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题名: Nanochromosome Copy Number Does not Correlate with RNA Levels Though Patterns are Conserved between Strains of the Ciliate Morphospecies Chilodonella uncinata
作者: Huang, Jie1, 2; Katz, Laura A.1, 3
关键词: Chilodonella ; ciliate ; copy number variation ; RNA levels ; quantitative PCR
刊名: PROTIST
发表日期: 2014-08-01
DOI: 10.1016/j.protis.2014.04.005
卷: 165, 期:4, 页:445-451
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Microbiology
研究领域[WOS]: Microbiology
英文摘要: In some ciliates, extensive genome fragmentation leads to a macronucleus (i.e. somatic nucleus) containing gene-sized chromosomes that vary in copy number. Yet the relationship between copy number and expression level is not well understood as previous work has shown a variety of patterns. For example, nanochromosome copy numbers are positively correlated to mRNA levels in spirotrichous ciliates, while one study of Chilodonella uncinata suggested that they were inversely correlated. To study further copy number and expression levels in C. uncinata, we analyzed 11 members in five gene families (SSU-rDNA, actin, alpha-tubulin, histidine acid phosphatase family protein and a protein kinase domain containing protein) from one strain. We find that macronuclear copy numbers of these genes range from hundreds to thousands per cell, and that copy number does not correlate with expression level as measured by steady-state RNA in predominantly-vegetative cultures. We also compared six of these genes to their orthologs in a second genetically-isolated strain of C. uncinata to reveal that patterns of nanochromosome and transcript copy numbers are conserved between strains. Our data suggest that nanochromosome copy number may be related to a feature like nuclear architecture. (C) 2014 Elsevier GmbH. All rights reserved.
关键词[WOS]: EPIGENETIC REGULATION ; GENE-EXPRESSION ; DNA ; AMPLIFICATION ; MACRONUCLEUS
语种: 英语
WOS记录号: WOS:000340583300003
ISSN号: 1434-4610
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/20312
Appears in Collections:淡水生态学研究中心_期刊论文

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作者单位: 1.Smith Coll, Dept Biol Sci, Northampton, MA 01063 USA
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China
3.UMass Amherst, Program Organism & Evolutionary Biol, Amherst, MA 01003 USA
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