| Nannochloropsis Genomes Reveal Evolution of Microalgal Oleaginous Traits | |
| Wang, Dongmei1,2; Ning, Kang1,2; Li, Jing1,2; Hu, Jianqiang1,2; Han, Danxiang3; Wang, Hui4; Zeng, Xiaowei1,2; Jing, Xiaoyan1,2; Zhou, Qian1,2; Su, Xiaoquan1,2; Chang, Xingzhi1,2; Wang, Anhui1,2; Wang, Wei1,2; Jia, Jing1,2; Wei, Li1,2; Xin, Yi1,2; Qiao, Yinghe1,2; Huang, Ranran1,2; Chen, Jie1,2; Han, Bo1,2; Yoon, Kangsup5; Hill, Russell T.4; Zohar, Yonathan6,7; Chen, Feng4; Hu, Qiang5; Xu, Jian1,2; Wang, DM (reprint author), Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, CAS Key Lab Biofuels, Shandong Key Lab Energy Genet, Qingdao, Shandong, Peoples R China. | |
| 2014 | |
| Source Publication | PLOS GENETICS
 |
| ISSN | 1553-7390 |
| Volume | 10Issue:1Pages:e1004094 |
| Abstract | Oleaginous microalgae are promising feedstock for biofuels, yet the genetic diversity, origin and evolution of oleaginous traits remain largely unknown. Here we present a detailed phylogenomic analysis of five oleaginous Nannochloropsis species (a total of six strains) and one time-series transcriptome dataset for triacylglycerol (TAG) synthesis on one representative strain. Despite small genome sizes, high coding potential and relative paucity of mobile elements, the genomes feature small cores of ca. 2,700 protein-coding genes and a large pan-genome of >38,000 genes. The six genomes share key oleaginous traits, such as the enrichment of selected lipid biosynthesis genes and certain glycoside hydrolase genes that potentially shift carbon flux from chrysolaminaran to TAG synthesis. The eleven type II diacylglycerol acyltransferase genes (DGAT-2) in every strain, each expressed during TAG synthesis, likely originated from three ancient genomes, including the secondary endosymbiosis host and the engulfed green and red algae. Horizontal gene transfers were inferred in most lipid synthesis nodes with expanded gene doses and many glycoside hydrolase genes. Thus multiple genome pooling and horizontal genetic exchange, together with selective inheritance of lipid synthesis genes and species-specific gene loss, have led to the enormous genetic apparatus for oleaginousness and the wide genomic divergence among present-day Nannochloropsis. These findings have important implications in the screening and genetic engineering of microalgae for biofuels.; Oleaginous microalgae are promising feedstock for biofuels, yet the genetic diversity, origin and evolution of oleaginous traits remain largely unknown. Here we present a detailed phylogenomic analysis of five oleaginous Nannochloropsis species (a total of six strains) and one time-series transcriptome dataset for triacylglycerol (TAG) synthesis on one representative strain. Despite small genome sizes, high coding potential and relative paucity of mobile elements, the genomes feature small cores of ca. 2,700 protein-coding genes and a large pan-genome of >38,000 genes. The six genomes share key oleaginous traits, such as the enrichment of selected lipid biosynthesis genes and certain glycoside hydrolase genes that potentially shift carbon flux from chrysolaminaran to TAG synthesis. The eleven type II diacylglycerol acyltransferase genes (DGAT-2) in every strain, each expressed during TAG synthesis, likely originated from three ancient genomes, including the secondary endosymbiosis host and the engulfed green and red algae. Horizontal gene transfers were inferred in most lipid synthesis nodes with expanded gene doses and many glycoside hydrolase genes. Thus multiple genome pooling and horizontal genetic exchange, together with selective inheritance of lipid synthesis genes and species-specific gene loss, have led to the enormous genetic apparatus for oleaginousness and the wide genomic divergence among present-day Nannochloropsis. These findings have important implications in the screening and genetic engineering of microalgae for biofuels. |
| Subtype | Article |
| Keyword | Gene Amplification Bacteria Sequence Protein Adaptation Expression Eukaryote Biofuels Yeast Rates |
| Department | [Wang, Dongmei; Ning, Kang; Li, Jing; Hu, Jianqiang; Zeng, Xiaowei; Jing, Xiaoyan; Zhou, Qian; Su, Xiaoquan; Chang, Xingzhi; Wang, Anhui; Wang, Wei; Jia, Jing; Wei, Li; Xin, Yi; Qiao, Yinghe; Huang, Ranran; Chen, Jie; Han, Bo; Xu, Jian] Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, CAS Key Lab Biofuels, Shandong Key Lab Energy Genet, Qingdao, Shandong, Peoples R China; [Wang, Dongmei; Ning, Kang; Li, Jing; Hu, Jianqiang; Zeng, Xiaowei; Jing, Xiaoyan; Zhou, Qian; Su, Xiaoquan; Chang, Xingzhi; Wang, Anhui; Wang, Wei; Jia, Jing; Wei, Li; Xin, Yi; Qiao, Yinghe; Huang, Ranran; Chen, Jie; Han, Bo; Xu, Jian] Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, BioEnergy Genome Ctr, Qingdao, Shandong, Peoples R China; [Han, Danxiang] Arizona State Univ, Coll Technol & Innovat, Lab Algae Res & Biotechnol, Mesa, AZ USA; [Wang, Hui; Hill, Russell T.; Chen, Feng] Univ Maryland, Ctr Environm Sci, Inst Marine & Environm Technol, Baltimore, MD 21201 USA; [Yoon, Kangsup; Hu, Qiang] Chinese Acad Sci, Inst Hydrobiol, Ctr Microalgal Biotechnol & Biofuels, Wuhan, Hubei, Peoples R China; [Zohar, Yonathan] Univ Maryland Baltimore Cty, Inst Marine Environm Technol, Baltimore, MD 21228 USA; [Zohar, Yonathan] Univ Maryland Baltimore Cty, Dept Marine Biotechnol, Baltimore, MD 21228 USA |
| DOI | 10.1371/journal.pgen.1004094 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | Ministry of Science and Technology of China [2012CB721101, 2012AA02A707, 2011CB200902]; National Natural Science Foundation of China [31010103907, 91231205, 61103167]; Chinese Academy of Sciences ; Ministry of Science and Technology of China [2012CB721101, 2012AA02A707, 2011CB200902]; National Natural Science Foundation of China [31010103907, 91231205, 61103167]; Chinese Academy of Sciences ; Ministry of Science and Technology of China [2012CB721101, 2012AA02A707, 2011CB200902]; National Natural Science Foundation of China [31010103907, 91231205, 61103167]; Chinese Academy of Sciences ; Ministry of Science and Technology of China [2012CB721101, 2012AA02A707, 2011CB200902]; National Natural Science Foundation of China [31010103907, 91231205, 61103167]; Chinese Academy of Sciences |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Genetics & Heredity |
| WOS Subject | Genetics & Heredity |
| WOS ID | WOS:000336525000053 |
| WOS Keyword | GENE AMPLIFICATION ; BACTERIA ; SEQUENCE ; PROTEIN ; ADAPTATION ; EXPRESSION ; EUKARYOTE ; BIOFUELS ; YEAST ; RATES |
| Funding Organization | Ministry of Science and Technology of China [2012CB721101, 2012AA02A707, 2011CB200902]; National Natural Science Foundation of China [31010103907, 91231205, 61103167]; Chinese Academy of Sciences ; Ministry of Science and Technology of China [2012CB721101, 2012AA02A707, 2011CB200902]; National Natural Science Foundation of China [31010103907, 91231205, 61103167]; Chinese Academy of Sciences ; Ministry of Science and Technology of China [2012CB721101, 2012AA02A707, 2011CB200902]; National Natural Science Foundation of China [31010103907, 91231205, 61103167]; Chinese Academy of Sciences ; Ministry of Science and Technology of China [2012CB721101, 2012AA02A707, 2011CB200902]; National Natural Science Foundation of China [31010103907, 91231205, 61103167]; Chinese Academy of Sciences |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/20122 |
| Collection | 藻类生物技术和生物能源研发中心_期刊论文 |
| Corresponding Author | Wang, DM (reprint author), Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, CAS Key Lab Biofuels, Shandong Key Lab Energy Genet, Qingdao, Shandong, Peoples R China. |
| Affiliation | 1.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, CAS Key Lab Biofuels, Shandong Key Lab Energy Genet, Qingdao, Shandong, Peoples R China 2.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, BioEnergy Genome Ctr, Qingdao, Shandong, Peoples R China 3.Arizona State Univ, Coll Technol & Innovat, Lab Algae Res & Biotechnol, Mesa, AZ USA 4.Univ Maryland, Ctr Environm Sci, Inst Marine & Environm Technol, Baltimore, MD 21201 USA 5.Chinese Acad Sci, Inst Hydrobiol, Ctr Microalgal Biotechnol & Biofuels, Wuhan, Hubei, Peoples R China 6.Univ Maryland Baltimore Cty, Inst Marine Environm Technol, Baltimore, MD 21228 USA 7.Univ Maryland Baltimore Cty, Dept Marine Biotechnol, Baltimore, MD 21228 USA |
| Recommended Citation GB/T 7714 | Wang, Dongmei,Ning, Kang,Li, Jing,et al. Nannochloropsis Genomes Reveal Evolution of Microalgal Oleaginous Traits[J]. PLOS GENETICS,2014,10(1):e1004094. |
| APA | Wang, Dongmei.,Ning, Kang.,Li, Jing.,Hu, Jianqiang.,Han, Danxiang.,...&Wang, DM .(2014).Nannochloropsis Genomes Reveal Evolution of Microalgal Oleaginous Traits.PLOS GENETICS,10(1),e1004094. |
| MLA | Wang, Dongmei,et al."Nannochloropsis Genomes Reveal Evolution of Microalgal Oleaginous Traits".PLOS GENETICS 10.1(2014):e1004094. |
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