中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
题名: Development of a multiplex PCR assay for rapid and simultaneous detection of four genera of fish pathogenic bacteria
作者: Zhang, D. F.1, 2; Zhang, Q. Q.2; Li, A. H.2
关键词: aquaculture ; detection ; fish pathogenic bacteria ; genus-specific primers ; multiplex PCR
刊名: LETTERS IN APPLIED MICROBIOLOGY
发表日期: 2014-11-01
DOI: 10.1111/lam.12303
卷: 59, 期:5, 页:471-478
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology ; Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology ; Microbiology
英文摘要: Species of genus Aeromonas, Vibrio, Edwardsiella and Streptococcus are the most common fish pathogenic bacteria that cause economically devastating losses in aquaculture. A multiplex polymerase chain reaction (mPCR) was developed for the simultaneous detection and differentiation of the four genera of fish pathogenic bacteria. Through the use of genus-specific primers instead of species-specific ones, the current mPCR covered much more target bacterial species compared with previously reported species-specific mPCR methods. The specificity of the four putative genus-specific primers was validated experimentally while used exclusively (uniplex PCR) or combined (mPCR) against bacterial genomic DNA templates of the target bacteria and nontarget bacteria. The PCR amplicons for the following genera were obtained as expected: Aeromonas (875bp), Vibrio (524bp), Edwardsiella (302bp) and Streptococcus (197bp), and the fragments could be separated clearly on the agarose gel electrophoresis. The mPCR did not produce nonspecific amplification products when used to amplify 21 nontarget species of bacteria. The mPCR detection limits for each target bacterial genera were 50 colony-forming units (CFU) in pure culture and 100CFU in fish tissue samples. In conclusion, the mPCR assay was proven to be a powerful alternative to the conventional culture-based method, given its rapid, specific, sensitive and reliable detection of target pathogens.
关键词[WOS]: POLYMERASE-CHAIN-REACTION ; STREPTOCOCCUS-AGALACTIAE ; EDWARDSIELLA-ICTALURI ; IN-VITRO ; AEROMONAS ; CHINA ; SEQUENCES ; STRAINS ; TILAPIA ; TARDA
语种: 英语
WOS记录号: WOS:000343871400003
ISSN号: 0266-8254
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/20329
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Development of a multiplex PCR assay for rapid and simultaneous detection of four genera of fish pathogenic bacteria.pdf(225KB)----开放获取View Download

作者单位: 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou, Guangdong, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

Recommended Citation:
Zhang, D. F.; Zhang, Q. Q.; Li, A. H..Development of a multiplex PCR assay for rapid and simultaneous detection of four genera of fish pathogenic bacteria,LETTERS IN APPLIED MICROBIOLOGY,2014,():
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhang, D. F.]'s Articles
[Zhang, Q. Q.]'s Articles
[Li, A. H.]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhang, D. F.]‘s Articles
[Zhang, Q. Q.]‘s Articles
[Li, A. H.]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Development of a multiplex PCR assay for rapid and simultaneous detection of four genera of fish pathogenic bacteria.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院水生生物研究所 - Feedback
Powered by CSpace