 23, 30 and 14 miRNAs were specifically detected in XX ovary, XY testis, and YY testis, respectively. We observed relative lower expression of several miR-200 family members, including miR-141 and miR-429 in YY testis compared with XY testis. Histological analysis indicated a higher degree of testis maturity in YY super-males compared with XY males, as shown by larger spermatogenic cyst, more spermatids and fewer spermatocytes in the spermatogenic cyst. Moreover, five miR-200 family members were significantly up-regulated in testis when treated by 17 alpha-ethinylestradiol (EE2), high dose of which will impair testis development and cell proliferation. The down-regulation of miR-141 and 429 coincides with the progression of testis development in both yellow catfish and human. At last, the expression pattern of nine arbitrarily selected miRNAs detected by quantitative RT-PCR was consistent with the Solexa sequencing results. Our study provides a comprehensive miRNA transcriptome analysis for gonad of yellow catfish with different sex genotypes, and identifies a number of sex-biased miRNAs, some of that are potentially involved in testis development and spermatogenesis.
SubtypeArticle
KeywordPelteobagrus-fulvidraco Richardson Halibut Hippoglossus-hippoglossus Germline Messenger-rnas Microrna Expression Carassius-auratus Zebrafish Mir-430 Cell Development Granulosa-cells Differentiation Growth
DOI10.1371/journal.pone.0107946
WOS HeadingsScience & Technology
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000342123900089
WOS KeywordPELTEOBAGRUS-FULVIDRACO RICHARDSON ; HALIBUT HIPPOGLOSSUS-HIPPOGLOSSUS ; GERMLINE MESSENGER-RNAS ; MICRORNA EXPRESSION ; CARASSIUS-AURATUS ; ZEBRAFISH MIR-430 ; CELL DEVELOPMENT ; GRANULOSA-CELLS ; DIFFERENTIATION ; GROWTH
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Cited Times:44[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20337
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Affiliation1.Huazhong Agr Univ, Coll Fisheries, Minist Agr, Key Lab Freshwater Anim Breeding,Freshwater Aquac, Wuhan, Peoples R China
2.Chinese Acad Sci, Univ Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
Recommended Citation
GB/T 7714
Jing, Jing,Wu, Junjie,Liu, Wei,et al. Sex-Biased miRNAs in Gonad and Their Potential Roles for Testis Development in Yellow Catfish[J]. PLOS ONE,2014,9(9).
APA Jing, Jing.,Wu, Junjie.,Liu, Wei.,Xiong, Shuting.,Ma, Wenge.,...&Mei, Jie.(2014).Sex-Biased miRNAs in Gonad and Their Potential Roles for Testis Development in Yellow Catfish.PLOS ONE,9(9).
MLA Jing, Jing,et al."Sex-Biased miRNAs in Gonad and Their Potential Roles for Testis Development in Yellow Catfish".PLOS ONE 9.9(2014).
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