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题名: Constructing a new integrated genetic linkage map and mapping quantitative trait loci for vegetative mycelium growth rate in Lentinula edodes
作者: Gong, Wen-Bing1, 2; Liu, Wei2, 3; Lu, Ying-Ying2, 4; Bian, Yin-Bing1, 2; Zhou, Yan1, 2; Kwan, Hoi Shan5; Cheung, Man Kit5; Xiao, Yang1, 2
通讯作者: Xiao, Y (reprint author), Huazhong Agr Univ, Key Lab Agromicrobial Resource & Dev, Minist Agr, Wuhan 430070, Hubei Province, Peoples R China.
关键词: Genetic linkage map ; Quantitative trait loci ; Shiitake mushroom ; Vegetative mycelium growth rate
刊名: FUNGAL BIOLOGY
发表日期: 2014-03-01
DOI: 10.1016/j.funbio.2014.01.001
卷: 118, 期:3, 页:295-308
收录类别: SCI
文章类型: Article
部门归属: [Gong, Wen-Bing; Bian, Yin-Bing; Zhou, Yan; Xiao, Yang] Huazhong Agr Univ, Key Lab Agromicrobial Resource & Dev, Minist Agr, Wuhan 430070, Hubei Province, Peoples R China; [Gong, Wen-Bing; Liu, Wei; Lu, Ying-Ying; Bian, Yin-Bing; Zhou, Yan; Xiao, Yang] Huazhong Agr Univ, Inst Appl Mycol, Wuhan 430070, Hubei Province, Peoples R China; [Liu, Wei] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China; [Lu, Ying-Ying] Guizhou Acad Agr Sci, Inst Crop Genet Resource, Guiyang 550006, Guizhou Provinc, Peoples R China; [Kwan, Hoi Shan; Cheung, Man Kit] Chinese Univ Hong Kong, Sch Life Sci, Hong Kong, Hong Kong, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China [31000929]
类目[WOS]: Mycology
研究领域[WOS]: Mycology
摘要: The most saturated linkage map for Lentinula edodes to date was constructed based on a mono-. karyotic population of 146 single spore isolates (SSIs) using sequence-related amplified polymorphism (SRAP), target region amplification polymorphism (TRAP), insertion deletion (InDel) markers, and the mating-type loci. Five hundred and twenty-four markers were located on 13 linkage groups (LGs). The map spanned a total length of 1006.1 cM, with an average marker spacing of 2.0 cM. Quantitative trait loci (QTLs) mapping was utilized to uncover the loci regulating and controlling the vegetative mycelium growth rate on various synthetic media, and complex medium for commercial cultivation of L. edodes. Two and 13 putative QTLs, identified respectively in the monokaryotic population and two testcross dikaryotic populations, were mapped on seven different LGs. Several vegetative mycelium growth rate-related QTLs uncovered here were clustered on LG4 (Qmgr1, Qdgr1, Qdgr2 and Qdgr9) and LG6 (Qdgr3, Qdgr4 and Qdgr5), implying the presence of main genomic areas responsible for growth rate regulation and control. The QTL hotspot region on LG4 was found to be in close proximity to:the region containing the mating-type A (MAT-A) locus. Moreover, Qdgr2 on LG4 was detected on different media, contributing 8.07%-23.71% of the phenotypic variation. The present study provides essential information for QTL mapping and marker-assisted selection (MAS) in L. edodes. (C) 2014 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
英文摘要: The most saturated linkage map for Lentinula edodes to date was constructed based on a mono-. karyotic population of 146 single spore isolates (SSIs) using sequence-related amplified polymorphism (SRAP), target region amplification polymorphism (TRAP), insertion deletion (InDel) markers, and the mating-type loci. Five hundred and twenty-four markers were located on 13 linkage groups (LGs). The map spanned a total length of 1006.1 cM, with an average marker spacing of 2.0 cM. Quantitative trait loci (QTLs) mapping was utilized to uncover the loci regulating and controlling the vegetative mycelium growth rate on various synthetic media, and complex medium for commercial cultivation of L. edodes. Two and 13 putative QTLs, identified respectively in the monokaryotic population and two testcross dikaryotic populations, were mapped on seven different LGs. Several vegetative mycelium growth rate-related QTLs uncovered here were clustered on LG4 (Qmgr1, Qdgr1, Qdgr2 and Qdgr9) and LG6 (Qdgr3, Qdgr4 and Qdgr5), implying the presence of main genomic areas responsible for growth rate regulation and control. The QTL hotspot region on LG4 was found to be in close proximity to:the region containing the mating-type A (MAT-A) locus. Moreover, Qdgr2 on LG4 was detected on different media, contributing 8.07%-23.71% of the phenotypic variation. The present study provides essential information for QTL mapping and marker-assisted selection (MAS) in L. edodes. (C) 2014 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
关键词[WOS]: BASIDIOMYCETE PLEUROTUS-OSTREATUS ; AGARICUS-BISPORUS ; MATING-TYPE ; AFLP MARKERS ; POLYMORPHISM MARKERS ; SHIITAKE MUSHROOM ; CAP COLOR ; IDENTIFICATION ; FUNGUS ; REGION
语种: 英语
WOS记录号: WOS:000333785900003
ISSN号: 1878-6146
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/20123
Appears in Collections:分析测试中心_期刊论文

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作者单位: 1.Huazhong Agr Univ, Key Lab Agromicrobial Resource & Dev, Minist Agr, Wuhan 430070, Hubei Province, Peoples R China
2.Huazhong Agr Univ, Inst Appl Mycol, Wuhan 430070, Hubei Province, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China
4.Guizhou Acad Agr Sci, Inst Crop Genet Resource, Guiyang 550006, Guizhou Provinc, Peoples R China
5.Chinese Univ Hong Kong, Sch Life Sci, Hong Kong, Hong Kong, Peoples R China

Recommended Citation:
Gong, Wen-Bing; Liu, Wei; Lu, Ying-Ying; Bian, Yin-Bing; Zhou, Yan; Kwan, Hoi Shan; Cheung, Man Kit; Xiao, Yang.Constructing a new integrated genetic linkage map and mapping quantitative trait loci for vegetative mycelium growth rate in Lentinula edodes,FUNGAL BIOLOGY,2014,118(3):295-308
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