IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Molecular cloning and characterization of cathepsin L from freshwater mussel, Cristaria plicata
Hu, Xiaojuan1; Hu, Xiangping1; Hu, Baoqing1; Wen, Chungen1; Xie, Yanhai1; Wu, Dan1; Tao, Zhiying1; Li, Aihua2; Gao, Qian2
2014-10-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume40Issue:2Pages:446-454
AbstractCathepsin L is one of the crucial enzyme superfamilies and involved in the immune responses. The Cathepsin L cDNA and genome of Cristaria plicata (CpCL) was cloned from the hemocytes using degenerate primers by the rapid amplification of cDNA ends (RACE) PCR. The genomic DNA was 9353 bp long and had a total of six introns and seven exons. The full-length cDNA of CpCL was 1144 bp, the cDNA contained a 5' untranslated region (UTR) of 34 nucleotides, the 3' UTR of 108 bp with a canonical polyadenylation signal sequence AATAAA and a polyA tail, and an open reading frame (ORF) of 1002 bp, encoding 333 amino acid residues with 37.65 kDa predicted molecular weight. The theoretical isoelectric point was 8.61. The prepro-cathepsin L was consisted of a typical signal peptide (Met1-Gly20), a pro-region peptide (Leu21-Glu116) and a mature peptide (Tyr117-Val333). Many members of the papain family possessed of a proline residue at position 2 in the mature enzymem, this was also observed in CpCL. The preproprotein included an oxyanion hole (Gln 135), the active center formed by Cys141, His280 and Asn 300, the potential N-glycosylation site (Asn38, Asn 113 and Asn 272) and the conserved GCXGG motifs, which was characteristic of cathepsin, the conserved ERWNIN and GNFD motifs, which were characteristic for cathepsin L. Homology analysis revealed that the CpCL shared 49-87% identity to other known cathepsin L sequences. The phylogenetic tree showed that the CpCL clustered with the invertebrate cathepsin L cysteine proteases, and was closely related to the cathepsin L of Hyriopsis cumingii. The expression of CpCL mRNA was detected in hepatopancreas, hemocytes, mantle, gills and adductor muscle, Mid the higher expression level was in hepatopancreas. After A. hydrophila stimulation, the expression of the CpCL mRNA was up-regulated in hemocytes and hepatopancreas, and the expression level was significantly lower in gill than one after PBS challenge group. (C) 2014 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordCristaria Plicata Cathepsin l Molecular Clone Genome Expression
DOI10.1016/j.fsi.2014.07.005
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000343381100013
WOS KeywordCYSTEINE PROTEASES ; GENE-EXPRESSION ; CAENORHABDITIS-ELEGANS ; FUNCTIONAL EXPRESSION ; SCHISTOSOMA-MANSONI ; FASCIOLA-HEPATICA ; PROTEINASES ; EMBRYOGENESIS ; OYSTER ; IDENTIFICATION
Citation statistics
Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20332
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Affiliation1.Nanchang Univ, Sch Life Sci & Food Engn, Inst Life Sci, Nanchang 330031, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
Recommended Citation
GB/T 7714
Hu, Xiaojuan,Hu, Xiangping,Hu, Baoqing,et al. Molecular cloning and characterization of cathepsin L from freshwater mussel, Cristaria plicata[J]. FISH & SHELLFISH IMMUNOLOGY,2014,40(2):446-454.
APA Hu, Xiaojuan.,Hu, Xiangping.,Hu, Baoqing.,Wen, Chungen.,Xie, Yanhai.,...&Gao, Qian.(2014).Molecular cloning and characterization of cathepsin L from freshwater mussel, Cristaria plicata.FISH & SHELLFISH IMMUNOLOGY,40(2),446-454.
MLA Hu, Xiaojuan,et al."Molecular cloning and characterization of cathepsin L from freshwater mussel, Cristaria plicata".FISH & SHELLFISH IMMUNOLOGY 40.2(2014):446-454.
Files in This Item:
File Name/Size DocType Version Access License
Molecular cloning an(2749KB) 开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Hu, Xiaojuan]'s Articles
[Hu, Xiangping]'s Articles
[Hu, Baoqing]'s Articles
Baidu academic
Similar articles in Baidu academic
[Hu, Xiaojuan]'s Articles
[Hu, Xiangping]'s Articles
[Hu, Baoqing]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Hu, Xiaojuan]'s Articles
[Hu, Xiangping]'s Articles
[Hu, Baoqing]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Molecular cloning and characterization of cathepsin L from freshwater mussel, Cristaria plicata.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.