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Molecular characterization of the cDNAs of two zona pellucida genes in the Chinese sturgeon, Acipenser sinensis Gray, 1835
Yue, H. -M.1,2; Cao, H.3; Chen, X. -H.1; Ye, H.1; Li, C. -J.1,2; Du, H.1,2
2014-12-01
Source PublicationJOURNAL OF APPLIED ICHTHYOLOGY
ISSN0175-8659
Volume30Issue:6Pages:1273-1281
AbstractThe Chinese sturgeon, Acipenser sinensis, is a large-sized panadromous cartilagous fish and requires more than ten years to reach sexual maturity. An understanding of the reproductive organs and fertilization process is a precondition for controlled reproduction of this endangered species. The developing oocyte is surrounded by an extracellular matrix composed of the zona pellucide (ZP) protein in several isoforms, all of which play important roles in fertilization and embryo protection. The present study reports the identification of two ZP genes, AsZPAX and AsZPB, in this fish. The full-length cDNAs of AsZPAX and AsZPB were 2950 base pairs (bp) and 1832bp, predicting proteins of 946 and 592 amino acid residues, respectively. Both proteins have a conserved ZP domain. In a gene tree, AsZPAX and AsZPB were respectively clustered with their counterparts in other vertebrates. Tissue distribution analysis demonstrated that the AsZPAX RNA was transcribed in both the ovary and the liver, while AsZPB was transcribed exclusively in the ovary. RNA in situ hybridization revealed apparent RNA expression of both AsZPAX and AsZPB in growing oocytes of the 4-year-old ovary.
SubtypeArticle
KeywordEgg Envelope Proteins Messenger Transport Organizer Seabream Sparus-aurata Vitelline Envelope Oryzias-latipes Mitochondrial Cloud Structural-analysis Xenopus Oocytes Expression Characterization Domain Proteins
DOI10.1111/jai.12586
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI ; ISTP
Language英语
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000345588500026
WOS KeywordEGG ENVELOPE PROTEINS ; MESSENGER TRANSPORT ORGANIZER ; SEABREAM SPARUS-AURATA ; VITELLINE ENVELOPE ; ORYZIAS-LATIPES ; MITOCHONDRIAL CLOUD ; STRUCTURAL-ANALYSIS ; XENOPUS OOCYTES ; EXPRESSION CHARACTERIZATION ; DOMAIN PROTEINS
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Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20290
Collection水生生物多样性与资源保护研究中心_期刊论文
Affiliation1.Chinese Acad Fisheries Sci, Yangtze River Fisheries Res Inst, Key Lab Freshwater Biodivers Conservat, Minist Agr China, Wuhan 430223, Peoples R China
2.Chinese Acad Fisheries Sci, Freshwater Fisheries Res Ctr, Wuxi, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
Recommended Citation
GB/T 7714
Yue, H. -M.,Cao, H.,Chen, X. -H.,et al. Molecular characterization of the cDNAs of two zona pellucida genes in the Chinese sturgeon, Acipenser sinensis Gray, 1835[J]. JOURNAL OF APPLIED ICHTHYOLOGY,2014,30(6):1273-1281.
APA Yue, H. -M.,Cao, H.,Chen, X. -H.,Ye, H.,Li, C. -J.,&Du, H..(2014).Molecular characterization of the cDNAs of two zona pellucida genes in the Chinese sturgeon, Acipenser sinensis Gray, 1835.JOURNAL OF APPLIED ICHTHYOLOGY,30(6),1273-1281.
MLA Yue, H. -M.,et al."Molecular characterization of the cDNAs of two zona pellucida genes in the Chinese sturgeon, Acipenser sinensis Gray, 1835".JOURNAL OF APPLIED ICHTHYOLOGY 30.6(2014):1273-1281.
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