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题名: Sex-Biased miRNAs in Gonad and Their Potential Roles for Testis Development in Yellow Catfish
作者: Jing, Jing1; Wu, Junjie1; Liu, Wei2; Xiong, Shuting1; Ma, Wenge1; Zhang, Jin1; Wang, Weimin1; Gui, Jian-Fang1, 2; Mei, Jie1
关键词: PELTEOBAGRUS-FULVIDRACO RICHARDSON ; HALIBUT HIPPOGLOSSUS-HIPPOGLOSSUS ; GERMLINE MESSENGER-RNAS ; MICRORNA EXPRESSION ; CARASSIUS-AURATUS ; ZEBRAFISH MIR-430 ; CELL DEVELOPMENT ; GRANULOSA-CELLS ; DIFFERENTIATION ; GROWTH
刊名: PLOS ONE
发表日期: 2014-09-17
DOI: 10.1371/journal.pone.0107946
卷: 9, 期:9
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
英文摘要: Recently, YY super-male yellow catfish had been created by hormonal-induced sex reversal and sex-linked markers, which provides a promising research model for fish sex differentiation and gonad development, especially for testis development. MicroRNAs (miRNAs) have been revealed to play crucial roles in the gene regulation and gonad development in vertebrates. In this study, three small RNA libraries constructed from gonad tissues of XX female, XY male and YY super-male yellow catfish were sequenced. The sequencing data generated a total of 384 conserved miRNAs and 113 potential novel miRNAs, among which 23, 30 and 14 miRNAs were specifically detected in XX ovary, XY testis, and YY testis, respectively. We observed relative lower expression of several miR-200 family members, including miR-141 and miR-429 in YY testis compared with XY testis. Histological analysis indicated a higher degree of testis maturity in YY super-males compared with XY males, as shown by larger spermatogenic cyst, more spermatids and fewer spermatocytes in the spermatogenic cyst. Moreover, five miR-200 family members were significantly up-regulated in testis when treated by 17 alpha-ethinylestradiol (EE2), high dose of which will impair testis development and cell proliferation. The down-regulation of miR-141 and 429 coincides with the progression of testis development in both yellow catfish and human. At last, the expression pattern of nine arbitrarily selected miRNAs detected by quantitative RT-PCR was consistent with the Solexa sequencing results. Our study provides a comprehensive miRNA transcriptome analysis for gonad of yellow catfish with different sex genotypes, and identifies a number of sex-biased miRNAs, some of that are potentially involved in testis development and spermatogenesis.
关键词[WOS]: PELTEOBAGRUS-FULVIDRACO RICHARDSON ; HALIBUT HIPPOGLOSSUS-HIPPOGLOSSUS ; GERMLINE MESSENGER-RNAS ; MICRORNA EXPRESSION ; CARASSIUS-AURATUS ; ZEBRAFISH MIR-430 ; CELL DEVELOPMENT ; GRANULOSA-CELLS ; DIFFERENTIATION ; GROWTH
语种: 英语
WOS记录号: WOS:000342123900089
ISSN号: 1932-6203
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/20337
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Huazhong Agr Univ, Coll Fisheries, Minist Agr, Key Lab Freshwater Anim Breeding,Freshwater Aquac, Wuhan, Peoples R China
2.Chinese Acad Sci, Univ Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China

Recommended Citation:
Jing, Jing; Wu, Junjie; Liu, Wei; Xiong, Shuting; Ma, Wenge; Zhang, Jin; Wang, Weimin; Gui, Jian-Fang; Mei, Jie.Sex-Biased miRNAs in Gonad and Their Potential Roles for Testis Development in Yellow Catfish,PLOS ONE,2014,():
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