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Quantitative liquid chromatography-tandem mass spectrometry method for determination of microcystin-RR and its glutathione and cysteine conjugates in fish plasma and bile
Li, Wei; Xie, Ping; Chen, Jun; He, Jun; Guo, Xiaochun; Yu, Dezhao; Chen, Liang
2014-07-15
Source PublicationJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN1570-0232
Volume963Issue:-Pages:113-118
AbstractA rapid and sensitive liquid chromatography-mass spectrometry (LC-MS) method was developed and validated for the simultaneous determination of microcystin-RR (MC-RR) and its glutathione and cysteine conjugates (MC-RR-GSH and MC-RR-Cys, respectively) in fish plasma and bile. The analytes were extracted using methanol, followed by an Oasis mixed-mode cation-exchange polymeric sorbent. The separation was performed on a reversed-phase Waters XBridge C18 column with the gradient mobile phase, consisting of water and acetonitrile (both acidified with 0.5 parts per thousand formic acid). Mean recoveries of MCRR, MC-RR-GSH and MC-RR-Cys ranged from 80.7 to 93.7%, 81.1 to 93.1% and 80.3 to 93.2%, respectively, at three concentrations (0.2, 1.0 and 5.0 mu g mL(-1)). Limits of detection (LODs) for MC-RR, MC-RR-GSH and MC-RR-Cys were 6, 12 and 9 ng mL(-1), respectively. Limits of quantification (LOQs) were 15,30 and 22.5 ng mL(-1) for MC-RR, MC-RR-GSH and MC-RR-Cys, respectively. This method makes it feasible for the identification and quantification of MC-RR, MC-RR-GSH and MC-RR-Cys in limited and complex biological fluid samples (such as plasma and bile, typically 50111,), which were previously excluded or difficult to study due to the relatively large sample volumes. (C) 2014 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordMicrocystin-rr Lc-ms/ms Metabolites Quantification
DOI10.1016/j.jchromb.2014.05.057
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000339131700016
WOS KeywordTROUT ONCORHYNCHUS-MYKISS ; HEPATOTOXIC MICROCYSTINS ; BIGHEAD CARP ; IN-VIVO ; IDENTIFICATION ; CYANOBACTERIA ; METABOLITES ; MS ; BIOACCUMULATION ; DETOXIFICATION
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20316
Collection淡水生态学研究中心_期刊论文
AffiliationChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Li, Wei,Xie, Ping,Chen, Jun,et al. Quantitative liquid chromatography-tandem mass spectrometry method for determination of microcystin-RR and its glutathione and cysteine conjugates in fish plasma and bile[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2014,963(-):113-118.
APA Li, Wei.,Xie, Ping.,Chen, Jun.,He, Jun.,Guo, Xiaochun.,...&Chen, Liang.(2014).Quantitative liquid chromatography-tandem mass spectrometry method for determination of microcystin-RR and its glutathione and cysteine conjugates in fish plasma and bile.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,963(-),113-118.
MLA Li, Wei,et al."Quantitative liquid chromatography-tandem mass spectrometry method for determination of microcystin-RR and its glutathione and cysteine conjugates in fish plasma and bile".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 963.-(2014):113-118.
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