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题名: AtEXP2 Is Involved in Seed Germination and Abiotic Stress Response in Arabidopsis
作者: Yan, An1; Wu, Minjie1; Yan, Limei1; Hu, Rui2; Ali, Imran1; Gan, Yinbo1
通讯作者: Gan, YB (reprint author), Zhejiang Univ, Zhejiang Key Lab Crop Germplasm, Dept Agron, Coll Agr & Biotechnol, Hangzhou 310003, Zhejiang, Peoples R China.
关键词: CELL-WALL EXTENSIBILITY ; EXPANSIN GENE ; GIBBERELLIN BIOSYNTHESIS ; MOLECULAR-CLONING ; MULTIGENE FAMILY ; LEAF DEVELOPMENT ; EXPRESSION ; THALIANA ; PROTEINS ; GROWTH
刊名: PLOS ONE
发表日期: 2014-01-03
DOI: 10.1371/journal.pone.0085208
卷: 9, 期:1, 页:e85208
收录类别: SCI
文章类型: Article
部门归属: [Yan, An; Wu, Minjie; Yan, Limei; Ali, Imran; Gan, Yinbo] Zhejiang Univ, Zhejiang Key Lab Crop Germplasm, Dept Agron, Coll Agr & Biotechnol, Hangzhou 310003, Zhejiang, Peoples R China; [Hu, Rui] Chinese Acad Sci, Inst Hydrobiol, Wuhan, Hubei, Peoples R China
WOS标题词: Science & Technology
资助者: International Scientific and Technological Cooperation Project of Science and Technology Department of Zhejiang Province [2013C34G2010017]; International Scientific and Technological Cooperation Project of the Ministry of Science and Technology of China [2010DFA34430]; National Natural Science Foundation of China [31370215, 31228002]; Zhejiang Provincial Natural Science Foundation of China [Z31100041]
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
摘要: Expansins are cell wall proteins that promote cell wall loosening by inducing pH-dependent cell wall extension and stress relaxation. Expansins are required in a series of physiological developmental processes in higher plants such as seed germination. Here we identified an Arabidopsis expansin gene AtEXPA2 that is exclusively expressed in germinating seeds and the mutant shows delayed germination, suggesting that AtEXP2 is involved in controlling seed germination. Exogenous GA application increased the expression level of AtEXP2 during seed germination, while ABA application had no effect on AtEXP2 expression. Furthermore, the analysis of DELLA mutants show that RGL1, RGL2, RGA, GAI are all involved in repressing AtEXP2 expression, and RGL1 plays the most dominant role in controlling AtEXP2 expression. In stress response, exp2 mutant shows higher sensitivity than wild type in seed germination, while overexpression lines of AtEXP2 are less sensitive to salt stress and osmotic stress, exhibiting enhanced tolerance to stress treatment. Collectively, our results suggest that AtEXP2 is involved in the GA-mediated seed germination and confers salt stress and osmotic stress tolerance in Arabidopsis.
英文摘要: Expansins are cell wall proteins that promote cell wall loosening by inducing pH-dependent cell wall extension and stress relaxation. Expansins are required in a series of physiological developmental processes in higher plants such as seed germination. Here we identified an Arabidopsis expansin gene AtEXPA2 that is exclusively expressed in germinating seeds and the mutant shows delayed germination, suggesting that AtEXP2 is involved in controlling seed germination. Exogenous GA application increased the expression level of AtEXP2 during seed germination, while ABA application had no effect on AtEXP2 expression. Furthermore, the analysis of DELLA mutants show that RGL1, RGL2, RGA, GAI are all involved in repressing AtEXP2 expression, and RGL1 plays the most dominant role in controlling AtEXP2 expression. In stress response, exp2 mutant shows higher sensitivity than wild type in seed germination, while overexpression lines of AtEXP2 are less sensitive to salt stress and osmotic stress, exhibiting enhanced tolerance to stress treatment. Collectively, our results suggest that AtEXP2 is involved in the GA-mediated seed germination and confers salt stress and osmotic stress tolerance in Arabidopsis.
关键词[WOS]: CELL-WALL EXTENSIBILITY ; EXPANSIN GENE ; GIBBERELLIN BIOSYNTHESIS ; MOLECULAR-CLONING ; MULTIGENE FAMILY ; LEAF DEVELOPMENT ; EXPRESSION ; THALIANA ; PROTEINS ; GROWTH
语种: 英语
WOS记录号: WOS:000329460800082
ISSN号: 1932-6203
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/20125
Appears in Collections:分析测试中心_期刊论文

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作者单位: 1.Zhejiang Univ, Zhejiang Key Lab Crop Germplasm, Dept Agron, Coll Agr & Biotechnol, Hangzhou 310003, Zhejiang, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Hubei, Peoples R China

Recommended Citation:
Yan, An; Wu, Minjie; Yan, Limei; Hu, Rui; Ali, Imran; Gan, Yinbo.AtEXP2 Is Involved in Seed Germination and Abiotic Stress Response in Arabidopsis,PLOS ONE,2014,9(1):e85208
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