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Constructing a new integrated genetic linkage map and mapping quantitative trait loci for vegetative mycelium growth rate in Lentinula edodes
Gong, Wen-Bing1,2; Liu, Wei2,3; Lu, Ying-Ying2,4; Bian, Yin-Bing1,2; Zhou, Yan1,2; Kwan, Hoi Shan5; Cheung, Man Kit5; Xiao, Yang1,2; Xiao, Y (reprint author), Huazhong Agr Univ, Key Lab Agromicrobial Resource & Dev, Minist Agr, Wuhan 430070, Hubei Province, Peoples R China.
2014-03-01
Source PublicationFUNGAL BIOLOGY
ISSN1878-6146
Volume118Issue:3Pages:295-308
AbstractThe most saturated linkage map for Lentinula edodes to date was constructed based on a mono-. karyotic population of 146 single spore isolates (SSIs) using sequence-related amplified polymorphism (SRAP), target region amplification polymorphism (TRAP), insertion deletion (InDel) markers, and the mating-type loci. Five hundred and twenty-four markers were located on 13 linkage groups (LGs). The map spanned a total length of 1006.1 cM, with an average marker spacing of 2.0 cM. Quantitative trait loci (QTLs) mapping was utilized to uncover the loci regulating and controlling the vegetative mycelium growth rate on various synthetic media, and complex medium for commercial cultivation of L. edodes. Two and 13 putative QTLs, identified respectively in the monokaryotic population and two testcross dikaryotic populations, were mapped on seven different LGs. Several vegetative mycelium growth rate-related QTLs uncovered here were clustered on LG4 (Qmgr1, Qdgr1, Qdgr2 and Qdgr9) and LG6 (Qdgr3, Qdgr4 and Qdgr5), implying the presence of main genomic areas responsible for growth rate regulation and control. The QTL hotspot region on LG4 was found to be in close proximity to:the region containing the mating-type A (MAT-A) locus. Moreover, Qdgr2 on LG4 was detected on different media, contributing 8.07%-23.71% of the phenotypic variation. The present study provides essential information for QTL mapping and marker-assisted selection (MAS) in L. edodes. (C) 2014 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.; The most saturated linkage map for Lentinula edodes to date was constructed based on a mono-. karyotic population of 146 single spore isolates (SSIs) using sequence-related amplified polymorphism (SRAP), target region amplification polymorphism (TRAP), insertion deletion (InDel) markers, and the mating-type loci. Five hundred and twenty-four markers were located on 13 linkage groups (LGs). The map spanned a total length of 1006.1 cM, with an average marker spacing of 2.0 cM. Quantitative trait loci (QTLs) mapping was utilized to uncover the loci regulating and controlling the vegetative mycelium growth rate on various synthetic media, and complex medium for commercial cultivation of L. edodes. Two and 13 putative QTLs, identified respectively in the monokaryotic population and two testcross dikaryotic populations, were mapped on seven different LGs. Several vegetative mycelium growth rate-related QTLs uncovered here were clustered on LG4 (Qmgr1, Qdgr1, Qdgr2 and Qdgr9) and LG6 (Qdgr3, Qdgr4 and Qdgr5), implying the presence of main genomic areas responsible for growth rate regulation and control. The QTL hotspot region on LG4 was found to be in close proximity to:the region containing the mating-type A (MAT-A) locus. Moreover, Qdgr2 on LG4 was detected on different media, contributing 8.07%-23.71% of the phenotypic variation. The present study provides essential information for QTL mapping and marker-assisted selection (MAS) in L. edodes. (C) 2014 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordGenetic Linkage Map Quantitative Trait Loci Shiitake Mushroom Vegetative Mycelium Growth Rate
Department[Gong, Wen-Bing; Bian, Yin-Bing; Zhou, Yan; Xiao, Yang] Huazhong Agr Univ, Key Lab Agromicrobial Resource & Dev, Minist Agr, Wuhan 430070, Hubei Province, Peoples R China; [Gong, Wen-Bing; Liu, Wei; Lu, Ying-Ying; Bian, Yin-Bing; Zhou, Yan; Xiao, Yang] Huazhong Agr Univ, Inst Appl Mycol, Wuhan 430070, Hubei Province, Peoples R China; [Liu, Wei] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China; [Lu, Ying-Ying] Guizhou Acad Agr Sci, Inst Crop Genet Resource, Guiyang 550006, Guizhou Provinc, Peoples R China; [Kwan, Hoi Shan; Cheung, Man Kit] Chinese Univ Hong Kong, Sch Life Sci, Hong Kong, Hong Kong, Peoples R China
DOI10.1016/j.funbio.2014.01.001
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [31000929] ; National Natural Science Foundation of China [31000929] ; National Natural Science Foundation of China [31000929] ; National Natural Science Foundation of China [31000929]
Indexed BySCI
Language英语
WOS Research AreaMycology
WOS SubjectMycology
WOS IDWOS:000333785900003
WOS KeywordBASIDIOMYCETE PLEUROTUS-OSTREATUS ; AGARICUS-BISPORUS ; MATING-TYPE ; AFLP MARKERS ; POLYMORPHISM MARKERS ; SHIITAKE MUSHROOM ; CAP COLOR ; IDENTIFICATION ; FUNGUS ; REGION
Funding OrganizationNational Natural Science Foundation of China [31000929] ; National Natural Science Foundation of China [31000929] ; National Natural Science Foundation of China [31000929] ; National Natural Science Foundation of China [31000929]
Citation statistics
Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/20123
Collection分析测试中心_期刊论文
Corresponding AuthorXiao, Y (reprint author), Huazhong Agr Univ, Key Lab Agromicrobial Resource & Dev, Minist Agr, Wuhan 430070, Hubei Province, Peoples R China.
Affiliation1.Huazhong Agr Univ, Key Lab Agromicrobial Resource & Dev, Minist Agr, Wuhan 430070, Hubei Province, Peoples R China
2.Huazhong Agr Univ, Inst Appl Mycol, Wuhan 430070, Hubei Province, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China
4.Guizhou Acad Agr Sci, Inst Crop Genet Resource, Guiyang 550006, Guizhou Provinc, Peoples R China
5.Chinese Univ Hong Kong, Sch Life Sci, Hong Kong, Hong Kong, Peoples R China
Recommended Citation
GB/T 7714
Gong, Wen-Bing,Liu, Wei,Lu, Ying-Ying,et al. Constructing a new integrated genetic linkage map and mapping quantitative trait loci for vegetative mycelium growth rate in Lentinula edodes[J]. FUNGAL BIOLOGY,2014,118(3):295-308.
APA Gong, Wen-Bing.,Liu, Wei.,Lu, Ying-Ying.,Bian, Yin-Bing.,Zhou, Yan.,...&Xiao, Y .(2014).Constructing a new integrated genetic linkage map and mapping quantitative trait loci for vegetative mycelium growth rate in Lentinula edodes.FUNGAL BIOLOGY,118(3),295-308.
MLA Gong, Wen-Bing,et al."Constructing a new integrated genetic linkage map and mapping quantitative trait loci for vegetative mycelium growth rate in Lentinula edodes".FUNGAL BIOLOGY 118.3(2014):295-308.
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