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Double Transgenesis of Humanized fat1 and fat2 Genes Promotes Omega-3 Polyunsaturated Fatty Acids Synthesis in a Zebrafish Model
Pang, Shao-Chen1,4; Wang, Hou-Peng1; Li, Kuo-Yu1; Zhu, Zuo-Yan1; Kang, Jing X.2,3; Sun, Yong-Hua1
2014-10-01
Source PublicationMARINE BIOTECHNOLOGY
ISSN1436-2228
Volume16Issue:5Pages:580-593
AbstractOmega-3 long-chain polyunsaturated fatty acid (n-3 LC-PUFA), especially eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), are essential nutrients for human health. However, vertebrates, including humans, have lost the abilities to synthesize EPA and DHA de novo, majorly due to the genetic absence of delta-12 desaturase and omega-3 desaturase genes. Fishes, especially those naturally growing marine fish, are major dietary source of EPA and DHA. Because of the severe decline of marine fishery and the decrease in n-3 LC-PUFA content of farmed fishes, it is highly necessary to develop alternative sources of n-3 LC-PUFA. In the present study, we utilized transgenic technology to generate n-3 LC-PUFA-rich fish by using zebrafish as an animal model. Firstly, fat1 was proved to function efficiently in fish culture cells, which showed an effective conversion of n-6 PUFA to n-3 PUFA with the n-6/n-3 ratio that decreased from 7.7 to 1.1. Secondly, expression of fat1 in transgenic zebrafish increased the 20:5n-3 and 22:6n-3 contents to 1.8- and 2.4-fold, respectively. Third, co-expression of fat2, a fish codon-optimized delta-12 desaturase gene, and fat1 in fish culture cell significantly promoted n-3 PUFA synthesis with the decreased n-6/n-3 ratio from 7.7 to 0.7. Finally, co-expression of fat1 and fat2 in double transgenic zebrafish increased the 20:5n-3 and 22:6n-3 contents to 1.7- and 2.8-fold, respectively. Overall, we generated two types of transgenic zebrafish rich in endogenous n-3 LC-PUFA, fat1 transgenic zebrafish and fat1/fat2 double transgenic zebrafish. Our results demonstrate that application of transgenic technology of humanized fat1 and fat2 in farmed fishes can largely improve the n-3 LC-PUFA production.
SubtypeArticle
KeywordOmega-3 Fatty Acids Omega-6 Fatty Acids Desaturase Transgenic Fish Transcriptional Regulation
DOI10.1007/s10126-014-9577-9
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS SubjectBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS IDWOS:000341504200009
WOS KeywordSALMON SALMO-SALAR ; FISH-OIL ; HETEROLOGOUS EXPRESSION ; BIOSYNTHETIC-PATHWAY ; MAMMALIAN-CELLS ; DESATURASE GENE ; VEGETABLE-OIL ; N-3 ; MICE ; GENERATION
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20334
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Massachusetts Gen Hosp, Dept Med, Lab Lipid Med & Technol, Boston, MA 02114 USA
3.Harvard Univ, Sch Med, Boston, MA USA
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Pang, Shao-Chen,Wang, Hou-Peng,Li, Kuo-Yu,et al. Double Transgenesis of Humanized fat1 and fat2 Genes Promotes Omega-3 Polyunsaturated Fatty Acids Synthesis in a Zebrafish Model[J]. MARINE BIOTECHNOLOGY,2014,16(5):580-593.
APA Pang, Shao-Chen,Wang, Hou-Peng,Li, Kuo-Yu,Zhu, Zuo-Yan,Kang, Jing X.,&Sun, Yong-Hua.(2014).Double Transgenesis of Humanized fat1 and fat2 Genes Promotes Omega-3 Polyunsaturated Fatty Acids Synthesis in a Zebrafish Model.MARINE BIOTECHNOLOGY,16(5),580-593.
MLA Pang, Shao-Chen,et al."Double Transgenesis of Humanized fat1 and fat2 Genes Promotes Omega-3 Polyunsaturated Fatty Acids Synthesis in a Zebrafish Model".MARINE BIOTECHNOLOGY 16.5(2014):580-593.
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