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Nanochromosome Copy Number Does not Correlate with RNA Levels Though Patterns are Conserved between Strains of the Ciliate Morphospecies Chilodonella uncinata
Huang, Jie1,2; Katz, Laura A.1,3
2014-08-01
Source PublicationPROTIST
ISSN1434-4610
Volume165Issue:4Pages:445-451
AbstractIn some ciliates, extensive genome fragmentation leads to a macronucleus (i.e. somatic nucleus) containing gene-sized chromosomes that vary in copy number. Yet the relationship between copy number and expression level is not well understood as previous work has shown a variety of patterns. For example, nanochromosome copy numbers are positively correlated to mRNA levels in spirotrichous ciliates, while one study of Chilodonella uncinata suggested that they were inversely correlated. To study further copy number and expression levels in C. uncinata, we analyzed 11 members in five gene families (SSU-rDNA, actin, alpha-tubulin, histidine acid phosphatase family protein and a protein kinase domain containing protein) from one strain. We find that macronuclear copy numbers of these genes range from hundreds to thousands per cell, and that copy number does not correlate with expression level as measured by steady-state RNA in predominantly-vegetative cultures. We also compared six of these genes to their orthologs in a second genetically-isolated strain of C. uncinata to reveal that patterns of nanochromosome and transcript copy numbers are conserved between strains. Our data suggest that nanochromosome copy number may be related to a feature like nuclear architecture. (C) 2014 Elsevier GmbH. All rights reserved.
SubtypeArticle
KeywordChilodonella Ciliate Copy Number Variation Rna Levels Quantitative Pcr
DOI10.1016/j.protis.2014.04.005
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000340583300003
WOS KeywordEPIGENETIC REGULATION ; GENE-EXPRESSION ; DNA ; AMPLIFICATION ; MACRONUCLEUS
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20312
Collection淡水生态学研究中心_期刊论文
Affiliation1.Smith Coll, Dept Biol Sci, Northampton, MA 01063 USA
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China
3.UMass Amherst, Program Organism & Evolutionary Biol, Amherst, MA 01003 USA
Recommended Citation
GB/T 7714
Huang, Jie,Katz, Laura A.. Nanochromosome Copy Number Does not Correlate with RNA Levels Though Patterns are Conserved between Strains of the Ciliate Morphospecies Chilodonella uncinata[J]. PROTIST,2014,165(4):445-451.
APA Huang, Jie,&Katz, Laura A..(2014).Nanochromosome Copy Number Does not Correlate with RNA Levels Though Patterns are Conserved between Strains of the Ciliate Morphospecies Chilodonella uncinata.PROTIST,165(4),445-451.
MLA Huang, Jie,et al."Nanochromosome Copy Number Does not Correlate with RNA Levels Though Patterns are Conserved between Strains of the Ciliate Morphospecies Chilodonella uncinata".PROTIST 165.4(2014):445-451.
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