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题名: Expression and functional characterization of PGRP6 splice variants in grass carp Ctenopharyngodon idella
作者: Yu, Zhang Long1, 2; Li, Jun Hua1; Xue, Na Na1; Nie, Pin1; Chang, Ming Xian1
关键词: Peptidoglycan recognition protein 6 ; Splice variants ; Microbial-binding activity ; Antibacterial activity ; Grass carp
刊名: DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
发表日期: 2014-12-01
DOI: 10.1016/j.dci.2014.08.006
卷: 47, 期:2, 页:264-274
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Immunology ; Zoology
研究领域[WOS]: Immunology ; Zoology
英文摘要: Peptidoglycan recognition proteins (PGRPs), which are evolutionarily conserved pattern recognition receptors from insects to mammals, recognize bacterial PGN and function in antibacterial innate immunity. The existence of alternative splicing is a common feature for PGRP family. Here the splicing pattern from the splicing at the 5' end of PGRP6 gene was identified in a teleost fish, the grass carp (Ctenopharyngodon idella). Four splice variants of grass carp PGRP6 were designated as gcPGRP6a, gcPGRP6b, gcPGRP6c and gcPGRP6d, respectively. Real-time PCR revealed the different expression of these variants in fish individuals and CIK cell line in response to stimulation with different microbial ligands. Immunofluorescence microscopy and Western blotting showed that the splice variants are intracellular protein. Cell lysates from Epithelioma papulosum cyprini (EPC) cells transfected with gcPGRP6 splice variants are able to bind microbial PAMPs including Lys-type PGN from Staphylococcus aureus, DAP-type PGN from Bacillus subtilis, glucan, mannan, and microorganisms including Streptococcus dysgalactiae, Flavobacterium columnare and Saccharomyces cerevisiae. Moreover, overexpression of gcPGRP6 variants inhibited earlier stage growth of intracellular bacteria. The data also identified a specific role for gcPGRP6c variant in the positive regulation of cytolytic molecule perforin, and for gcPGRP6a, gcPGRP6b and gcPGRP6c variants in positive regulation of antimicrobial peptides (AMPs). However, the gcPGRP6d variant, which encoded basically only the PGRP domain, failed to induce the expression of perforin and AMPs. It is suggested that fish PGRP6 splice variants have common and variant-specific function in innate immune response. (C) 2014 Elsevier Ltd. All rights reserved.
关键词[WOS]: PEPTIDOGLYCAN RECOGNITION PROTEIN ; INNATE IMMUNITY ; MOLECULAR-CLONING ; DROSOPHILA-MELANOGASTER ; NEGATIVE BACTERIA ; CRYSTAL-STRUCTURE ; GENE ; BINDING ; FAMILY ; ZEBRAFISH
语种: 英语
WOS记录号: WOS:000344207800012
ISSN号: 0145-305X
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/20327
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China

Recommended Citation:
Yu, Zhang Long; Li, Jun Hua; Xue, Na Na; Nie, Pin; Chang, Ming Xian.Expression and functional characterization of PGRP6 splice variants in grass carp Ctenopharyngodon idella,DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2014,():
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