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AtEXP2 Is Involved in Seed Germination and Abiotic Stress Response in Arabidopsis
Yan, An1; Wu, Minjie1; Yan, Limei1; Hu, Rui2; Ali, Imran1; Gan, Yinbo1; Gan, YB (reprint author), Zhejiang Univ, Zhejiang Key Lab Crop Germplasm, Dept Agron, Coll Agr & Biotechnol, Hangzhou 310003, Zhejiang, Peoples R China.
2014-01-03
Source PublicationPLOS ONE
ISSN1932-6203
Volume9Issue:1Pages:e85208
AbstractExpansins are cell wall proteins that promote cell wall loosening by inducing pH-dependent cell wall extension and stress relaxation. Expansins are required in a series of physiological developmental processes in higher plants such as seed germination. Here we identified an Arabidopsis expansin gene AtEXPA2 that is exclusively expressed in germinating seeds and the mutant shows delayed germination, suggesting that AtEXP2 is involved in controlling seed germination. Exogenous GA application increased the expression level of AtEXP2 during seed germination, while ABA application had no effect on AtEXP2 expression. Furthermore, the analysis of DELLA mutants show that RGL1, RGL2, RGA, GAI are all involved in repressing AtEXP2 expression, and RGL1 plays the most dominant role in controlling AtEXP2 expression. In stress response, exp2 mutant shows higher sensitivity than wild type in seed germination, while overexpression lines of AtEXP2 are less sensitive to salt stress and osmotic stress, exhibiting enhanced tolerance to stress treatment. Collectively, our results suggest that AtEXP2 is involved in the GA-mediated seed germination and confers salt stress and osmotic stress tolerance in Arabidopsis.; Expansins are cell wall proteins that promote cell wall loosening by inducing pH-dependent cell wall extension and stress relaxation. Expansins are required in a series of physiological developmental processes in higher plants such as seed germination. Here we identified an Arabidopsis expansin gene AtEXPA2 that is exclusively expressed in germinating seeds and the mutant shows delayed germination, suggesting that AtEXP2 is involved in controlling seed germination. Exogenous GA application increased the expression level of AtEXP2 during seed germination, while ABA application had no effect on AtEXP2 expression. Furthermore, the analysis of DELLA mutants show that RGL1, RGL2, RGA, GAI are all involved in repressing AtEXP2 expression, and RGL1 plays the most dominant role in controlling AtEXP2 expression. In stress response, exp2 mutant shows higher sensitivity than wild type in seed germination, while overexpression lines of AtEXP2 are less sensitive to salt stress and osmotic stress, exhibiting enhanced tolerance to stress treatment. Collectively, our results suggest that AtEXP2 is involved in the GA-mediated seed germination and confers salt stress and osmotic stress tolerance in Arabidopsis.
SubtypeArticle
KeywordCell-wall Extensibility Expansin Gene Gibberellin Biosynthesis Molecular-cloning Multigene Family Leaf Development Expression Thaliana Proteins Growth
Department[Yan, An; Wu, Minjie; Yan, Limei; Ali, Imran; Gan, Yinbo] Zhejiang Univ, Zhejiang Key Lab Crop Germplasm, Dept Agron, Coll Agr & Biotechnol, Hangzhou 310003, Zhejiang, Peoples R China; [Hu, Rui] Chinese Acad Sci, Inst Hydrobiol, Wuhan, Hubei, Peoples R China
DOI10.1371/journal.pone.0085208
WOS HeadingsScience & Technology
Funding OrganizationInternational Scientific and Technological Cooperation Project of Science and Technology Department of Zhejiang Province [2013C34G2010017]; International Scientific and Technological Cooperation Project of the Ministry of Science and Technology of China [2010DFA34430]; National Natural Science Foundation of China [31370215, 31228002]; Zhejiang Provincial Natural Science Foundation of China [Z31100041] ; International Scientific and Technological Cooperation Project of Science and Technology Department of Zhejiang Province [2013C34G2010017]; International Scientific and Technological Cooperation Project of the Ministry of Science and Technology of China [2010DFA34430]; National Natural Science Foundation of China [31370215, 31228002]; Zhejiang Provincial Natural Science Foundation of China [Z31100041] ; International Scientific and Technological Cooperation Project of Science and Technology Department of Zhejiang Province [2013C34G2010017]; International Scientific and Technological Cooperation Project of the Ministry of Science and Technology of China [2010DFA34430]; National Natural Science Foundation of China [31370215, 31228002]; Zhejiang Provincial Natural Science Foundation of China [Z31100041] ; International Scientific and Technological Cooperation Project of Science and Technology Department of Zhejiang Province [2013C34G2010017]; International Scientific and Technological Cooperation Project of the Ministry of Science and Technology of China [2010DFA34430]; National Natural Science Foundation of China [31370215, 31228002]; Zhejiang Provincial Natural Science Foundation of China [Z31100041]
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000329460800082
WOS KeywordCELL-WALL EXTENSIBILITY ; EXPANSIN GENE ; GIBBERELLIN BIOSYNTHESIS ; MOLECULAR-CLONING ; MULTIGENE FAMILY ; LEAF DEVELOPMENT ; EXPRESSION ; THALIANA ; PROTEINS ; GROWTH
Funding OrganizationInternational Scientific and Technological Cooperation Project of Science and Technology Department of Zhejiang Province [2013C34G2010017]; International Scientific and Technological Cooperation Project of the Ministry of Science and Technology of China [2010DFA34430]; National Natural Science Foundation of China [31370215, 31228002]; Zhejiang Provincial Natural Science Foundation of China [Z31100041] ; International Scientific and Technological Cooperation Project of Science and Technology Department of Zhejiang Province [2013C34G2010017]; International Scientific and Technological Cooperation Project of the Ministry of Science and Technology of China [2010DFA34430]; National Natural Science Foundation of China [31370215, 31228002]; Zhejiang Provincial Natural Science Foundation of China [Z31100041] ; International Scientific and Technological Cooperation Project of Science and Technology Department of Zhejiang Province [2013C34G2010017]; International Scientific and Technological Cooperation Project of the Ministry of Science and Technology of China [2010DFA34430]; National Natural Science Foundation of China [31370215, 31228002]; Zhejiang Provincial Natural Science Foundation of China [Z31100041] ; International Scientific and Technological Cooperation Project of Science and Technology Department of Zhejiang Province [2013C34G2010017]; International Scientific and Technological Cooperation Project of the Ministry of Science and Technology of China [2010DFA34430]; National Natural Science Foundation of China [31370215, 31228002]; Zhejiang Provincial Natural Science Foundation of China [Z31100041]
Citation statistics
Cited Times:37[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20125
Collection分析测试中心_期刊论文
Corresponding AuthorGan, YB (reprint author), Zhejiang Univ, Zhejiang Key Lab Crop Germplasm, Dept Agron, Coll Agr & Biotechnol, Hangzhou 310003, Zhejiang, Peoples R China.
Affiliation1.Zhejiang Univ, Zhejiang Key Lab Crop Germplasm, Dept Agron, Coll Agr & Biotechnol, Hangzhou 310003, Zhejiang, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Yan, An,Wu, Minjie,Yan, Limei,et al. AtEXP2 Is Involved in Seed Germination and Abiotic Stress Response in Arabidopsis[J]. PLOS ONE,2014,9(1):e85208.
APA Yan, An.,Wu, Minjie.,Yan, Limei.,Hu, Rui.,Ali, Imran.,...&Gan, YB .(2014).AtEXP2 Is Involved in Seed Germination and Abiotic Stress Response in Arabidopsis.PLOS ONE,9(1),e85208.
MLA Yan, An,et al."AtEXP2 Is Involved in Seed Germination and Abiotic Stress Response in Arabidopsis".PLOS ONE 9.1(2014):e85208.
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