IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Expression and functional characterization of PGRP6 splice variants in grass carp Ctenopharyngodon idella
Yu, Zhang Long1,2; Li, Jun Hua1; Xue, Na Na1; Nie, Pin1; Chang, Ming Xian1
2014-12-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume47Issue:2Pages:264-274
AbstractPeptidoglycan recognition proteins (PGRPs), which are evolutionarily conserved pattern recognition receptors from insects to mammals, recognize bacterial PGN and function in antibacterial innate immunity. The existence of alternative splicing is a common feature for PGRP family. Here the splicing pattern from the splicing at the 5' end of PGRP6 gene was identified in a teleost fish, the grass carp (Ctenopharyngodon idella). Four splice variants of grass carp PGRP6 were designated as gcPGRP6a, gcPGRP6b, gcPGRP6c and gcPGRP6d, respectively. Real-time PCR revealed the different expression of these variants in fish individuals and CIK cell line in response to stimulation with different microbial ligands. Immunofluorescence microscopy and Western blotting showed that the splice variants are intracellular protein. Cell lysates from Epithelioma papulosum cyprini (EPC) cells transfected with gcPGRP6 splice variants are able to bind microbial PAMPs including Lys-type PGN from Staphylococcus aureus, DAP-type PGN from Bacillus subtilis, glucan, mannan, and microorganisms including Streptococcus dysgalactiae, Flavobacterium columnare and Saccharomyces cerevisiae. Moreover, overexpression of gcPGRP6 variants inhibited earlier stage growth of intracellular bacteria. The data also identified a specific role for gcPGRP6c variant in the positive regulation of cytolytic molecule perforin, and for gcPGRP6a, gcPGRP6b and gcPGRP6c variants in positive regulation of antimicrobial peptides (AMPs). However, the gcPGRP6d variant, which encoded basically only the PGRP domain, failed to induce the expression of perforin and AMPs. It is suggested that fish PGRP6 splice variants have common and variant-specific function in innate immune response. (C) 2014 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordPeptidoglycan Recognition Protein 6 Splice Variants Microbial-binding Activity Antibacterial Activity Grass Carp
DOI10.1016/j.dci.2014.08.006
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaImmunology ; Zoology
WOS SubjectImmunology ; Zoology
WOS IDWOS:000344207800012
WOS KeywordPEPTIDOGLYCAN RECOGNITION PROTEIN ; INNATE IMMUNITY ; MOLECULAR-CLONING ; DROSOPHILA-MELANOGASTER ; NEGATIVE BACTERIA ; CRYSTAL-STRUCTURE ; GENE ; BINDING ; FAMILY ; ZEBRAFISH
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20327
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Yu, Zhang Long,Li, Jun Hua,Xue, Na Na,et al. Expression and functional characterization of PGRP6 splice variants in grass carp Ctenopharyngodon idella[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2014,47(2):264-274.
APA Yu, Zhang Long,Li, Jun Hua,Xue, Na Na,Nie, Pin,&Chang, Ming Xian.(2014).Expression and functional characterization of PGRP6 splice variants in grass carp Ctenopharyngodon idella.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,47(2),264-274.
MLA Yu, Zhang Long,et al."Expression and functional characterization of PGRP6 splice variants in grass carp Ctenopharyngodon idella".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 47.2(2014):264-274.
Files in This Item:
File Name/Size DocType Version Access License
Expression and funct(2942KB) 开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Yu, Zhang Long]'s Articles
[Li, Jun Hua]'s Articles
[Xue, Na Na]'s Articles
Baidu academic
Similar articles in Baidu academic
[Yu, Zhang Long]'s Articles
[Li, Jun Hua]'s Articles
[Xue, Na Na]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Yu, Zhang Long]'s Articles
[Li, Jun Hua]'s Articles
[Xue, Na Na]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Expression and functional characterization of PGRP6 splice variants in grass carp Ctenopharyngodon idella.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.