| Fish viperin exerts a conserved antiviral function through RLR-triggered IFN signaling pathway | |
| Wang, Bing; Zhang, Yi-Bing; Liu, Ting-Kai; Shi, Jun; Sun, Fan; Gui, Jian-Fang | |
| 2014-11-01 | |
| Source Publication | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| ISSN | 0145-305X |
| Volume | 47Issue:1Pages:140-149 |
| Abstract | Mammalian viperin is a typical interferon (IFN)-induced antiviral protein. Fish have viperin homologs; however, little is known about the expression regulation of fish viperins. In this study, we report the expression regulation and antiviral function of a fish viperin from crucian carp Carassius auratus during IFN response. Crucian carp viperin is induced at mRNA and protein levels by fish IFNs and IFN stimuli such as poly(I:C). Consistently, this gene promoter contains multiple transcription factor binding sites including IFN-stimulated response elements (ISRE) and IFN gamma activation sequences (GAS), and is activated by two types of fish IFNs and also by the intracellular and extracellular poly(I:C). Activation of crucian carp viperin promoter by the intracellular poly(I:C) is mediated by retinoic acid-inducing gene I (RIG-I)-like receptors (RLR)-triggered IFN signaling pathway, which is further verified by the findings that each signaling molecule of RLR pathway is able to induce the expression of crucian carp viperin at mRNA and protein levels. Finally, overexpression of crucian carp viperin in cultured fish cells confers significant protection against infection of grass carp reovirus (GCRV). These data suggest that similar to mammalian homologs, crucian carp viperin exerts a conserved function through RLR-triggered IFN signaling pathway. (C) 2014 Elsevier Ltd. All rights reserved. |
| Subtype | Article |
| Keyword | Viperin Carassius Auratus Ifn Inducible Protein Promoter Analysis Rlr Signaling Pathway Antiviral Function |
| DOI | 10.1016/j.dci.2014.07.006 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Immunology ; Zoology |
| WOS Subject | Immunology ; Zoology |
| WOS ID | WOS:000342275400017 |
| WOS Keyword | INNATE IMMUNE-RESPONSE ; CARP CARASSIUS-AURATUS ; TROUT ONCORHYNCHUS-MYKISS ; DEPENDENT PROTEIN-KINASE ; HEPATITIS-C VIRUS ; GENE-EXPRESSION ; SUBCELLULAR-LOCALIZATION ; HUMAN CYTOMEGALOVIRUS ; MOLECULAR-CLONING ; DENDRITIC CELLS |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/20330 |
| Collection | 鱼类生物学及渔业生物技术研究中心_期刊论文 |
| Affiliation | Univ Chinese Acad Sci, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| Recommended Citation GB/T 7714 | Wang, Bing,Zhang, Yi-Bing,Liu, Ting-Kai,et al. Fish viperin exerts a conserved antiviral function through RLR-triggered IFN signaling pathway[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2014,47(1):140-149. |
| APA | Wang, Bing,Zhang, Yi-Bing,Liu, Ting-Kai,Shi, Jun,Sun, Fan,&Gui, Jian-Fang.(2014).Fish viperin exerts a conserved antiviral function through RLR-triggered IFN signaling pathway.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,47(1),140-149. |
| MLA | Wang, Bing,et al."Fish viperin exerts a conserved antiviral function through RLR-triggered IFN signaling pathway".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 47.1(2014):140-149. |
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