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题名: Simultaneous quantitative determination of microcystin-LR and its glutathione metabolites in rat liver by liquid chromatography-tandem mass spectrometry
作者: Guo, Xiaochun1; Xie, Ping2; Chen, Jun2; Tuo, Xun2; Deng, Xuwei2; Li, Shangchun2; Yu, Dezhao2; Zeng, Cheng1
关键词: Microcystin-LR ; Microcystin-LR-GSH ; Microcystin-LR-Cys ; LC-ESI-MS/MS ; Quantitative determination ; Rat liver
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2014-07-15
DOI: 10.1016/j.jchromb.2014.05.046
卷: 963, 期:-, 页:54-61
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
英文摘要: The roles of glutathione (GSH) and cysteine (Cys) in the detoxification of Microcystin-LR (MC-LR) have recently become a popular area of research. However, lacking analysis methods for MC-LR-GSH and MC-LR-Cys (two main GSH pathway metabolites) in mammals, elucidation of the detoxification mechanism and metabolic pathway of MC-LR in mammals is difficult. In this study, a novel method for the simultaneous quantitative analysis of MC-LR, MC-LR-GSH and MC-LR-Cys in rat liver was developed and validated. The analytes were simultaneously extracted from rat liver using 3 M sodium chloride solution containing 0.01 M EDTA-Na-2-5% acetic acid, followed by solid-phase extraction (SPE) on Oasis HLB and silica cartridges and determination by liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS/MS). Under the optimized pretreatment conditions and instrument parameters, good recoveries of MC-LR, MC-LR-GSH and MC-LR-Cys were obtained at three concentrations (0.2, 1.0 and 2.5 mu g g(-1) dry weight (DW)) with values ranging from 97.7 +/- 4.2 to 98.7 +/- 5.1%, 70.1 +/- 4.8 to 71.1 +/- 4.1% and 79.8 +/- 3.5 to 81.4 +/- 4.0%, respectively. The relative standard deviations (RSDs) of these compounds at 0.2, 1.0 and 2.5 mu g g(-1) DW were between 4.3% and 6.9%. The limits of detection (LODs) were 0.005, 0.007 and 0.006 mu g g(-1) DW and the limits of quantification (LOQs) were 0,017, 0.023 and 0.020 mu g g(-1) DW for MC-LR, MC-LR-GSH and MC-LR-Cys, respectively. Furthermore, this method was successfully applied to both time- and dosage-effect studies of MC-LR, MC-LR-GSH and MC-LR-Cys in vivo. (C) 2014 Elsevier B.V. All rights reserved.
关键词[WOS]: HEPATOTOXIC MICROCYSTINS ; RISK-ASSESSMENT ; BIGHEAD CARP ; CYANOBACTERIAL TOXINS ; DRINKING-WATER ; TOXICITY ; FISH ; HEALTH ; ASSAY ; MICE
语种: 英语
WOS记录号: WOS:000339131700008
ISSN号: 1570-0232
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/20315
Appears in Collections:淡水生态学研究中心_期刊论文

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作者单位: 1.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Wuhan 430072, Peoples R China
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