中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 淡水生态学研究中心  > 期刊论文
题名: Quantitative liquid chromatography-tandem mass spectrometry method for determination of microcystin-RR and its glutathione and cysteine conjugates in fish plasma and bile
作者: Li, Wei1; Xie, Ping1; Chen, Jun1; He, Jun1; Guo, Xiaochun1; Yu, Dezhao1; Chen, Liang1
关键词: Microcystin-RR ; LC-MS/MS ; Metabolites ; Quantification
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2014-07-15
DOI: 10.1016/j.jchromb.2014.05.057
卷: 963, 期:-, 页:113-118
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
英文摘要: A rapid and sensitive liquid chromatography-mass spectrometry (LC-MS) method was developed and validated for the simultaneous determination of microcystin-RR (MC-RR) and its glutathione and cysteine conjugates (MC-RR-GSH and MC-RR-Cys, respectively) in fish plasma and bile. The analytes were extracted using methanol, followed by an Oasis mixed-mode cation-exchange polymeric sorbent. The separation was performed on a reversed-phase Waters XBridge C18 column with the gradient mobile phase, consisting of water and acetonitrile (both acidified with 0.5 parts per thousand formic acid). Mean recoveries of MCRR, MC-RR-GSH and MC-RR-Cys ranged from 80.7 to 93.7%, 81.1 to 93.1% and 80.3 to 93.2%, respectively, at three concentrations (0.2, 1.0 and 5.0 mu g mL(-1)). Limits of detection (LODs) for MC-RR, MC-RR-GSH and MC-RR-Cys were 6, 12 and 9 ng mL(-1), respectively. Limits of quantification (LOQs) were 15,30 and 22.5 ng mL(-1) for MC-RR, MC-RR-GSH and MC-RR-Cys, respectively. This method makes it feasible for the identification and quantification of MC-RR, MC-RR-GSH and MC-RR-Cys in limited and complex biological fluid samples (such as plasma and bile, typically 50111,), which were previously excluded or difficult to study due to the relatively large sample volumes. (C) 2014 Elsevier B.V. All rights reserved.
关键词[WOS]: TROUT ONCORHYNCHUS-MYKISS ; HEPATOTOXIC MICROCYSTINS ; BIGHEAD CARP ; IN-VIVO ; IDENTIFICATION ; CYANOBACTERIA ; METABOLITES ; MS ; BIOACCUMULATION ; DETOXIFICATION
语种: 英语
WOS记录号: WOS:000339131700016
ISSN号: 1570-0232
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/20316
Appears in Collections:淡水生态学研究中心_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Quantitative liquid chromatography-tandem mass spectrometry method for determination of microcystin-RR and its glutathione and cysteine conjugates in fish plasma and bile.pdf(1224KB)----开放获取View Download

作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Li, Wei]'s Articles
[Xie, Ping]'s Articles
[Chen, Jun]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Li, Wei]‘s Articles
[Xie, Ping]‘s Articles
[Chen, Jun]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Quantitative liquid chromatography-tandem mass spectrometry method for determination of microcystin-RR and its glutathione and cysteine conjugates in fish plasma and bile.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院水生生物研究所 - Feedback
Powered by CSpace