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题名: Characterization and Development of EST-SSR Markers Derived from Transcriptome of Yellow Catfish
作者: Zhang, Jin1; Ma, Wenge1; Song, Xiaomin1; Lin, Qiaohong1; Gui, Jian-Fang1, 2; Mei, Jie1
关键词: EST-SSRs ; yellow catfish ; 454 pyrosequencing ; genetic diversity
刊名: MOLECULES
发表日期: 2014-10-01
DOI: 10.3390/molecules191016402
卷: 19, 期:10, 页:16402-16415
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Physical Sciences
类目[WOS]: Chemistry, Organic
研究领域[WOS]: Chemistry
英文摘要: Yellow catfish (Pelteobagrus fulvidraco) is one of the most important freshwater fish due to its delicious flesh and high nutritional value. However, lack of sufficient simple sequence repeat (SSR) markers has hampered the progress of genetic selection breeding and molecular research for yellow catfish. To this end, we aimed to develop and characterize polymorphic expressed sequence tag (EST)-SSRs from the 454 pyrosequencing transcriptome of yellow catfish. Totally, 82,794 potential EST-SSR markers were identified and distributed in the coding and non-coding regions. Di-nucleotide (53,933) is the most abundant motif type, and AC/GT, AAT/ATT, AAAT/ATTT are respective the most frequent di-, tri-, tetra-nucleotide repeats. We designed primer pairs for all of the identified EST-SSRs and randomly selected 300 of these pairs for further validation. Finally, 263 primer pairs were successfully amplified and 57 primer pairs were found to be consistently polymorphic when four populations of 48 individuals were tested. The number of alleles for the 57 loci ranged from 2 to 17, with an average of 8.23. The observed heterozygosity (HO), expected heterozygosity (HE), polymorphism information content (PIC) and fixation index (FIS) values ranged from 0.04 to 1.00, 0.12 to 0.92, 0.12 to 0.91 and -0.83 to 0.93, respectively. These EST-SSR markers generated in this study could greatly facilitate future studies of genetic diversity and molecular breeding in yellow catfish.
关键词[WOS]: LINKAGE MAP ; IDENTIFICATION ; LENGTH ; ISSR
语种: 英语
WOS记录号: WOS:000344456100058
ISSN号: 1420-3049
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/20331
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Huazhong Agr Univ, Freshwater Aquaculture Collaborat Innovat Ctr Hub, Coll Fisheries, Key Lab Freshwater Anim Breeding,Minist Agr, Wuhan 430070, Peoples R China
2.Univ Chinese Acad Sci, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

Recommended Citation:
Zhang, Jin; Ma, Wenge; Song, Xiaomin; Lin, Qiaohong; Gui, Jian-Fang; Mei, Jie.Characterization and Development of EST-SSR Markers Derived from Transcriptome of Yellow Catfish,MOLECULES,2014,():
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